偶极EPR光谱定量蛋白质构象群的最新进展

IF 6.1 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current opinion in structural biology Pub Date : 2025-08-23 DOI:10.1016/j.sbi.2025.103139

Reza Dastvan , Stefan Stoll

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引用次数: 0

摘要

本文重点介绍了偶极电子顺磁共振（EPR）光谱学，包括双电子-电子共振（DEER）光谱学的最新应用和技术进展。这些方法提供了生物大分子中位点特异性自旋标记之间的纳米级距离分布。所得数据特别适合于量化多态和柔性蛋白质的构象集合体的结构和能量学。最近的应用跨越了广泛的系统，并伴随着自旋标记、氘化、细胞内测量、综合多技术方法和结合结构预测工具的新型计算建模方法的创新。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Recent advances in quantifying protein conformational ensembles with dipolar EPR spectroscopy

This perspective highlights recent applications and technological progress in dipolar electron paramagnetic resonance (EPR) spectroscopy, including double electron–electron resonance (DEER) spectroscopy. These methods provide nanoscale distance distributions between site-specific spin labels in biomacromolecules. The resulting data are particularly well suited for quantifying the structure and energetics of conformational ensembles of multi-state and flexible proteins. Recent applications span a wide range of systems and are accompanied by innovations in spin labeling, deuteration, in-cell measurements, integrative multi-technique approaches, and novel computational modeling methods combined with structure prediction tools.

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来源期刊

Current opinion in structural biology 生物-生化与分子生物学

CiteScore

12.20

自引率

2.90%

发文量

179

审稿时长

6-12 weeks

期刊介绍： Current Opinion in Structural Biology (COSB) aims to stimulate scientifically grounded, interdisciplinary, multi-scale debate and exchange of ideas. It contains polished, concise and timely reviews and opinions, with particular emphasis on those articles published in the past two years. In addition to describing recent trends, the authors are encouraged to give their subjective opinion of the topics discussed. In COSB, we help the reader by providing in a systematic manner: 1. The views of experts on current advances in their field in a clear and readable form. 2. Evaluations of the most interesting papers, annotated by experts, from the great wealth of original publications. [...] The subject of Structural Biology is divided into twelve themed sections, each of which is reviewed once a year. Each issue contains two sections, and the amount of space devoted to each section is related to its importance. -Folding and Binding- Nucleic acids and their protein complexes- Macromolecular Machines- Theory and Simulation- Sequences and Topology- New constructs and expression of proteins- Membranes- Engineering and Design- Carbohydrate-protein interactions and glycosylation- Biophysical and molecular biological methods- Multi-protein assemblies in signalling- Catalysis and Regulation