rs7975232基因中维生素D受体多态性与新冠肺炎易感性的关系

IF 1 4区 生物学 Q4 GENETICS & HEREDITY
Genetic testing and molecular biomarkers Pub Date : 2025-08-01 Epub Date: 2025-08-14 DOI:10.1177/19450265251366457
Zohreh Beiranvand, Ashkan Alamdary, Rasool Mohammadi, Mehdi Ajorloo
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引用次数: 0

摘要

背景:维生素D缺乏影响2019冠状病毒病(COVID-19)的致病性和严重程度,表明维生素D受体的多态性可能影响疾病易感性和结局。本研究旨在探讨rs7975232 SNP与严重急性呼吸综合征冠状病毒2(SARS-CoV-2)感染易感性的关系。材料和方法:本研究比较了霍拉马巴德Shohada-ye Ashayer医院的138名COVID-19患者和136名健康人。采用PCR-RFLP(聚合酶链反应-限制性片段长度多态性)法采集血样,确定基因型。PCR-RFLP方法的结果经测序证实。采用IBM SPSS和SNPStats软件比较基因型和等位基因频率,并进行优势比分析。结果:本研究结果未显示rs7975232多态性与COVID-19易感性之间存在显著关联(p = 0.58)。随后的分析显示,低淋巴细胞水平和CC基因型的个体对COVID-19的易感性增加(OR = 3.45, 95%CI: 1.18-10.11)。肌酐(p < 0.0001)、中性粒细胞(p = 0.02)和NLR(中性粒细胞与淋巴细胞比值)(p = 0.0015)与rs7975232之间存在显著相关性。在AA/AC基因型病例的比较中,ALT、CPK、肌酐、血糖和血红蛋白水平正常的个体表现出感染的可能性增加。在CC基因型病例中,中性粒细胞水平正常的个体感染COVID-19的几率降低。结论:目前的研究并未证明所检测的SNP与COVID-19之间存在显着关联。研究结果表明,各种实验室参数的标准水平影响疾病的可能性。涉及更大和更多样化的人群的其他研究可以产生更有效的结果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Association Between the Vitamin D Receptor Polymorphism in rs7975232 with the COVID-19 Susceptibility.

Background: Vitamin D deficiency influences the pathogenicity and severity of coronavirus disease 2019 (COVID-19), suggesting that polymorphisms in the vitamin D receptor may impact disease susceptibility and outcomes. This study aims to examine the relationship between the rs7975232 SNP and susceptibility to severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) infection. Materials and Methods: This study compared 138 COVID-19 patients with 136 healthy individuals at Shohada-ye Ashayer Hospital in Khorramabad. The PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) method was employed to ascertain the genotypes following the collection of blood samples. The results of PCR-RFLP method were confirmed by sequencing. IBM SPSS and SNPStats software were utilized to compare genotypes and allele frequencies, as well as to conduct odds ratio analysis. Results: This study's results did not demonstrate a significant association between the rs7975232 polymorphism and COVID-19 susceptibility (p = 0.58). Subsequent analysis revealed that individuals with low lymphocyte levels and the CC genotype exhibited increased susceptibility to COVID-19 (OR = 3.45, 95%CI: 1.18-10.11). A significant association was observed between creatinine (p < 0.0001), neutrophils (p = 0.02), and NLR (Neutrophil to Lymphocyte Ratio) (p = 0.0015) with rs7975232. In a comparison of AA/AC genotype cases, individuals with normal levels of ALT, CPK, creatinine, blood sugar, and hemoglobin exhibited an increased likelihood of infection. In CC genotype cases, individuals with normal neutrophil levels exhibited a reduced chance of COVID-19. Conclusion: The current study did not demonstrate a significant association between the examined SNP and COVID-19. The findings indicated that the standard levels of various laboratory parameters influence the likelihood of the disease. Additional studies involving larger and more diverse populations can yield more valid results.

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来源期刊
CiteScore
2.50
自引率
7.10%
发文量
63
审稿时长
1 months
期刊介绍: Genetic Testing and Molecular Biomarkers is the leading peer-reviewed journal covering all aspects of human genetic testing including molecular biomarkers. The Journal provides a forum for the development of new technology; the application of testing to decision making in an increasingly varied set of clinical situations; ethical, legal, social, and economic aspects of genetic testing; and issues concerning effective genetic counseling. This is the definitive resource for researchers, clinicians, and scientists who develop, perform, and interpret genetic tests and their results. Genetic Testing and Molecular Biomarkers coverage includes: -Diagnosis across the life span- Risk assessment- Carrier detection in individuals, couples, and populations- Novel methods and new instrumentation for genetic testing- Results of molecular, biochemical, and cytogenetic testing- Genetic counseling
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