Xiao-Li Wang, Jin-Chun Jiang, Jia-Ye Song, Jing-Yi Ni, Li Song, Jin-Zhang Xiao, Da Fu, Zi-Yu Chen, Yong-Feng Cao
{"title":"Hsa-miR-100-5p通过靶向非典型趋化因子受体3促进胃癌的发生进展。","authors":"Xiao-Li Wang, Jin-Chun Jiang, Jia-Ye Song, Jing-Yi Ni, Li Song, Jin-Zhang Xiao, Da Fu, Zi-Yu Chen, Yong-Feng Cao","doi":"10.62347/SBGT4820","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>Gastric cancer (GC) ranks as the fourth most prevalent malignancy globally and is a leading cause of cancer-related mortality. This study aims to comprehensively investigate the pathogenesis of gastric cancer and propose innovative strategies for early diagnosis.</p><p><strong>Methods: </strong>Leveraging data from The Cancer Genome Atlas (TCGA), we identified that hsa-miR-100-5p exhibits significantly reduced expression in gastric cancer tissues compared to normal tissues. Subsequently, the low expression levels and clinical significance of hsa-miR-100-5p were further validated through quantitative analysis in a cohort of 58 GC patients.</p><p><strong>Results: </strong>Treatment with an hsa-miR-100-5p mimic markedly inhibited the proliferation of BGC823 cells, whereas the introduction of an hsa-miR-100-5p inhibitor promoted cell proliferation. A dual-luciferase reporter assay confirmed that atypical chemokine receptor 3 (<i>ACKR3</i>) is a direct target gene of hsa-miR-100-5p. Furthermore, our findings revealed a significant negative correlation between <i>ACKR3</i> expression and hsa-miR-100-5p levels. Immunological correlation analysis suggested that <i>ACKR3</i> may play a critical role in modulating the tumor microenvironment within cancer-associated fibroblasts.</p><p><strong>Conclusion: </strong>Collectively, these results indicate that hsa-miR-100-5p may regulate <i>ACKR3</i> to enhance the migration and proliferation of GC cells, thereby contributing to the onset and progression of gastric cancer.</p>","PeriodicalId":13943,"journal":{"name":"International journal of clinical and experimental pathology","volume":"18 7","pages":"302-316"},"PeriodicalIF":0.9000,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12343462/pdf/","citationCount":"0","resultStr":"{\"title\":\"Hsa-miR-100-5p promotes the development and progression of gastric cancer through targeted atypical chemokine receptor 3.\",\"authors\":\"Xiao-Li Wang, Jin-Chun Jiang, Jia-Ye Song, Jing-Yi Ni, Li Song, Jin-Zhang Xiao, Da Fu, Zi-Yu Chen, Yong-Feng Cao\",\"doi\":\"10.62347/SBGT4820\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objectives: </strong>Gastric cancer (GC) ranks as the fourth most prevalent malignancy globally and is a leading cause of cancer-related mortality. This study aims to comprehensively investigate the pathogenesis of gastric cancer and propose innovative strategies for early diagnosis.</p><p><strong>Methods: </strong>Leveraging data from The Cancer Genome Atlas (TCGA), we identified that hsa-miR-100-5p exhibits significantly reduced expression in gastric cancer tissues compared to normal tissues. Subsequently, the low expression levels and clinical significance of hsa-miR-100-5p were further validated through quantitative analysis in a cohort of 58 GC patients.</p><p><strong>Results: </strong>Treatment with an hsa-miR-100-5p mimic markedly inhibited the proliferation of BGC823 cells, whereas the introduction of an hsa-miR-100-5p inhibitor promoted cell proliferation. A dual-luciferase reporter assay confirmed that atypical chemokine receptor 3 (<i>ACKR3</i>) is a direct target gene of hsa-miR-100-5p. Furthermore, our findings revealed a significant negative correlation between <i>ACKR3</i> expression and hsa-miR-100-5p levels. Immunological correlation analysis suggested that <i>ACKR3</i> may play a critical role in modulating the tumor microenvironment within cancer-associated fibroblasts.</p><p><strong>Conclusion: </strong>Collectively, these results indicate that hsa-miR-100-5p may regulate <i>ACKR3</i> to enhance the migration and proliferation of GC cells, thereby contributing to the onset and progression of gastric cancer.</p>\",\"PeriodicalId\":13943,\"journal\":{\"name\":\"International journal of clinical and experimental pathology\",\"volume\":\"18 7\",\"pages\":\"302-316\"},\"PeriodicalIF\":0.9000,\"publicationDate\":\"2025-07-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12343462/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International journal of clinical and experimental pathology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.62347/SBGT4820\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q4\",\"JCRName\":\"ONCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of clinical and experimental pathology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.62347/SBGT4820","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"ONCOLOGY","Score":null,"Total":0}
Hsa-miR-100-5p promotes the development and progression of gastric cancer through targeted atypical chemokine receptor 3.
Objectives: Gastric cancer (GC) ranks as the fourth most prevalent malignancy globally and is a leading cause of cancer-related mortality. This study aims to comprehensively investigate the pathogenesis of gastric cancer and propose innovative strategies for early diagnosis.
Methods: Leveraging data from The Cancer Genome Atlas (TCGA), we identified that hsa-miR-100-5p exhibits significantly reduced expression in gastric cancer tissues compared to normal tissues. Subsequently, the low expression levels and clinical significance of hsa-miR-100-5p were further validated through quantitative analysis in a cohort of 58 GC patients.
Results: Treatment with an hsa-miR-100-5p mimic markedly inhibited the proliferation of BGC823 cells, whereas the introduction of an hsa-miR-100-5p inhibitor promoted cell proliferation. A dual-luciferase reporter assay confirmed that atypical chemokine receptor 3 (ACKR3) is a direct target gene of hsa-miR-100-5p. Furthermore, our findings revealed a significant negative correlation between ACKR3 expression and hsa-miR-100-5p levels. Immunological correlation analysis suggested that ACKR3 may play a critical role in modulating the tumor microenvironment within cancer-associated fibroblasts.
Conclusion: Collectively, these results indicate that hsa-miR-100-5p may regulate ACKR3 to enhance the migration and proliferation of GC cells, thereby contributing to the onset and progression of gastric cancer.
期刊介绍:
The International Journal of Clinical and Experimental Pathology (IJCEP, ISSN 1936-2625) is a peer reviewed, open access online journal. It was founded in 2008 by an international group of academic pathologists and scientists who are devoted to the scientific exploration of human disease and the rapid dissemination of original data. Unlike most other open access online journals, IJCEP will keep all the traditional features of paper print that we are all familiar with, such as continuous volume and issue numbers, as well as continuous page numbers to keep our warm feelings towards an academic journal. Unlike most other open access online journals, IJCEP will keep all the traditional features of paper print that we are all familiar with, such as continuous volume and issue numbers, as well as continuous page numbers to keep our warm feelings towards an academic journal.