以乳酸杆菌为基础的益生菌鸡尾酒通过改变肠道代谢抑制结肠炎相关的癌症。

IF 0.9 Q4 ONCOLOGY
International journal of clinical and experimental pathology Pub Date : 2025-07-15 eCollection Date: 2025-01-01 DOI:10.62347/USLL6631
Weiyi Wang, Ying Xu, Yimin Chu, Haiqin Zhang, Lu Zhou, Haijin Zhu, Ji Li, Zixu Zhang, Jinnian Cheng, Fengli Zhou, Daming Yang, Weisong Xu, Haixia Peng
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引用次数: 0

摘要

目的:肠道菌群失调是结直肠癌的重要发病机制。以乳酸杆菌为基础的益生菌鸡尾酒可通过缓解肠道生态失调来抑制结肠炎相关癌(CAC)。肠道微生物代谢物与结直肠癌的病因有关。然而,在CAC过程中,以乳酸杆菌为基础的益生菌鸡尾酒与肠道代谢改变之间的联系及其功能机制尚不清楚。方法:以偶氮甲烷/葡聚糖硫酸钠(AOM/DSS)诱导的CAC小鼠为研究对象,采用益生菌混合物预处理CAC小鼠,观察其保护作用。用C57BL/6小鼠结肠评估炎症和肿瘤发生。通过比较分析确定益生菌如何改变肠道代谢和基因表达。同时,分析肠道菌群变化。最后对肠道代谢、基因表达及肠道菌群进行综合分析。结果:益生菌预处理可减轻肠道炎症,抑制肿瘤的形成。益生菌预处理后,癌基因下调,抑癌基因上调。益生菌预处理诱导以乳酸杆菌为主的菌属增加,潜在致病菌Parasutterella、Helicobacter和Muribaculum减少,并影响涉及37种代谢途径的肠道代谢物的表达。乳酸杆菌相关的肠道代谢物变化涉及5种代谢途径——精氨酸和脯氨酸代谢、组氨酸代谢、嘧啶代谢、嘌呤代谢和酪氨酸代谢。结论:以乳酸杆菌为基础的益生菌鸡尾酒预处理通过干扰影响抑癌基因和癌基因表达的肠道代谢物来保护小鼠CAC。此外,乳杆菌影响了5种代谢途径,这是益生菌抗炎和抗肿瘤的重要机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Lactobacillus-based probiotic cocktail inhibits colitis-associated cancer by altering intestinal metabolism.

Objective: Dysbiosis of intestinal microbiome is an important colorectal cancer (CRC) pathogenetic mechanism. Lactobacillus-based probiotic cocktail could inhibit colitis-associated cancer (CAC) by alleviating intestinal dysbiosis. The intestinal microbial metabolites have been linked with CRC etiology. However, the link between Lactobacillus-based probiotic cocktail and the alteration of intestinal metabolism and their functional mechanisms during CAC process is still poorly understood.

Methods: For assessing protective effects of the probiotic cocktail, azomethanes/dextran sodium sulfate (AOM/DSS) induced CAC mice were pretreated with the probiotic cocktail. Colon of C57BL/6 mice were used to assess inflammation and tumorigenesis. Comparative analysis was performed for determining how the probiotic altered intestinal metabolism and gene expression. Meanwhile, intestinal microbiota alterations were analyzed. The concluding integrated analysis of intestinal metabolism and gene expression as well as intestinal microbiota was presented.

Results: Pretreatment with the probiotic alleviated intestinal inflammation and limited the formation of tumors. Oncogenes were down-regulated and cancer suppressor genes were up-regulated after probiotic pretreatment. Pretreatment with the probiotic induced a rise of Lactobacillus-dominated genera and a reduction of potential pathogenic bacteria Parasutterella, Helicobacter and Muribaculum, and affected expression of intestinal metabolites that involved 37 metabolic pathways. Lactobacillus-associated intestinal metabolite variations involve five metabolic pathways - arginine and proline metabolism, histidine metabolism, pyrimidine metabolism, purine metabolism, and tyrosine metabolism.

Conclusions: Pretreatment with Lactobacillus-based probiotic cocktail protected mice from CAC by interfering with intestinal metabolites that affected the cancer suppressor genes and oncogenes' expression. Furthermore, Lactobacillus affected five metabolite pathways, which was important mechanism for probiotic anti-inflammatory and anti-tumorigenesis.

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来源期刊
自引率
0.00%
发文量
42
审稿时长
1 months
期刊介绍: The International Journal of Clinical and Experimental Pathology (IJCEP, ISSN 1936-2625) is a peer reviewed, open access online journal. It was founded in 2008 by an international group of academic pathologists and scientists who are devoted to the scientific exploration of human disease and the rapid dissemination of original data. Unlike most other open access online journals, IJCEP will keep all the traditional features of paper print that we are all familiar with, such as continuous volume and issue numbers, as well as continuous page numbers to keep our warm feelings towards an academic journal. Unlike most other open access online journals, IJCEP will keep all the traditional features of paper print that we are all familiar with, such as continuous volume and issue numbers, as well as continuous page numbers to keep our warm feelings towards an academic journal.
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