利用人工蛋白脂化系统探讨棕榈酰化的意义。

IF 3.1 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Kazuki Uchida, Naofumi Shimokawa, Rie Wakabayashi, Shohei Shiomoto, Kiyohiro Toyofuku, Nozomu Ogushi, Masahiro Goto, Masaru Tanaka, Masahiro Takagi and Noriho Kamiya
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引用次数: 0

摘要

蛋白质脂化,特别是棕榈酰化(附着16碳脂肪酸),通过控制蛋白质在脂质膜上的功能来调节细胞行为。在本研究中,我们制备了一系列具有不同烷基链长(C8 ~ C22)的脂化绿色荧光蛋白(EGFP-lipids)。使用模型脂质膜和Jurkat(人类T淋巴细胞)细胞,我们评估了脂化如何影响这些蛋白-脂质结构的膜相互作用和囊泡运输。我们的研究结果表明,烷基链的延伸深刻地影响了egfp脂质的侧膜扩散和囊泡运输。只有模拟体内脂化的人工脂化蛋白在响应外部信号时才表现出细胞动力学,这突出了棕榈酸的自然选择对最大限度地发挥蛋白在脂膜上的功能的重要性。这一见解也可用于利用人工蛋白质脂化技术的膜工程,潜在地加速医学和工业发展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Exploring the significance of palmitoylation using an artificial protein lipidation system

Exploring the significance of palmitoylation using an artificial protein lipidation system

Protein lipidation, particularly palmitoylation (attachment of a 16-carbon fatty acid), regulates cellular behaviors by controlling protein function at lipid membranes. In this study, we prepared a series of lipidated green fluorescent proteins (“EGFP–lipids”) with various alkyl chain lengths (C8 to C22). Using model lipid membranes and Jurkat (human T lymphocyte) cells, we evaluated how lipidation affects the membrane interactions and vesicular transport from the membrane of these protein–lipid constructs. Our findings demonstrate that elongation of the alkyl chain profoundly affects both lateral membrane diffusion and vesicular transport of the EGFP–lipids. Only artificially lipidated proteins that mimic in vivo lipidation exhibited cellular dynamics in response to external signals, which highlights the significance of the natural selection of palmitic acid to maximize the function of proteins on lipid membranes. This insight can also be useful in membrane engineering using artificial protein lipidation techniques, potentially accelerating medical and industrial developments.

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来源期刊
CiteScore
6.10
自引率
0.00%
发文量
128
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