Ângelo Antônio Oliveira Silva, Ítalo Ferreira de Leon, Felipe Silva Santos de Jesus, Daniel Dias Sampaio, André Luis Bartz Voigt, Natália Berne Pinheiro, Nathieli Bianchin Bottari, Leda Margarita Castaño-Barrios, Paola Alejandra Fiorani Celedon, Nilson Ivo Tonin Zanchin, Fabricio Klerynton Marchini, Maria Elisabeth Aires Berne, Fred Luciano Neves Santos
{"title":"克氏锥虫嵌合重组抗原对南美南美锥虫病的诊断价值","authors":"Ângelo Antônio Oliveira Silva, Ítalo Ferreira de Leon, Felipe Silva Santos de Jesus, Daniel Dias Sampaio, André Luis Bartz Voigt, Natália Berne Pinheiro, Nathieli Bianchin Bottari, Leda Margarita Castaño-Barrios, Paola Alejandra Fiorani Celedon, Nilson Ivo Tonin Zanchin, Fabricio Klerynton Marchini, Maria Elisabeth Aires Berne, Fred Luciano Neves Santos","doi":"10.1111/tmi.70019","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Chagas disease, caused by Trypanosoma cruzi, remains a significant public health challenge in Latin America, with diagnostic limitations hindering control efforts.</p><p><strong>Aim: </strong>Our study aimed to assess the diagnostic performance of four chimeric recombinant T. cruzi antigens (IBMP-8.1, IBMP-8.2, IBMP-8.3 and IBMP-8.4) in a highly endemic region in southern Brazil.</p><p><strong>Methods: </strong>Serum samples from 333 individuals residing in Canguçu, Rio Grande do Sul, were tested using an in-house ELISA platform. We assessed the sensitivity, specificity, accuracy, and diagnostic odds ratio (DOR) of individual and combined IBMP antigens through serial and parallel testing strategies.</p><p><strong>Results: </strong>All antigens exhibited 100% specificity and high accuracy (≥ 93.4%), with IBMP-8.1 and IBMP-8.4 showing the best overall performance (sensitivities of 80.0% and 76.7%; DORs of 109,136 and 89,659, respectively). Parallel testing using the combinations IBMP-8.1 + IBMP-8.3 and IBMP-8.1 + IBMP-8.4 achieved ≥ 95% sensitivity and > 99% accuracy.</p><p><strong>Conclusions: </strong>These findings support the use of IBMP chimeric antigens, particularly in combination, as reliable tools for Chagas disease diagnosis and surveillance, and highlight the importance of region-specific validation to ensure diagnostic equity in diverse endemic settings.</p>","PeriodicalId":23962,"journal":{"name":"Tropical Medicine & International Health","volume":" ","pages":"1107-1114"},"PeriodicalIF":2.3000,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12501557/pdf/","citationCount":"0","resultStr":"{\"title\":\"Diagnostic Performance of Chimeric Recombinant Antigens of Trypanosoma cruzi for Identifying Chagas Disease in Samples From Rio Grande do Sul.\",\"authors\":\"Ângelo Antônio Oliveira Silva, Ítalo Ferreira de Leon, Felipe Silva Santos de Jesus, Daniel Dias Sampaio, André Luis Bartz Voigt, Natália Berne Pinheiro, Nathieli Bianchin Bottari, Leda Margarita Castaño-Barrios, Paola Alejandra Fiorani Celedon, Nilson Ivo Tonin Zanchin, Fabricio Klerynton Marchini, Maria Elisabeth Aires Berne, Fred Luciano Neves Santos\",\"doi\":\"10.1111/tmi.70019\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Chagas disease, caused by Trypanosoma cruzi, remains a significant public health challenge in Latin America, with diagnostic limitations hindering control efforts.</p><p><strong>Aim: </strong>Our study aimed to assess the diagnostic performance of four chimeric recombinant T. cruzi antigens (IBMP-8.1, IBMP-8.2, IBMP-8.3 and IBMP-8.4) in a highly endemic region in southern Brazil.</p><p><strong>Methods: </strong>Serum samples from 333 individuals residing in Canguçu, Rio Grande do Sul, were tested using an in-house ELISA platform. We assessed the sensitivity, specificity, accuracy, and diagnostic odds ratio (DOR) of individual and combined IBMP antigens through serial and parallel testing strategies.</p><p><strong>Results: </strong>All antigens exhibited 100% specificity and high accuracy (≥ 93.4%), with IBMP-8.1 and IBMP-8.4 showing the best overall performance (sensitivities of 80.0% and 76.7%; DORs of 109,136 and 89,659, respectively). Parallel testing using the combinations IBMP-8.1 + IBMP-8.3 and IBMP-8.1 + IBMP-8.4 achieved ≥ 95% sensitivity and > 99% accuracy.</p><p><strong>Conclusions: </strong>These findings support the use of IBMP chimeric antigens, particularly in combination, as reliable tools for Chagas disease diagnosis and surveillance, and highlight the importance of region-specific validation to ensure diagnostic equity in diverse endemic settings.</p>\",\"PeriodicalId\":23962,\"journal\":{\"name\":\"Tropical Medicine & International Health\",\"volume\":\" \",\"pages\":\"1107-1114\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2025-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12501557/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Tropical Medicine & International Health\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1111/tmi.70019\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/8/7 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tropical Medicine & International Health","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1111/tmi.70019","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/8/7 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH","Score":null,"Total":0}
Diagnostic Performance of Chimeric Recombinant Antigens of Trypanosoma cruzi for Identifying Chagas Disease in Samples From Rio Grande do Sul.
Background: Chagas disease, caused by Trypanosoma cruzi, remains a significant public health challenge in Latin America, with diagnostic limitations hindering control efforts.
Aim: Our study aimed to assess the diagnostic performance of four chimeric recombinant T. cruzi antigens (IBMP-8.1, IBMP-8.2, IBMP-8.3 and IBMP-8.4) in a highly endemic region in southern Brazil.
Methods: Serum samples from 333 individuals residing in Canguçu, Rio Grande do Sul, were tested using an in-house ELISA platform. We assessed the sensitivity, specificity, accuracy, and diagnostic odds ratio (DOR) of individual and combined IBMP antigens through serial and parallel testing strategies.
Results: All antigens exhibited 100% specificity and high accuracy (≥ 93.4%), with IBMP-8.1 and IBMP-8.4 showing the best overall performance (sensitivities of 80.0% and 76.7%; DORs of 109,136 and 89,659, respectively). Parallel testing using the combinations IBMP-8.1 + IBMP-8.3 and IBMP-8.1 + IBMP-8.4 achieved ≥ 95% sensitivity and > 99% accuracy.
Conclusions: These findings support the use of IBMP chimeric antigens, particularly in combination, as reliable tools for Chagas disease diagnosis and surveillance, and highlight the importance of region-specific validation to ensure diagnostic equity in diverse endemic settings.
期刊介绍：
Tropical Medicine & International Health is published on behalf of the London School of Hygiene and Tropical Medicine, Swiss Tropical and Public Health Institute, Foundation Tropical Medicine and International Health, Belgian Institute of Tropical Medicine and Bernhard-Nocht-Institute for Tropical Medicine. Tropical Medicine & International Health is the official journal of the Federation of European Societies for Tropical Medicine and International Health (FESTMIH).
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