{"title":"纳米包封聚合东莨菪素抗HT-29结肠癌细胞的多方面研究。","authors":"Kunnathur Murugesan Sakthivel, Rajan Radha Rasmi, Kalavathy Murugan Kumar, Kavitha Thangavelu, Loganathan Chandramani Priya Dharshini, Anitha Nagarajan, Balasubramanian Ramesh","doi":"10.1002/bdd.70010","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>In the sphere of medical research, nanoencapsulated polymeric drugs in biological circumstances are an intriguing issue for therapeutical diagnosis. The nanoprecipitation approach was employed in this study to synthesis the nanoencapsulated polymeric scopoletin (NEP-Sc) and tested its effect against the HT-29 cell line (human colorectal adenocarcinoma cells). Further, the synthesized NEP-Sc was then characterized by ultra-visible spectroscopy (UV–Vis), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM) with energy dispersive X-ray analysis (EDAX), and dynamic light scattering (DLS) analysis. All the analysis confirmed the effective synthesis of NEP-Sc. The synthesized NEP-Sc was validated as anticancer drug through the conventional MTT assay, neutral red uptake assay, apoptosis assay using AO/EB and lactate dehydrogenase assay and trypan blue assay against HT-29 cells. The docking studies of scopoletin with multiple genes were also carried out using the PyRx—virtual screening tool. The findings indicated that increase in concentration of NEP-Sc exhibited dose dependent action of cell death against the HT-29 colon cancer cells. To summarize, the observed results of anticancer and computational modeling studies underscores the therapeutic potential of NEP-Sc in treating colon cancer.</p>\n </div>","PeriodicalId":8865,"journal":{"name":"Biopharmaceutics & Drug Disposition","volume":"46 3","pages":"127-144"},"PeriodicalIF":2.0000,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Multifaceted Evaluation of Nano Encapsulated Polymeric Scopoletin Against HT-29 Colon Cancer Cells\",\"authors\":\"Kunnathur Murugesan Sakthivel, Rajan Radha Rasmi, Kalavathy Murugan Kumar, Kavitha Thangavelu, Loganathan Chandramani Priya Dharshini, Anitha Nagarajan, Balasubramanian Ramesh\",\"doi\":\"10.1002/bdd.70010\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>In the sphere of medical research, nanoencapsulated polymeric drugs in biological circumstances are an intriguing issue for therapeutical diagnosis. The nanoprecipitation approach was employed in this study to synthesis the nanoencapsulated polymeric scopoletin (NEP-Sc) and tested its effect against the HT-29 cell line (human colorectal adenocarcinoma cells). Further, the synthesized NEP-Sc was then characterized by ultra-visible spectroscopy (UV–Vis), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM) with energy dispersive X-ray analysis (EDAX), and dynamic light scattering (DLS) analysis. All the analysis confirmed the effective synthesis of NEP-Sc. The synthesized NEP-Sc was validated as anticancer drug through the conventional MTT assay, neutral red uptake assay, apoptosis assay using AO/EB and lactate dehydrogenase assay and trypan blue assay against HT-29 cells. The docking studies of scopoletin with multiple genes were also carried out using the PyRx—virtual screening tool. The findings indicated that increase in concentration of NEP-Sc exhibited dose dependent action of cell death against the HT-29 colon cancer cells. To summarize, the observed results of anticancer and computational modeling studies underscores the therapeutic potential of NEP-Sc in treating colon cancer.</p>\\n </div>\",\"PeriodicalId\":8865,\"journal\":{\"name\":\"Biopharmaceutics & Drug Disposition\",\"volume\":\"46 3\",\"pages\":\"127-144\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-08-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biopharmaceutics & Drug Disposition\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/bdd.70010\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"PHARMACOLOGY & PHARMACY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biopharmaceutics & Drug Disposition","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/bdd.70010","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
Multifaceted Evaluation of Nano Encapsulated Polymeric Scopoletin Against HT-29 Colon Cancer Cells
In the sphere of medical research, nanoencapsulated polymeric drugs in biological circumstances are an intriguing issue for therapeutical diagnosis. The nanoprecipitation approach was employed in this study to synthesis the nanoencapsulated polymeric scopoletin (NEP-Sc) and tested its effect against the HT-29 cell line (human colorectal adenocarcinoma cells). Further, the synthesized NEP-Sc was then characterized by ultra-visible spectroscopy (UV–Vis), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM) with energy dispersive X-ray analysis (EDAX), and dynamic light scattering (DLS) analysis. All the analysis confirmed the effective synthesis of NEP-Sc. The synthesized NEP-Sc was validated as anticancer drug through the conventional MTT assay, neutral red uptake assay, apoptosis assay using AO/EB and lactate dehydrogenase assay and trypan blue assay against HT-29 cells. The docking studies of scopoletin with multiple genes were also carried out using the PyRx—virtual screening tool. The findings indicated that increase in concentration of NEP-Sc exhibited dose dependent action of cell death against the HT-29 colon cancer cells. To summarize, the observed results of anticancer and computational modeling studies underscores the therapeutic potential of NEP-Sc in treating colon cancer.
期刊介绍：
Biopharmaceutics & Drug Dispositionpublishes original review articles, short communications, and reports in biopharmaceutics, drug disposition, pharmacokinetics and pharmacodynamics, especially those that have a direct relation to the drug discovery/development and the therapeutic use of drugs. These includes:
- animal and human pharmacological studies that focus on therapeutic response. pharmacodynamics, and toxicity related to plasma and tissue concentrations of drugs and their metabolites,
- in vitro and in vivo drug absorption, distribution, metabolism, transport, and excretion studies that facilitate investigations related to the use of drugs in man
- studies on membrane transport and enzymes, including their regulation and the impact of pharmacogenomics on drug absorption and disposition,
- simulation and modeling in drug discovery and development
- theoretical treatises
- includes themed issues and reviews
and exclude manuscripts on
- bioavailability studies reporting only on simple PK parameters such as Cmax, tmax and t1/2 without mechanistic interpretation
- analytical methods
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
