哺乳母猪拷贝数变异的全基因组检测及其与热应激生理解剖指标的关联研究

IF 1.9 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Letícia Fernanda de Oliveira, Renata Veroneze, Lorena Ferreira Benfica, André Campelo Araujo, Yijian Huang, Jay S Johnson, Luiz F Brito
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引用次数: 0

摘要

热应激反应指标是可遗传的复杂性状,具有多基因遗传。拷贝数变异(CNV)是重要的基因组结构变异,可能通过影响动物与热应激和抗病相关性状的表型变异而与气候适应有关。因此,本研究的主要目的是检测猪的CNV和CNV区域(CNVR),并探讨它们与哺乳期母猪HS反应生理解剖指标的关系。在969只动物中共检测到4184个常染色体基因组CNV(4012个缺失,172个重复)。通过合并至少有1 bp重叠的CNV来鉴定CNVR,从而鉴定出236个常染色体CNVR。考虑到所有记录、8:00时测量的阴道温度和毛发密度,关联分析导致鉴定出三个与耳皮温度显著相关的CNVR和一个与阴道温度显著相关的CNVR。11个基因携带CNVR并具有显著相关性。综上所述,在杂交母系猪中发现了多种CNV和CNVR,其中CNVR与泌乳母猪生理和解剖热应激反应指标显著相关。参与免疫和应激反应的候选基因与重要的CNVR重叠,表明它们可能有助于猪的气候适应能力。本研究结果有助于更好地了解哺乳母猪热应激反应的遗传背景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genome-Wide Detection of Copy Number Variation and Association Studies With Physiological and Anatomical Indicators of Heat Stress Response in Lactating Sows.

Indicators of heat stress response are heritable complex traits with polygenic inheritance. Copy number variations (CNV) are important genomic structural variations that may be linked to climatic adaptation by influencing the phenotypic variability of traits related to thermal stress and disease resistance in animals. Therefore, the primary objectives of this study were to detect CNV and CNV regions (CNVR) in pigs and explore their associations with physiological and anatomical indicators of HS response in lactating sows. A total of 4184 autosomal genome CNV (4012 deletions and 172 duplications) were detected in 969 animals. CNVR were identified by merging CNV with at least 1-bp overlap, which enabled the identification of 236 autosomal CNVR. The association analyses led to the identification of three CNVR significantly associated with ear skin temperature and one CNVR significantly associated with vaginal temperature considering all records, vaginal temperature measured at 8:00 h, and hair density. Eleven genes harboured the CNVR with significant associations. In summary, various CNV and CNVR were identified in crossbred maternal-line pigs, including CNVR significantly associated with physiological and anatomical heat stress response indicators in lactating sows. Candidate genes involved in immune and stress responses overlapped with the significant CNVR, suggesting that they may contribute to climatic resilience in pigs. The findings of this study contribute to better understanding the genetic background of heat stress response in lactating sows.

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来源期刊
Journal of Animal Breeding and Genetics
Journal of Animal Breeding and Genetics 农林科学-奶制品与动物科学
CiteScore
5.20
自引率
3.80%
发文量
58
审稿时长
12-24 weeks
期刊介绍: The Journal of Animal Breeding and Genetics publishes original articles by international scientists on genomic selection, and any other topic related to breeding programmes, selection, quantitative genetic, genomics, diversity and evolution of domestic animals. Researchers, teachers, and the animal breeding industry will find the reports of interest. Book reviews appear in many issues.
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