基于铁氧化还原蛋白- nadp +氧化还原酶的通用电极使酶生物燃料电池具有广泛的底物谱

IF 3.1 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Biotechnology Journal Pub Date : 2025-08-04 DOI:10.1002/biot.70090

Hailing Dai, Guoqiang Chen, Jingjiu Mu, Afreen Shagufta, Lijuan Liu, Fan Wang, Sheng Dong, Xiao Men, Lei Wang, Haibo Zhang

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引用次数: 0

摘要

由于电极的酶特异性，酶生物燃料电池面临底物限制。采用细菌纤维素（BC）、碳纳米管（CNTs）和银纳米线（AgNWs）固定铁氧化还原蛋白- nadp +氧化还原酶（FNR），设计了通用电极。电极偶联nadph依赖性苹果酸酶或葡萄糖脱氢酶，分别利用苹果酸和葡萄糖发电。开路电压分别达到79.36和75.8 mV， 12 h后丙酮酸盐和葡萄糖酸盐的积累量分别达到0.30和0.25 mM。这种策略使得电子可以通过NADPH从不同的底物转移。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Universal Electrode Based on Ferredoxin-NADP+ Oxidoreductase Enables Enzymatic Biofuel Cells With Broad Substrate Spectrum

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Universal Electrode Based on Ferredoxin-NADP+ Oxidoreductase Enables Enzymatic Biofuel Cells With Broad Substrate Spectrum

Enzymatic biofuel cells face substrate limitations due to enzyme specificity of the electrode. A universal electrode was designed by immobilizing ferredoxin-NADP⁺ oxidoreductase (FNR) with bacterial cellulose (BC), carbon nanotubes (CNTs), and silver nanowires (AgNWs). The electrode coupled with NADPH-dependent malic enzyme or glucose dehydrogenase generated electricity using malic acid and glucose, respectively. The open-circuit voltage reached 79.36 and 75.8 mV, respectively, and the accumulation of pyruvate and gluconate reached 0.30 and 0.25 mM, respectively, after 12 h. This strategy enables electron transfer from diverse substrates via NADPH.

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来源期刊

Biotechnology Journal Biochemistry, Genetics and Molecular Biology-Molecular Medicine

CiteScore

8.90

自引率

2.10%

发文量

123

审稿时长

1.5 months

期刊介绍： Biotechnology Journal (2019 Journal Citation Reports: 3.543) is fully comprehensive in its scope and publishes strictly peer-reviewed papers covering novel aspects and methods in all areas of biotechnology. Some issues are devoted to a special topic, providing the latest information on the most crucial areas of research and technological advances. In addition to these special issues, the journal welcomes unsolicited submissions for primary research articles, such as Research Articles, Rapid Communications and Biotech Methods. BTJ also welcomes proposals of Review Articles - please send in a brief outline of the article and the senior author''s CV to the editorial office. BTJ promotes a special emphasis on: Systems Biotechnology Synthetic Biology and Metabolic Engineering Nanobiotechnology and Biomaterials Tissue engineering, Regenerative Medicine and Stem cells Gene Editing, Gene therapy and Immunotherapy Omics technologies Industrial Biotechnology, Biopharmaceuticals and Biocatalysis Bioprocess engineering and Downstream processing Plant Biotechnology Biosafety, Biotech Ethics, Science Communication Methods and Advances.