David P Marancik, Christopher C Chadwick, Paul Fields, Charles A Manire, Terry M Norton, Justin R Perrault, Carolyn Cray
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There was a negative correlation between myeloid-related protein 126 and packed cell volume (<i>r</i> = -0.612, <i>P</i> = 0.005) and total solids (<i>r</i> = -0.497, <i>P</i> = 0.03) and between and Fulton's body condition index (<i>r</i> = -0.684, <i>P</i> = 0.001). Serum amyloid A showed a relatively high interquartile range (IQR) in moribund turtles and no significant correlations with clinical parameters. Myeloid-related protein 126 and cardiac troponin C were further evaluated by an enzyme-linked immunosorbent assay in a larger dataset of loggerhead, Kemp's ridley (<i>Lepidochelys kempii</i>) and green (<i>Chelonia mydas</i>) turtles. Plasma myeloid-related protein 126 was significantly lower in captive healthy (<i>n</i> = 7) and recovered (<i>n</i> = 23) turtles than in moribund (<i>n</i> = 25) and nesting green (<i>n</i> = 58) turtles. Green turtles with fibropapillomatosis (<i>n</i> = 10) were not significantly different from any group. Discriminating values between healthy/recovered and moribund turtles were 1.89 and 1.97 ng/ml by receiver operating characteristic and logistic regression analyses, respectively. Myeloid-related protein 126 decreased in successfully rehabilitated turtles (<i>n</i> = 18 turtles; <i>n</i> = 67 blood samples) and was negatively correlated with body condition score (<i>r</i> = -0.672, <i>P</i> < 0.001) and packed cell volume (<i>r</i> = -0.443, <i>P</i> = 0.009). Cardiac troponin C was significantly higher (<i>P</i> = 0.049) in moribund turtles (<i>n</i> = 16) compared to healthy/recovered turtles (<i>n</i> = 7) and in moribund samples (<i>n</i> = 11) compared to recovered samples (<i>n</i> = 11) in serially sampled turtles (<i>P</i> = 0.015), but was not predictive of health status. Myeloid-related protein 126 represents a strong biomarker candidate in sea turtles. Cardiac troponin C warrants further evaluation in a larger dataset and serum amyloid A requires examination of variables affecting pathophysiologic responses in sea turtles.</p>","PeriodicalId":54331,"journal":{"name":"Conservation Physiology","volume":"13 1","pages":"coaf061"},"PeriodicalIF":2.5000,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12350207/pdf/","citationCount":"0","resultStr":"{\"title\":\"Evaluation of myeloid-related protein 126, cardiac troponin C and serum amyloid A as potential plasma biomarkers of health and disease in sea turtles.\",\"authors\":\"David P Marancik, Christopher C Chadwick, Paul Fields, Charles A Manire, Terry M Norton, Justin R Perrault, Carolyn Cray\",\"doi\":\"10.1093/conphys/coaf061\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Sea turtle health assessments can be strengthened by developing conserved biomarkers that discriminate between healthy and diseased states. Serum amyloid A, myeloid-related protein 126 and cardiac troponin C (CTNC) were explored as potential biomarkers of sea turtle health. Plasma concentrations initially quantified using a targeted SPARCL™ assay significantly differed between moribund (<i>n</i> = 15) and recovered (<i>n</i> = 5) loggerhead turtles (<i>Caretta caretta</i>). There was a negative correlation between myeloid-related protein 126 and packed cell volume (<i>r</i> = -0.612, <i>P</i> = 0.005) and total solids (<i>r</i> = -0.497, <i>P</i> = 0.03) and between and Fulton's body condition index (<i>r</i> = -0.684, <i>P</i> = 0.001). Serum amyloid A showed a relatively high interquartile range (IQR) in moribund turtles and no significant correlations with clinical parameters. Myeloid-related protein 126 and cardiac troponin C were further evaluated by an enzyme-linked immunosorbent assay in a larger dataset of loggerhead, Kemp's ridley (<i>Lepidochelys kempii</i>) and green (<i>Chelonia mydas</i>) turtles. Plasma myeloid-related protein 126 was significantly lower in captive healthy (<i>n</i> = 7) and recovered (<i>n</i> = 23) turtles than in moribund (<i>n</i> = 25) and nesting green (<i>n</i> = 58) turtles. Green turtles with fibropapillomatosis (<i>n</i> = 10) were not significantly different from any group. Discriminating values between healthy/recovered and moribund turtles were 1.89 and 1.97 ng/ml by receiver operating characteristic and logistic regression analyses, respectively. Myeloid-related protein 126 decreased in successfully rehabilitated turtles (<i>n</i> = 18 turtles; <i>n</i> = 67 blood samples) and was negatively correlated with body condition score (<i>r</i> = -0.672, <i>P</i> < 0.001) and packed cell volume (<i>r</i> = -0.443, <i>P</i> = 0.009). Cardiac troponin C was significantly higher (<i>P</i> = 0.049) in moribund turtles (<i>n</i> = 16) compared to healthy/recovered turtles (<i>n</i> = 7) and in moribund samples (<i>n</i> = 11) compared to recovered samples (<i>n</i> = 11) in serially sampled turtles (<i>P</i> = 0.015), but was not predictive of health status. Myeloid-related protein 126 represents a strong biomarker candidate in sea turtles. 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引用次数: 0
摘要
海龟健康评估可以通过开发区分健康和患病状态的保守生物标志物来加强。探讨了血清淀粉样蛋白A、骨髓相关蛋白126和心肌肌钙蛋白C （CTNC）作为海龟健康的潜在生物标志物。最初使用靶向SPARCL™测定法定量的血浆浓度在死亡海龟（n = 15）和恢复海龟（n = 5）之间存在显著差异。髓系相关蛋白126与堆积细胞体积（r = -0.612, P = 0.005）和固体总量（r = -0.497, P = 0.03）呈负相关，与Fulton身体状况指数（r = -0.684, P = 0.001）呈负相关。死龟血清淀粉样蛋白A呈较高的四分位数范围（IQR），与临床参数无显著相关性。骨髓相关蛋白126和心肌肌钙蛋白C通过酶联免疫吸附试验在更大的红海龟、肯普雷氏海龟（Lepidochelys kempii）和绿海龟（Chelonia mydas）数据集中进一步评估。圈养健康海龟（n = 7）和康复海龟（n = 23）的血浆髓系相关蛋白126显著低于死亡海龟（n = 25）和筑巢绿海龟（n = 58）。绿海龟纤维乳头状瘤病（n = 10）与各组无显著差异。经受体工作特征分析和logistic回归分析，健康/康复海龟与死亡海龟的鉴别值分别为1.89和1.97 ng/ml。髓系相关蛋白126在成功康复的海龟（n = 18只海龟，n = 67份血液样本）中降低，并与身体状况评分呈负相关（r = -0.672, P r = -0.443, P = 0.009）。死亡海龟（n = 16）的心肌肌钙蛋白C显著高于健康/恢复海龟（n = 7），连续取样海龟的死亡样本（n = 11）的心肌肌钙蛋白C显著高于恢复样本（n = 11） （P = 0.015），但不能预测健康状况。髓系相关蛋白126是海龟强有力的生物标志物候选。心肌肌钙蛋白C需要在更大的数据集中进一步评估，血清淀粉样蛋白a需要检查影响海龟病理生理反应的变量。
Evaluation of myeloid-related protein 126, cardiac troponin C and serum amyloid A as potential plasma biomarkers of health and disease in sea turtles.
Sea turtle health assessments can be strengthened by developing conserved biomarkers that discriminate between healthy and diseased states. Serum amyloid A, myeloid-related protein 126 and cardiac troponin C (CTNC) were explored as potential biomarkers of sea turtle health. Plasma concentrations initially quantified using a targeted SPARCL™ assay significantly differed between moribund (n = 15) and recovered (n = 5) loggerhead turtles (Caretta caretta). There was a negative correlation between myeloid-related protein 126 and packed cell volume (r = -0.612, P = 0.005) and total solids (r = -0.497, P = 0.03) and between and Fulton's body condition index (r = -0.684, P = 0.001). Serum amyloid A showed a relatively high interquartile range (IQR) in moribund turtles and no significant correlations with clinical parameters. Myeloid-related protein 126 and cardiac troponin C were further evaluated by an enzyme-linked immunosorbent assay in a larger dataset of loggerhead, Kemp's ridley (Lepidochelys kempii) and green (Chelonia mydas) turtles. Plasma myeloid-related protein 126 was significantly lower in captive healthy (n = 7) and recovered (n = 23) turtles than in moribund (n = 25) and nesting green (n = 58) turtles. Green turtles with fibropapillomatosis (n = 10) were not significantly different from any group. Discriminating values between healthy/recovered and moribund turtles were 1.89 and 1.97 ng/ml by receiver operating characteristic and logistic regression analyses, respectively. Myeloid-related protein 126 decreased in successfully rehabilitated turtles (n = 18 turtles; n = 67 blood samples) and was negatively correlated with body condition score (r = -0.672, P < 0.001) and packed cell volume (r = -0.443, P = 0.009). Cardiac troponin C was significantly higher (P = 0.049) in moribund turtles (n = 16) compared to healthy/recovered turtles (n = 7) and in moribund samples (n = 11) compared to recovered samples (n = 11) in serially sampled turtles (P = 0.015), but was not predictive of health status. Myeloid-related protein 126 represents a strong biomarker candidate in sea turtles. Cardiac troponin C warrants further evaluation in a larger dataset and serum amyloid A requires examination of variables affecting pathophysiologic responses in sea turtles.
期刊介绍：
Conservation Physiology is an online only, fully open access journal published on behalf of the Society for Experimental Biology.
Biodiversity across the globe faces a growing number of threats associated with human activities. Conservation Physiology will publish research on all taxa (microbes, plants and animals) focused on understanding and predicting how organisms, populations, ecosystems and natural resources respond to environmental change and stressors. Physiology is considered in the broadest possible terms to include functional and mechanistic responses at all scales. We also welcome research towards developing and refining strategies to rebuild populations, restore ecosystems, inform conservation policy, and manage living resources. We define conservation physiology broadly and encourage potential authors to contact the editorial team if they have any questions regarding the remit of the journal.
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