在含羰基化合物(糖)标记策略中,增强等压多重试剂促进n -聚糖的定量。

IF 2.7 2区 化学 Q2 BIOCHEMICAL RESEARCH METHODS
Jingwei Zhang, Zicong Wang, Yuan Liu, Henrik Zetterberg and Lingjun Li*, 
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引用次数: 0

摘要

聚糖是由多种单糖组成的复杂分子,具有多样的支链聚合物结构。由于多糖具有重要的生物学功能,基于质谱(MS)的定性和定量分析得到了广泛的研究。然而,在质谱分析中,传统的数据依赖采集(DDA)主要是在MS1扫描期间选择有限的丰富离子子集,以便在随后的MS2阶段进行碎片化。在本研究中,我们引入了一种先进的等压标签策略,该策略将大量与内容相关的样本标记为一个等压标签通道作为额外的增强通道。这一创新提高了等压多重试剂在定量糖组学中对含羰基化合物(SUGAR)进行标记的效率。值得注意的是,该方法显著改善了低丰度n -聚糖的表征,并能够检测到n -聚糖谱中细微的定量差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Boosting Quantification of N-Glycans by an Enhanced Isobaric Multiplex Reagents for Carbonyl-Containing Compound (SUGAR) Tagging Strategy

Boosting Quantification of N-Glycans by an Enhanced Isobaric Multiplex Reagents for Carbonyl-Containing Compound (SUGAR) Tagging Strategy

Glycans are complex molecules composed of various monosaccharides and exhibit diverse, branched polymer structures. Extensive research has been conducted on mass spectrometry (MS)-based qualitative and quantitative glycan analysis due to their critical biological functions. However, traditional data-dependent acquisition (DDA) in MS analysis primarily selects a limited subset of abundant ions during MS1 scans for fragmentation in subsequent MS2 stages. In this study, we introduce an advanced isobaric labeling strategy that incorporates a large amount of content-relevant sample labeled with one isobaric tag channel as an additional boosting channel. This innovation enhances the efficiency of isobaric multiplex reagents for carbonyl-containing compound (SUGAR) tagging in quantitative glycomics. Notably, this approach significantly improves the characterization of low-abundance N-glycans and enables the detection of subtle quantitative differences in N-glycan profiling.

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来源期刊
CiteScore
5.50
自引率
9.40%
发文量
257
审稿时长
1 months
期刊介绍: The Journal of the American Society for Mass Spectrometry presents research papers covering all aspects of mass spectrometry, incorporating coverage of fields of scientific inquiry in which mass spectrometry can play a role. Comprehensive in scope, the journal publishes papers on both fundamentals and applications of mass spectrometry. Fundamental subjects include instrumentation principles, design, and demonstration, structures and chemical properties of gas-phase ions, studies of thermodynamic properties, ion spectroscopy, chemical kinetics, mechanisms of ionization, theories of ion fragmentation, cluster ions, and potential energy surfaces. In addition to full papers, the journal offers Communications, Application Notes, and Accounts and Perspectives
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