Anni Su, Jessica Tieng, Xueying S Xu, Richard P Tan, Yuchen Feng, Justin J-L Wong
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{"title":"m7G基因表达者METTL1和BUD23在肾透明细胞癌中过度表达具有致癌性","authors":"Anni Su, Jessica Tieng, Xueying S Xu, Richard P Tan, Yuchen Feng, Justin J-L Wong","doi":"10.1002/path.6453","DOIUrl":null,"url":null,"abstract":"<p>Emerging evidence shows that N7-methylguanosine (m<sup>7</sup>G) modification and its writers contribute to the development of diverse cancers. However, the role of m<sup>7</sup>G writers in kidney renal clear cell carcinoma (KIRC) remains unclear. In this study we show that the catalytic components of m<sup>7</sup>G writers, METTL1 and BUD23, are overexpressed in advanced KIRC and are associated with worse overall survival. Knockdown of <i>METTL1</i> or <i>BUD23</i> inhibited cell proliferation, colony formation, and migration in KIRC cell lines, indicating their oncogenic role in KIRC. Furthermore, we observed that <i>METTL1</i> and <i>BUD23</i> expression was negatively correlated with the expression of key tumor suppressor genes commonly dysregulated in KIRC. We observed METTL1-mediated m<sup>7</sup>G methylation in mRNAs expressed by these tumor suppressor genes, indicating that METTL1 may regulate these genes <i>via</i> m<sup>7</sup>G modification. Overall, our study highlights the oncogenic role of METTL1 and BUD23 in KIRC and their potential as prognostic biomarkers and therapeutic targets in KIRC. © 2025 The Author(s). <i>The Journal of Pathology</i> published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland.</p>","PeriodicalId":232,"journal":{"name":"The Journal of Pathology","volume":"267 1","pages":"1-9"},"PeriodicalIF":5.2000,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pathsocjournals.onlinelibrary.wiley.com/doi/epdf/10.1002/path.6453","citationCount":"0","resultStr":"{\"title\":\"Overexpression of m7G writers METTL1 and BUD23 confers oncogenicity in kidney renal clear cell carcinoma\",\"authors\":\"Anni Su, Jessica Tieng, Xueying S Xu, Richard P Tan, Yuchen Feng, Justin J-L Wong\",\"doi\":\"10.1002/path.6453\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Emerging evidence shows that N7-methylguanosine (m<sup>7</sup>G) modification and its writers contribute to the development of diverse cancers. However, the role of m<sup>7</sup>G writers in kidney renal clear cell carcinoma (KIRC) remains unclear. In this study we show that the catalytic components of m<sup>7</sup>G writers, METTL1 and BUD23, are overexpressed in advanced KIRC and are associated with worse overall survival. Knockdown of <i>METTL1</i> or <i>BUD23</i> inhibited cell proliferation, colony formation, and migration in KIRC cell lines, indicating their oncogenic role in KIRC. Furthermore, we observed that <i>METTL1</i> and <i>BUD23</i> expression was negatively correlated with the expression of key tumor suppressor genes commonly dysregulated in KIRC. We observed METTL1-mediated m<sup>7</sup>G methylation in mRNAs expressed by these tumor suppressor genes, indicating that METTL1 may regulate these genes <i>via</i> m<sup>7</sup>G modification. Overall, our study highlights the oncogenic role of METTL1 and BUD23 in KIRC and their potential as prognostic biomarkers and therapeutic targets in KIRC. © 2025 The Author(s). <i>The Journal of Pathology</i> published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland.</p>\",\"PeriodicalId\":232,\"journal\":{\"name\":\"The Journal of Pathology\",\"volume\":\"267 1\",\"pages\":\"1-9\"},\"PeriodicalIF\":5.2000,\"publicationDate\":\"2025-07-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://pathsocjournals.onlinelibrary.wiley.com/doi/epdf/10.1002/path.6453\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Journal of Pathology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://pathsocjournals.onlinelibrary.wiley.com/doi/10.1002/path.6453\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ONCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Journal of Pathology","FirstCategoryId":"3","ListUrlMain":"https://pathsocjournals.onlinelibrary.wiley.com/doi/10.1002/path.6453","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ONCOLOGY","Score":null,"Total":0}
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