细胞衰老中染色质分布的不平衡指定了副斑动力学

IF 10.1 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genome Biology Pub Date : 2025-09-02 DOI:10.1186/s13059-025-03757-6

Joonwoo Lee, Jinmi Choi, Jeongeun Park, Seonduk Lee, Eun-Jung Cho, Youngdae Gwon

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引用次数: 0

摘要

细胞衰老伴随着广泛的基因组重组，如衰老相关的异染色质病灶和染色质间室的扩大，最终影响基因表达。在这里，我们证明了衰老细胞中染色质结构的变化驱动了副斑的相位行为和运动的显著改变，副斑是一种染色质间室凝聚物。我们观察到含有NONO和NEAT1_2的副斑的数量、大小和延伸率都增加了，这是由这些成分的增加所驱动的，这与纵向生长的胶束化模型相一致，而不是凝聚聚结。旁斑运动增强与细胞衰老中发现的hp1 α介导的异染色质凝聚和染色质间扩张有关。

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Imbalanced chromatin distribution in cellular senescence specifies paraspeckle dynamics

Cellular senescence is accompanied by extensive genomic reorganization, such as senescence-associated heterochromatin foci and expanded interchromatin compartments, to ultimately affect gene expression. Here, we demonstrate that chromatin structural changes in senescent cells drive significant alterations in the phase behavior and motility of paraspeckles, a type of interchromatin compartment condensate. We observe increased numbers, size, and elongation of paraspeckles harboring NONO and NEAT1_2, driven by elevated levels of those components, consistent with the micellization model of longitudinal growth rather than condensate coalescence. Enhanced paraspeckle motility is associated with HP1α-mediated heterochromatin condensation and interchromatin expansion found in cellular senescence.

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来源期刊

Genome Biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

21.00

自引率

3.30%

发文量

241

审稿时长

2 months

期刊介绍： Genome Biology stands as a premier platform for exceptional research across all domains of biology and biomedicine, explored through a genomic and post-genomic lens. With an impressive impact factor of 12.3 (2022),* the journal secures its position as the 3rd-ranked research journal in the Genetics and Heredity category and the 2nd-ranked research journal in the Biotechnology and Applied Microbiology category by Thomson Reuters. Notably, Genome Biology holds the distinction of being the highest-ranked open-access journal in this category. Our dedicated team of highly trained in-house Editors collaborates closely with our esteemed Editorial Board of international experts, ensuring the journal remains on the forefront of scientific advances and community standards. Regular engagement with researchers at conferences and institute visits underscores our commitment to staying abreast of the latest developments in the field.