大鼠不完全性脊髓损伤模型瘫痪恢复过程中的步态分析

IF 3.9 3区医学 Q1 ORTHOPEDICS

JOR Spine Pub Date : 2025-08-06 DOI:10.1002/jsp2.70102

Misa Toyota, Shion Masuda, Daiki Nohara, Satoru Oba, Mayu Tomomitsu, Momoko Nagai-Tanima, Tomoki Aoyama

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摘要

在本研究中，我们旨在建立不完全脊髓损伤大鼠模型，并确定瘫痪恢复过程中肌肉张力与步态特征之间的关系。这种必要性源于对脊髓损伤康复发展中运动功能动力学动物模型的需求。方法38周龄雄性Sprague-Dawley大鼠随机分为假手术组和脊髓损伤组。分别于术后第3、7、14天进行三维步态分析、Hoffman反射、Basso-Beatie-Bresnahan评分、肌肉湿重和组织学评估。结果不完全脊髓损伤模型术后第14天出现麻痹恢复。与地面接触时，脊髓损伤组术后第3天踝关节底屈曲角度高于Sham组；然而，术后第14天减少。然而，在术后第3天和第14天，足离期的踝关节跖屈曲角度明显升高。脊髓损伤组大鼠踝关节外展角随时间明显增高，各时间点均高于Sham组。Hoffmann反射结果显示脊髓损伤组术后第3天肌肉张力明显增高，且随时间延长而增高。肌张力变化对模型的步态有显著影响。结论该模型为研究痉挛性步态和步态变化与麻痹改善的关系提供了有价值的工具。

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Gait Analysis During Paralysis Recovery in a Rat Incomplete Spinal Cord Injury Model

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Gait Analysis During Paralysis Recovery in a Rat Incomplete Spinal Cord Injury Model

Background

In this study, we aimed to create a rat model of incomplete spinal cord injury and to determine the relationship between muscle tone and gait characteristics during recovery from paralysis. This necessity stems from the need for animal models with motor function dynamics in rehabilitation development for spinal cord injury.

Methods

Thirty-eight-week-old male Sprague-Dawley rats were divided randomly into two groups: Sham and spinal cord injury groups. Three-dimensional gait analysis, Hoffman reflex, Basso-Beatie-Bresnahan score, muscle wet weight, and histological assessment were performed on postoperative days 3, 7, and 14, respectively.

Results

The incomplete spinal cord injury model showed paralysis recovery over time at postoperative day 14. At ground contact, the ankle plantar flexion angle was higher in the spinal cord injury group than in the Sham group on postoperative day 3; however, it reduced on postoperative day 14. Nevertheless, the ankle plantar flexion angle on the foot-off phase was significantly higher on postoperative days 3 and 14. The ankle abduction angle in the spinal cord injury group significantly increased over time and was higher than in the Sham group at all time points. Hoffmann reflex results showed that muscle tone was significantly higher in the spinal cord injury group on postoperative day 3 and increased over time. The model's gait was significantly affected by muscle tone changes.