基于社区微生物源追踪方法的城市化海湾贝类养殖区粪便污染模式时空变化评估

IF 3 4区 环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES
Jiannan Lin, Hongxia Ming, Dongwei Li, Jiajie Li, Zhongzhi Chen, Ziqi Chen, Tingting Shi, Jie Su, Jingfeng Fan
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引用次数: 0

摘要

粪便污染对海洋贝类养殖区的公众健康和水产养殖构成重大风险。本研究采用粪便指示菌定量和16S rRNA基因测序技术对塔河湾中微生物污染进行了评价。在过去一年里,我们每月从贝类养殖区内的六个地点和一个用于沐浴的海滩地区的海水中采集样本。此外,沿着塔河湾海岸线收集了各种环境和生物样本,包括三个排水口的污水、海滩沙子、雨水径流以及人类、狗和鸟类的粪便样本。源代码贡献是使用SourceTracker程序确定的。在76%的海水样本中检测到肠球菌,表明广泛存在粪便污染,来自码头、海鲜加工厂的污水排放和邻近城镇的生活污水分别占污染的39%、11%和28%。空间分析显示,靠近这些排放物的近岸地区,Arcobacter和thiiothrix的丰度增加。虽然来自人类、狗、鸟类和海滩沙子的贡献一般较小,但在浴场观察到人类粪便微生物水平增加。微生物群落组成的季节变化主要由温度和盐度驱动,关键分类群,包括弧菌、海洋细菌和聚球菌,表现出环境敏感性。降水和潮汐波动进一步调节了细菌的分布,而亚硫酸盐杆菌和白毛菌等属的优势表明营养富集和潜在的富营养化。研究结果表明,基于社区的微生物源追踪是监测贝类养殖区粪便污染的有效工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Assessment of spatial and temporal variability in fecal contamination patterns within urbanized bay shellfish growing area utilizing community-based microbial source tracking approaches.

Fecal contamination poses substantial risks to public health and aquaculture in marine shellfish growing areas. This study evaluated microbial contamination in Tahe Bay using fecal indicator bacteria quantification and 16S rRNA gene sequencing. Samples were collected over 1 year on a monthly basis from seawater at six sites within the shellfish growing area and one site at a beach area used for bathing. Additionally, various environmental and biological samples were collected along the Tahe Bay coastline, including sewage from three outfalls, beach sand, rainwater runoff, and fecal samples from humans, dogs, and birds. Source contributions were determined using the SourceTracker program. Enterococci were detected in 76% of seawater samples, indicating widespread fecal contamination, with sewage outfalls originating from a marina, seafood-processing plant, and domestic sewage of an adjacent town contributing 39, 11, and 28% of the contamination. Spatial analysis revealed increased abundances of Arcobacter and Thiothrix nearshore, in close proximity to these outfalls. Although contributions from humans, dogs, birds, and beach sand were generally minor, increased levels of human fecal microbes were observed at the bathing beach site. Seasonal shifts in microbial community composition were primarily driven by temperature and salinity, with key taxa, including Vibrio, Marinobacterium, and Synechococcus, exhibiting environmental sensitivity. Precipitation and tidal fluctuations further modulated bacterial distribution, while the predominance of genera such as Sulfitobacter and Leucothrix indicated nutrient enrichment and potential eutrophication. The findings demonstrate that community-based microbial source tracking is an effective tool for monitoring fecal pollution in shellfish growing areas.

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来源期刊
Environmental Monitoring and Assessment
Environmental Monitoring and Assessment 环境科学-环境科学
CiteScore
4.70
自引率
6.70%
发文量
1000
审稿时长
7.3 months
期刊介绍: Environmental Monitoring and Assessment emphasizes technical developments and data arising from environmental monitoring and assessment, the use of scientific principles in the design of monitoring systems at the local, regional and global scales, and the use of monitoring data in assessing the consequences of natural resource management actions and pollution risks to man and the environment.
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