{"title":"桦树花粉提取物增强注射激活患者完成过敏原免疫治疗的b细胞记忆反应","authors":"Carolin Baum, Christian Möbs, Wolfgang Pfützner","doi":"10.1002/eji.70034","DOIUrl":null,"url":null,"abstract":"<p>Allergen immunotherapy (AIT) of patients with IgE-mediated allergy results in the synthesis of blocking IgG antibodies mediating allergen tolerance. However, as antibody concentrations wane after stopping AIT, tolerance may be lost. The impact of a single booster allergen application on B-cellular memory in AIT-treated birch pollen (BP)-allergic patients was investigated. Twenty-five patients with BP allergy who finished AIT 3–12 years ago received one allergen injection approximately 4 months prior to the next BP season. We determined peripheral Bet v 1-specific IgG-secreting cells (ASC) by ELISPOT analysis and BP-specific IgG, IgG4, and IgE serum antibodies by ImmunoCAP, and the allergen-blocking capacity of IgG/IgG4 antibodies by ELIFAB assay. Clinical responses were assessed by visual analog scales. Immunological findings were compared with the primary B cell response of 12 BP-allergic patients receiving conventional AIT. Bet v 1-specific ASC significantly increased 2–4 weeks after BP-injection, accompanied by enhanced levels of both BP-specific IgG/IgG4 antibodies and allergen-blocking serum activity. Compared with conventional up-dosing, a single booster vaccination led to a markedly stronger B cell response after 4 weeks. Allergen booster injection activates B-cellular memory associated with blocking IgG/IgG4 antibodies, pointing to reinforcement of allergen tolerance after completion of AIT.</p>","PeriodicalId":165,"journal":{"name":"European Journal of Immunology","volume":"55 8","pages":""},"PeriodicalIF":3.7000,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/eji.70034","citationCount":"0","resultStr":"{\"title\":\"Booster Injection with Birch Pollen Extract Activates B-Cellular Memory Responses in Patients Having Completed Allergen Immunotherapy\",\"authors\":\"Carolin Baum, Christian Möbs, Wolfgang Pfützner\",\"doi\":\"10.1002/eji.70034\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Allergen immunotherapy (AIT) of patients with IgE-mediated allergy results in the synthesis of blocking IgG antibodies mediating allergen tolerance. However, as antibody concentrations wane after stopping AIT, tolerance may be lost. The impact of a single booster allergen application on B-cellular memory in AIT-treated birch pollen (BP)-allergic patients was investigated. Twenty-five patients with BP allergy who finished AIT 3–12 years ago received one allergen injection approximately 4 months prior to the next BP season. We determined peripheral Bet v 1-specific IgG-secreting cells (ASC) by ELISPOT analysis and BP-specific IgG, IgG4, and IgE serum antibodies by ImmunoCAP, and the allergen-blocking capacity of IgG/IgG4 antibodies by ELIFAB assay. Clinical responses were assessed by visual analog scales. Immunological findings were compared with the primary B cell response of 12 BP-allergic patients receiving conventional AIT. Bet v 1-specific ASC significantly increased 2–4 weeks after BP-injection, accompanied by enhanced levels of both BP-specific IgG/IgG4 antibodies and allergen-blocking serum activity. Compared with conventional up-dosing, a single booster vaccination led to a markedly stronger B cell response after 4 weeks. Allergen booster injection activates B-cellular memory associated with blocking IgG/IgG4 antibodies, pointing to reinforcement of allergen tolerance after completion of AIT.</p>\",\"PeriodicalId\":165,\"journal\":{\"name\":\"European Journal of Immunology\",\"volume\":\"55 8\",\"pages\":\"\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2025-08-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/eji.70034\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"European Journal of Immunology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/eji.70034\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"European Journal of Immunology","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/eji.70034","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
对ige介导的过敏患者进行过敏原免疫治疗(AIT),可合成阻断介导过敏原耐受的IgG抗体。然而,当停止AIT后抗体浓度下降时,可能会失去耐受性。研究了单一强化过敏原应用对ait治疗桦树花粉(BP)过敏患者b细胞记忆的影响。25例3-12年前完成AIT的BP过敏患者在下一个BP季节前大约4个月接受一次过敏原注射。采用ELISPOT法检测外周血Bet v 1特异性IgG分泌细胞(ASC),采用ImmunoCAP法检测bp特异性IgG、IgG4和IgE血清抗体,采用ELIFAB法检测IgG/IgG4抗体的过敏原阻断能力。采用视觉模拟量表评估临床反应。免疫学结果与12例接受常规AIT治疗的bp过敏患者的原代B细胞反应进行比较。注射bp后2-4周,Bet v 1特异性ASC显著升高,bp特异性IgG/IgG4抗体水平和过敏原阻断血清活性均升高。与常规的增加剂量相比,单次加强接种在4周后导致明显更强的B细胞应答。过敏原增强剂注射激活与阻断IgG/IgG4抗体相关的b细胞记忆,表明在AIT完成后过敏原耐受性增强。
Booster Injection with Birch Pollen Extract Activates B-Cellular Memory Responses in Patients Having Completed Allergen Immunotherapy
Allergen immunotherapy (AIT) of patients with IgE-mediated allergy results in the synthesis of blocking IgG antibodies mediating allergen tolerance. However, as antibody concentrations wane after stopping AIT, tolerance may be lost. The impact of a single booster allergen application on B-cellular memory in AIT-treated birch pollen (BP)-allergic patients was investigated. Twenty-five patients with BP allergy who finished AIT 3–12 years ago received one allergen injection approximately 4 months prior to the next BP season. We determined peripheral Bet v 1-specific IgG-secreting cells (ASC) by ELISPOT analysis and BP-specific IgG, IgG4, and IgE serum antibodies by ImmunoCAP, and the allergen-blocking capacity of IgG/IgG4 antibodies by ELIFAB assay. Clinical responses were assessed by visual analog scales. Immunological findings were compared with the primary B cell response of 12 BP-allergic patients receiving conventional AIT. Bet v 1-specific ASC significantly increased 2–4 weeks after BP-injection, accompanied by enhanced levels of both BP-specific IgG/IgG4 antibodies and allergen-blocking serum activity. Compared with conventional up-dosing, a single booster vaccination led to a markedly stronger B cell response after 4 weeks. Allergen booster injection activates B-cellular memory associated with blocking IgG/IgG4 antibodies, pointing to reinforcement of allergen tolerance after completion of AIT.
期刊介绍:
The European Journal of Immunology (EJI) is an official journal of EFIS. Established in 1971, EJI continues to serve the needs of the global immunology community covering basic, translational and clinical research, ranging from adaptive and innate immunity through to vaccines and immunotherapy, cancer, autoimmunity, allergy and more. Mechanistic insights and thought-provoking immunological findings are of interest, as are studies using the latest omics technologies. We offer fast track review for competitive situations, including recently scooped papers, format free submission, transparent and fair peer review and more as detailed in our policies.