氨基转移酶试剂中吡哆醛-5′-磷酸的补充:患者安全问题。

IF 3.7 2区 医学 Q1 MEDICAL LABORATORY TECHNOLOGY
Mauro Panteghini
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引用次数: 0

摘要

由于转氨酶是检测肝细胞损伤个体的第一级测试,因此对其测量的标准化是患者护理的优先需要。然而,获得一致的转氨酶结果强烈依赖于在转氨酶试剂中补充吡哆醛-5'-磷酸(P-5-P),因为这只能够报告可追溯到IFCC参考测量程序的临床样品的测量值,代表定义各自可测量酶量的最高计量水平。在使用的商业试剂中缺乏P-5-P添加是导致转氨酶结果不可接受的偏差的最常见原因,这与实验室和商业分析结果的一致性相反。然而,只有大约三分之一的医学实验室在其转氨酶测量中包括P-5-P。这表明缺乏对P-5-P补充的临床益处的认识,这不仅是分析偏好,而且代表了实验室在相关临床情况下提供高质量服务的优先事项。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Supplementation of pyridoxal-5'-phosphate in aminotransferase reagents: a matter of patient safety.

As aminotransferases represent the first-level tests to detect individuals with hepatocellular damage, standardization of their measurements is a priority need for patient care. The obtaining of the harmonization in aminotransferase results is however strongly dependent on supplementation of pyridoxal-5'-phosphate (P-5-P) in aminotransferase reagents as this exclusively enables reporting of measured values on clinical samples that are traceable to the IFCC reference measurements procedures, representing the highest metrological level defining the respective measurable enzyme quantity. The lack of P-5-P addition in the employed commercial reagents is the most frequent cause of unacceptably biased results of aminotransferases, which opposes to consistency of results across laboratories and commercial assays. Nevertheless, only about one-third of medical laboratories include P-5-P in their aminotransferase measurements. This demonstrates a lack of awareness of the clinical benefits of P-5-P supplementation, which is not just an analytical preference, but it represents a laboratory priority for providing a high-quality service in pertinent clinical situations.

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来源期刊
Clinical chemistry and laboratory medicine
Clinical chemistry and laboratory medicine 医学-医学实验技术
CiteScore
11.30
自引率
16.20%
发文量
306
审稿时长
3 months
期刊介绍: Clinical Chemistry and Laboratory Medicine (CCLM) publishes articles on novel teaching and training methods applicable to laboratory medicine. CCLM welcomes contributions on the progress in fundamental and applied research and cutting-edge clinical laboratory medicine. It is one of the leading journals in the field, with an impact factor over 3. CCLM is issued monthly, and it is published in print and electronically. CCLM is the official journal of the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) and publishes regularly EFLM recommendations and news. CCLM is the official journal of the National Societies from Austria (ÖGLMKC); Belgium (RBSLM); Germany (DGKL); Hungary (MLDT); Ireland (ACBI); Italy (SIBioC); Portugal (SPML); and Slovenia (SZKK); and it is affiliated to AACB (Australia) and SFBC (France). Topics: - clinical biochemistry - clinical genomics and molecular biology - clinical haematology and coagulation - clinical immunology and autoimmunity - clinical microbiology - drug monitoring and analysis - evaluation of diagnostic biomarkers - disease-oriented topics (cardiovascular disease, cancer diagnostics, diabetes) - new reagents, instrumentation and technologies - new methodologies - reference materials and methods - reference values and decision limits - quality and safety in laboratory medicine - translational laboratory medicine - clinical metrology Follow @cclm_degruyter on Twitter!
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