胶台丸通过调节Nrf2/ARE/HO-1信号通路改善APP/PS1小鼠认知功能障碍

IF 3.8 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Xin-Chen Wang, Guang-Liang Wu, Hai-Yan Cai, Chen-Liang Chu, Yan-Lu Liu, Han-Cheng Li, Jing Zheng, Huangbao Chen, Hao Lin, Shi-Yong Li, Xin Chen
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引用次数: 0

摘要

阿尔茨海默病(AD)是一种慢性、进行性和破坏性的神经退行性疾病,严重影响人类的记忆、智力和行为能力。焦台丸(JTW)是由黄连(coptis chinensis, CC)和肉桂(cinnamon, CIN)两种中药组成的经典配方,CC和CIN的比例为10:1。JTW具有促进认知功能、改善学习记忆功能的作用。但具体机理尚未系统研究。我们通过Morris水迷宫和y迷宫行为测试、聚合酶链反应(PCR)、检测试剂盒和ELISA、尼氏染色、western blotting验证JTW对APP/PS1小鼠认知功能的改善作用。通过行为学实验,JTW可以提高APP/PS1小鼠的学习能力和空间探索能力。Nissl染色及PCR检测BDNF、NGF、SYP均显示JTW能改善APP/PS1小鼠神经退行性病变。通过检测A β 1-40、A β 1-42、α -分泌酶、β -分泌酶和γ -分泌酶的活性,以及乙酰胆碱能标记酶Ach、AchE和ChAT的活性,表明JTW改善了APP/PS1模型小鼠的脑淀粉样变性和胆碱能神经系统。炎症因子和氧化应激指标检测显示,JTW可以抑制炎症因子和氧化应激的活性。Western Blotting检测Nrf2/ARE/HO-1通路,结果显示JTW可调节Nrf2/ARE/HO-1通路,改善APP/PS1小鼠的认知功能。JTW可增强APP/PS1小鼠Nrf2活性,调节Nrf2/ARE/HO-1通路,增加神经生长因子活性,改善胆碱能神经系统,抑制炎症因子和氧化应激,改善认知功能障碍。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Jiao-Tai-Wan Improves Cognitive Impairment by Regulating Nrf2/ARE/HO-1 Signaling Pathway in APP/PS1 Mice

Alzheimer’s disease (AD) is a chronic, progressive, and destructive neurodegenerative disorder that severely affects human memory, intelligence, and behavioral abilities. Jiao Tai Wan (JTW) is a classic formula composed of two traditional Chinese medicines, coptis chinensis (CC) and cinnamon (CIN), the ratio of CC and CIN is 10:1. JTW has the effects of promoting cognitive function, and improving learning and memory function. But the specific mechanism has not been systematically studied. We conducted Morris water maze and Y-maze behavioral tests, polymerase chain reaction (PCR), assay kit and ELISA, nissl’s staining, and western blotting to verified the improvement of cognitive function of JTW on APP/PS1 mice. Through behavioral experiments, JTW can improved the learning and spatial exploration abilities of APP/PS1 mice. Nissl’s staining and PCR detection of BDNF, NGF, and SYP showed that JTW can improved neurodegenerative lesions in APP/PS1 mice. By detecting the activities of A β 1–40, A β 1–42, α - secretase, β - secretase, and γ - secretase, as well as cholinergic labeling enzymes of Ach, AchE, and ChAT activities showed that JTW improved cerebral amyloidosis and cholinergic nervous system in APP/PS1 model mice. The detection of inflammatory factors and oxidative stress indicators revealed that JTW can inhibited the activity of inflammatory factors and oxidative stress. Western Blotting was used to detect the Nrf2/ARE/HO-1 pathway, and the result showed that JTW can regulate the Nrf2/ARE/HO-1 pathway to improving cognitive function of APP/PS1 mice. JTW enhanced the activity of Nrf2, regulated the Nrf2/ARE/HO-1 pathway, increased the activity of nerve growth factors, improved the cholinergic nervous system, inhibited inflammatory factors and oxidative stress, and ameliorated cognitive dysfunction in APP/PS1 mice.

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来源期刊
Neurochemical Research
Neurochemical Research 医学-神经科学
CiteScore
7.70
自引率
2.30%
发文量
320
审稿时长
6 months
期刊介绍: Neurochemical Research is devoted to the rapid publication of studies that use neurochemical methodology in research on nervous system structure and function. The journal publishes original reports of experimental and clinical research results, perceptive reviews of significant problem areas in the neurosciences, brief comments of a methodological or interpretive nature, and research summaries conducted by leading scientists whose works are not readily available in English.
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