合生剂通过雌性激素受体调节雄性秋葵脑发育及生殖发育

IF 3.8 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Aamir Khan, Malabika Sikdar, Naveen Kango, Rashmi Srivastava
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引用次数: 0

摘要

脑-肠-微生物轴是连接胃肠道微生物与免疫、消化、生殖和神经系统的双向通讯。饲粮中添加鼠李糖乳杆菌、长双歧杆菌和甘露聚糖可能调节脑肠轴,并对雄性生殖生理产生影响。因此,本研究旨在探讨合生剂对未成熟雄性日本鹌鹑生殖和神经生理的影响。为此,在2周龄鹌鹑的日粮中分别添加1%鼠李糖乳杆菌(L)、长双歧杆菌(B)和甘露寡糖(M),并将其组合(LB和LBM)添加28天。氧化应激标志物包括丙二醛(MDA)、过氧化氢(H2O2)、高级氧蛋白产物(AOPPs);抗氧化酶(超氧化物歧化酶、过氧化氢酶)和谷胱甘肽(GSH)辅酶;测定大鼠脑内单胺氧化酶(MAO)和乙酰胆碱酯酶(AchE)活性。免疫荧光法分析脑内炎症标志物(IL-10、IL-1β和NF-κB)、凋亡标志物(caspase-3和caspase-7)和生殖方面促性腺激素释放激素(GnRH)和雌激素受体(ER-α和ER-β)。结果表明,饲粮中添加1%枸芪多糖或合成酶可显著增加海马区神经元数量和抗氧化酶(SOD和过氧化氢酶),降低MDA、H2O2和AOPP。合成物增加了IL-10的表达,并大幅降低了IL-1β和NF-κB的表达,从而限制了炎症和细胞凋亡(Caspase-3 & Caspase-7),增加了发育中的大脑中AchE和MAO的活性。我们的研究结果表明,合生剂干扰和促进神经元发育过程,并与生育有关,表现在大脑中GnRH, ER-α, ER-β的表达上调。综上所述,pro、pre、synbiotics联合使用具有协同效应,在鹌鹑大脑发育的神经免疫过程和生育过程中起调节作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Synbiotics as Modulators of Developing Brain for Reproductive Development Via Estrogen Receptors in Male Coturnix Coturnix Japonica

Synbiotics as Modulators of Developing Brain for Reproductive Development Via Estrogen Receptors in Male Coturnix Coturnix Japonica

Synbiotics as Modulators of Developing Brain for Reproductive Development Via Estrogen Receptors in Male Coturnix Coturnix Japonica

The brain-gut–microbiome axis represents a bidirectional communication linking the gastrointestinal microbiome with immunity, digestion, reproduction and nervous system. Dietary supplementation of Lactobacillus rhamnosus, Bifidobacterium longum and mannan-oligosaccharides may modulate the brain-gut-axis and also have an effect on male reproductive physiology. Therefore, the study aims to explore the reproductive and neuronal physiology potentials of synbiotics in immature male Japanese quails. To achieve this, two weeks old quails were supplemented with 1% Lactobacillus rhamnosus (L), Bifidobacterium longum (B) and mannan-oligosaccharides (M) in their diet, individually as well as in combinations (LB and LBM) for 28days. The oxidative stress markers including malondialdehyde (MDA), hydrogen peroxide (H2O2), advanced oxygen protein products (AOPPs); antioxidant enzymes {Superoxide Dismutase (SOD), Catalase} and coenzyme Glutathione (GSH); monoamine oxidase (MAO) and acetylcholinesterase (AchE) activity were estimated in the brain. Inflammatory biomarkers (IL-10, IL-1β and NF-κB), apoptotic biomarkers (caspase-3 and caspase-7) and for reproductive aspect viz. gonadotropin releasing hormone (GnRH) and estrogen receptors (ER-α and ER-β) were analyzed by immunofluorescence in the brain. The results revealed that dietary supplementation with 1% LBM or synbiotics significantly enhances the number of neurons in the hippocampal area, antioxidant enzymes (SOD and Catalase) while it reduces MDA, H2O2 and AOPP. Synbiotics increases IL-10 expression and a substantial reduction in IL-1β and NF-κB which limits inflammation as well as apoptosis (Caspase-3 & Caspase-7) with increased AchE, MAO activity in the developing brain. Our findings indicate that synbiotics interfere and enhance neuronal development processes and are associated with fertility manifested by upregulated expression of GnRH, ER-α, ER-β in brain. Overall, combined use of pro, pre, synbiotics have a synergistic effect and serve as modulators in neuro-immune processes and fertility in developing brain of quail.

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来源期刊
Neurochemical Research
Neurochemical Research 医学-神经科学
CiteScore
7.70
自引率
2.30%
发文量
320
审稿时长
6 months
期刊介绍: Neurochemical Research is devoted to the rapid publication of studies that use neurochemical methodology in research on nervous system structure and function. The journal publishes original reports of experimental and clinical research results, perceptive reviews of significant problem areas in the neurosciences, brief comments of a methodological or interpretive nature, and research summaries conducted by leading scientists whose works are not readily available in English.
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