偶联策略和连接体密度对精胺- acdex纳米颗粒-脾细胞偶联物性能的影响。

IF 3.1 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yuchen Su, Ruoyu Cheng, Bowei Du, Mai O. Soliman, Hongbo Zhang and Shiqi Wang
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引用次数: 0

摘要

生物医学工程中常用的方法是用纳米药物修饰细胞表面,形成纳米颗粒-细胞偶联物。尽管有各种可用的策略,有限的研究已经检查了共轭策略如何影响传递系统的效率和稳定性。在此,我们制备了具有蛋白质有效载荷的聚合物纳米颗粒(NPs),并用不同的连接剂对其进行修饰。这些NPs通过共价或静电相互作用与原代脾细胞结合,然后通过流式细胞术分析评估结合效率和稳定性。结果表明,静电相互作用更有效地实现了偶联,而共价相互作用提供了更大的稳定性。此外,纳米颗粒表面的连接剂密度也会影响其稳定性。体外培养3天后,连接体较少的NPs主要被脾细胞内化,而连接体较多的NPs部分留在细胞表面。总的来说,这项研究提供了纳米颗粒-细胞结合的基本见解,从而有助于治疗应用的活体药物设计和工程。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The impact of conjugation strategies and linker density on the performance of the Spermine-AcDex nanoparticle–splenocyte conjugate

The impact of conjugation strategies and linker density on the performance of the Spermine-AcDex nanoparticle–splenocyte conjugate

A common approach in living medicine engineering is modifying cell surfaces with nanomedicines to form nanoparticle–cell conjugates. Despite various available strategies, limited research has examined how conjugation strategies affect the efficiency and stability of the delivery systems. Herein, we prepared polymeric nanoparticles (NPs) with protein payloads and modified them with different linkers. These NPs were conjugated to primary splenocytes using either covalent or electrostatic interactions, followed by flow cytometry analysis to evaluate the conjugating efficiency and stability. The results demonstrated that electrostatic interactions were more effective in achieving conjugation, whereas covalent interactions provided greater stability. Furthermore, the linker density on the nanoparticle surface also affected the stability. After three days of in vitro culture, NPs with fewer linkers were predominantly internalized by the splenocytes, whereas those with more linkers partially remained on the cell surface. Overall, this study provides fundamental insights into nanoparticle–cell conjugation, thereby contributing to living medicine design and engineering for therapeutic applications.

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来源期刊
CiteScore
6.10
自引率
0.00%
发文量
128
审稿时长
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