6种黄酮类化合物酚羟基质子转移与抗氧化活性的相关性研究

IF 2.5 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochimica et biophysica acta. Biomembranes Pub Date : 2025-08-19 DOI:10.1016/j.bbamem.2025.184444

Shuangmei Gong , Changying Yang , Min Li , Xiaobo Li

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引用次数: 0

摘要

本研究研究了6种黄酮类化合物在不同环境下的光物理性质，揭示了它们的pKa变化（例如，槲皮素在水中：7.364比在囊泡中：9.329），并证明了囊泡/脂质体系统通过减缓质子转移和氧化的保护作用。金属离子络合优先发生在特定的羟基酮位点（槲皮素/山奈酚的3- oh -4-酮，芹菜素/黄芩素的5- oh -4-酮），而酚羟基排列和双键等结构特征显著影响这些相互作用。重要的是，这项工作确定了与脂质体相比，囊泡系统提供了更好的保护，防止质子转移和氧化。ANS荧光猝灭研究进一步表征了它们的分子行为，为黄酮类化合物的稳定机制和药理活性基础提供了重要的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Study on the correlation between proton transfer of phenolic hydroxyl groups of 6 flavonoid compounds and their antioxidant activities

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Study on the correlation between proton transfer of phenolic hydroxyl groups of 6 flavonoid compounds and their antioxidant activities

This study investigated six flavonoids' photophysical properties in various environments, revealing their pKa variations (e.g., quercetin: 7.364 in water vs 9.329 in vesicles) and demonstrating vesicle/liposome systems' protective effects by slowing proton transfer and oxidation. Metal ion complexation occurred preferentially at specific hydroxyl-keto sites (3-OH-4-keto for quercetin/kaempferol, 5-OH-4-keto for apigenin/baicalein), while structural features like phenolic hydroxyl arrangement and double bonds significantly influenced these interactions. Importantly, the work established that vesicular systems provide superior protection against proton transfer and oxidation compared to liposomes.ANS fluorescence quenching studies further characterized their molecular behaviors, providing key insights into flavonoid stabilization mechanisms and pharmacological activity foundations.

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来源期刊

Biochimica et biophysica acta. Biomembranes 生物-生化与分子生物学

CiteScore

8.20

自引率

5.90%

发文量

175

审稿时长

2.3 months

期刊介绍： BBA Biomembranes has its main focus on membrane structure, function and biomolecular organization, membrane proteins, receptors, channels and anchors, fluidity and composition, model membranes and liposomes, membrane surface studies and ligand interactions, transport studies, and membrane dynamics.