利用同位素标记的内部标准解锁空间代谢组学

IF 20.8 1区医学 Q1 ENDOCRINOLOGY & METABOLISM

Nature metabolism Pub Date : 2025-08-05 DOI:10.1038/s42255-025-01341-7

引用次数: 0

摘要

我们提出了一种具有成本效益的空间代谢组学规范化策略，使用统一的13c标记酵母提取物，解决了传统方法的局限性和水溶性代谢物的物理化学复杂性。我们的方法优于标准的规范化策略，并揭示了迄今为止未被认识的脑卒中后皮层代谢重塑，证明了其适用性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Unlocking spatial metabolomics with isotopically labelled internal standards

查看原文本刊更多论文

Unlocking spatial metabolomics with isotopically labelled internal standards

We present a cost-effective normalization strategy for spatial metabolomics using uniformly 13C-labelled yeast extract, which addresses limitations of conventional methods and the physico-chemical complexity of water-soluble metabolites. Our approach outperforms standard normalization strategies and reveals hitherto unrecognized metabolic remodelling in the cortex after stroke, demonstrating its applicability.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Nature metabolism ENDOCRINOLOGY & METABOLISM-

CiteScore

27.50

自引率

2.40%

发文量

170

期刊介绍： Nature Metabolism is a peer-reviewed scientific journal that covers a broad range of topics in metabolism research. It aims to advance the understanding of metabolic and homeostatic processes at a cellular and physiological level. The journal publishes research from various fields, including fundamental cell biology, basic biomedical and translational research, and integrative physiology. It focuses on how cellular metabolism affects cellular function, the physiology and homeostasis of organs and tissues, and the regulation of organismal energy homeostasis. It also investigates the molecular pathophysiology of metabolic diseases such as diabetes and obesity, as well as their treatment. Nature Metabolism follows the standards of other Nature-branded journals, with a dedicated team of professional editors, rigorous peer-review process, high standards of copy-editing and production, swift publication, and editorial independence. The journal has a high impact factor, has a certain influence in the international area, and is deeply concerned and cited by the majority of scholars.