Zohreh Beiranvand, Ashkan Alamdary, Rasool Mohammadi, Mehdi Ajorloo
{"title":"rs7975232基因中维生素D受体多态性与新冠肺炎易感性的关系","authors":"Zohreh Beiranvand, Ashkan Alamdary, Rasool Mohammadi, Mehdi Ajorloo","doi":"10.1177/19450265251366457","DOIUrl":null,"url":null,"abstract":"<p><p><b><i>Background:</i></b> Vitamin D deficiency influences the pathogenicity and severity of coronavirus disease 2019 (COVID-19), suggesting that polymorphisms in the vitamin D receptor may impact disease susceptibility and outcomes. This study aims to examine the relationship between the rs7975232 SNP and susceptibility to severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) infection. <b><i>Materials and Methods:</i></b> This study compared 138 COVID-19 patients with 136 healthy individuals at Shohada-ye Ashayer Hospital in Khorramabad. The PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) method was employed to ascertain the genotypes following the collection of blood samples. The results of PCR-RFLP method were confirmed by sequencing. IBM SPSS and SNPStats software were utilized to compare genotypes and allele frequencies, as well as to conduct odds ratio analysis. <b><i>Results:</i></b> This study's results did not demonstrate a significant association between the rs7975232 polymorphism and COVID-19 susceptibility (<i>p</i> = 0.58). Subsequent analysis revealed that individuals with low lymphocyte levels and the CC genotype exhibited increased susceptibility to COVID-19 (OR = 3.45, 95%CI: 1.18-10.11). A significant association was observed between creatinine (<i>p</i> < 0.0001), neutrophils (<i>p</i> = 0.02), and NLR (Neutrophil to Lymphocyte Ratio) (<i>p</i> = 0.0015) with rs7975232. In a comparison of AA/AC genotype cases, individuals with normal levels of ALT, CPK, creatinine, blood sugar, and hemoglobin exhibited an increased likelihood of infection. In CC genotype cases, individuals with normal neutrophil levels exhibited a reduced chance of COVID-19. <b><i>Conclusion:</i></b> The current study did not demonstrate a significant association between the examined SNP and COVID-19. The findings indicated that the standard levels of various laboratory parameters influence the likelihood of the disease. Additional studies involving larger and more diverse populations can yield more valid results.</p>","PeriodicalId":12603,"journal":{"name":"Genetic testing and molecular biomarkers","volume":" ","pages":"207-215"},"PeriodicalIF":1.0000,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Association Between the Vitamin D Receptor Polymorphism in rs7975232 with the COVID-19 Susceptibility.\",\"authors\":\"Zohreh Beiranvand, Ashkan Alamdary, Rasool Mohammadi, Mehdi Ajorloo\",\"doi\":\"10.1177/19450265251366457\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><b><i>Background:</i></b> Vitamin D deficiency influences the pathogenicity and severity of coronavirus disease 2019 (COVID-19), suggesting that polymorphisms in the vitamin D receptor may impact disease susceptibility and outcomes. This study aims to examine the relationship between the rs7975232 SNP and susceptibility to severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) infection. <b><i>Materials and Methods:</i></b> This study compared 138 COVID-19 patients with 136 healthy individuals at Shohada-ye Ashayer Hospital in Khorramabad. The PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) method was employed to ascertain the genotypes following the collection of blood samples. The results of PCR-RFLP method were confirmed by sequencing. IBM SPSS and SNPStats software were utilized to compare genotypes and allele frequencies, as well as to conduct odds ratio analysis. <b><i>Results:</i></b> This study's results did not demonstrate a significant association between the rs7975232 polymorphism and COVID-19 susceptibility (<i>p</i> = 0.58). Subsequent analysis revealed that individuals with low lymphocyte levels and the CC genotype exhibited increased susceptibility to COVID-19 (OR = 3.45, 95%CI: 1.18-10.11). A significant association was observed between creatinine (<i>p</i> < 0.0001), neutrophils (<i>p</i> = 0.02), and NLR (Neutrophil to Lymphocyte Ratio) (<i>p</i> = 0.0015) with rs7975232. In a comparison of AA/AC genotype cases, individuals with normal levels of ALT, CPK, creatinine, blood sugar, and hemoglobin exhibited an increased likelihood of infection. In CC genotype cases, individuals with normal neutrophil levels exhibited a reduced chance of COVID-19. <b><i>Conclusion:</i></b> The current study did not demonstrate a significant association between the examined SNP and COVID-19. The findings indicated that the standard levels of various laboratory parameters influence the likelihood of the disease. Additional studies involving larger and more diverse populations can yield more valid results.</p>\",\"PeriodicalId\":12603,\"journal\":{\"name\":\"Genetic testing and molecular biomarkers\",\"volume\":\" \",\"pages\":\"207-215\"},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2025-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Genetic testing and molecular biomarkers\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1177/19450265251366457\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/8/14 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genetic testing and molecular biomarkers","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1177/19450265251366457","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/8/14 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
Association Between the Vitamin D Receptor Polymorphism in rs7975232 with the COVID-19 Susceptibility.
Background: Vitamin D deficiency influences the pathogenicity and severity of coronavirus disease 2019 (COVID-19), suggesting that polymorphisms in the vitamin D receptor may impact disease susceptibility and outcomes. This study aims to examine the relationship between the rs7975232 SNP and susceptibility to severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) infection. Materials and Methods: This study compared 138 COVID-19 patients with 136 healthy individuals at Shohada-ye Ashayer Hospital in Khorramabad. The PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) method was employed to ascertain the genotypes following the collection of blood samples. The results of PCR-RFLP method were confirmed by sequencing. IBM SPSS and SNPStats software were utilized to compare genotypes and allele frequencies, as well as to conduct odds ratio analysis. Results: This study's results did not demonstrate a significant association between the rs7975232 polymorphism and COVID-19 susceptibility (p = 0.58). Subsequent analysis revealed that individuals with low lymphocyte levels and the CC genotype exhibited increased susceptibility to COVID-19 (OR = 3.45, 95%CI: 1.18-10.11). A significant association was observed between creatinine (p < 0.0001), neutrophils (p = 0.02), and NLR (Neutrophil to Lymphocyte Ratio) (p = 0.0015) with rs7975232. In a comparison of AA/AC genotype cases, individuals with normal levels of ALT, CPK, creatinine, blood sugar, and hemoglobin exhibited an increased likelihood of infection. In CC genotype cases, individuals with normal neutrophil levels exhibited a reduced chance of COVID-19. Conclusion: The current study did not demonstrate a significant association between the examined SNP and COVID-19. The findings indicated that the standard levels of various laboratory parameters influence the likelihood of the disease. Additional studies involving larger and more diverse populations can yield more valid results.
期刊介绍:
Genetic Testing and Molecular Biomarkers is the leading peer-reviewed journal covering all aspects of human genetic testing including molecular biomarkers. The Journal provides a forum for the development of new technology; the application of testing to decision making in an increasingly varied set of clinical situations; ethical, legal, social, and economic aspects of genetic testing; and issues concerning effective genetic counseling. This is the definitive resource for researchers, clinicians, and scientists who develop, perform, and interpret genetic tests and their results.
Genetic Testing and Molecular Biomarkers coverage includes:
-Diagnosis across the life span-
Risk assessment-
Carrier detection in individuals, couples, and populations-
Novel methods and new instrumentation for genetic testing-
Results of molecular, biochemical, and cytogenetic testing-
Genetic counseling