{"title":"富硒蛋清蛋白通过肠道菌群驱动的Htr1b抑制减轻糖皮质激素诱导的骨质疏松小鼠","authors":"Da Wang, Hongyu Zhang, Xiangheng Cao, Dongning Li, Ruiyang Qiu, Mengge Zhao","doi":"10.1111/1750-3841.70450","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Long-term glucocorticoid therapy is known to induce osteoporosis by disrupting bone metabolism and altering gut microbiota. In this study, C57BL/6J mice were used to establish a glucocorticoid-induced osteoporosis (GIOP) model by intramuscular injection of dexamethasone. The mice were then divided into control, GIOP, and experimental groups; the experimental group was orally administered selenium-enriched egg white protein (Se-EWP) in order to evaluate its bone-protective effects and potential mechanisms. The results showed that Se-EWP significantly improved bone strength, trabecular microstructure, and bone mineral density in GIOP mice; enhanced the expression of bone synthesis-related proteins, such as osterix and osteocalcin; and decreased the expression of bone catabolism-related proteins, such as tartrate-resistant acid phosphatase, receptor activator of nuclear factor-κB ligand, and cathepsin K. In addition, Se-EWP was found to alleviate glucocorticoid-induced gut microflora dysbiosis by increasing beneficial bacteria and microbial diversity. Se-EWP significantly modulated the 5-hydroxytryptamine receptor 1B (Htr1b) pathway, which is crucial for bone homeostasis and gut–bone communication. The modulation of the Htr1b pathway suggests that Se-EWP may exert its anti-osteoporotic effects through this mechanism. These effects were statistically significant (<i>p</i> < 0.05). Correlation analysis revealed significant associations between changes in the intestinal microbiota and bone metabolism parameters, further supporting the hypothesis that Se-EWP exerts its effects through the gut–bone axis. This suggests a novel therapeutic mechanism of action for Se-EWP through the gut–bone axis. In conclusion, this study provides strong evidence supporting the application of Se-EWP in the prevention and treatment of GIOP.</p>\n </div>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":"90 8","pages":""},"PeriodicalIF":3.4000,"publicationDate":"2025-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Selenium-Enriched Egg White Protein Alleviates Glucocorticoid-Induced Osteoporosis Mice via Gut Microbiota-Driven Htr1b Inhibition\",\"authors\":\"Da Wang, Hongyu Zhang, Xiangheng Cao, Dongning Li, Ruiyang Qiu, Mengge Zhao\",\"doi\":\"10.1111/1750-3841.70450\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>Long-term glucocorticoid therapy is known to induce osteoporosis by disrupting bone metabolism and altering gut microbiota. In this study, C57BL/6J mice were used to establish a glucocorticoid-induced osteoporosis (GIOP) model by intramuscular injection of dexamethasone. The mice were then divided into control, GIOP, and experimental groups; the experimental group was orally administered selenium-enriched egg white protein (Se-EWP) in order to evaluate its bone-protective effects and potential mechanisms. The results showed that Se-EWP significantly improved bone strength, trabecular microstructure, and bone mineral density in GIOP mice; enhanced the expression of bone synthesis-related proteins, such as osterix and osteocalcin; and decreased the expression of bone catabolism-related proteins, such as tartrate-resistant acid phosphatase, receptor activator of nuclear factor-κB ligand, and cathepsin K. In addition, Se-EWP was found to alleviate glucocorticoid-induced gut microflora dysbiosis by increasing beneficial bacteria and microbial diversity. Se-EWP significantly modulated the 5-hydroxytryptamine receptor 1B (Htr1b) pathway, which is crucial for bone homeostasis and gut–bone communication. The modulation of the Htr1b pathway suggests that Se-EWP may exert its anti-osteoporotic effects through this mechanism. These effects were statistically significant (<i>p</i> < 0.05). Correlation analysis revealed significant associations between changes in the intestinal microbiota and bone metabolism parameters, further supporting the hypothesis that Se-EWP exerts its effects through the gut–bone axis. This suggests a novel therapeutic mechanism of action for Se-EWP through the gut–bone axis. In conclusion, this study provides strong evidence supporting the application of Se-EWP in the prevention and treatment of GIOP.</p>\\n </div>\",\"PeriodicalId\":193,\"journal\":{\"name\":\"Journal of Food Science\",\"volume\":\"90 8\",\"pages\":\"\"},\"PeriodicalIF\":3.4000,\"publicationDate\":\"2025-08-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Food Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://ift.onlinelibrary.wiley.com/doi/10.1111/1750-3841.70450\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"FOOD SCIENCE & TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Food Science","FirstCategoryId":"97","ListUrlMain":"https://ift.onlinelibrary.wiley.com/doi/10.1111/1750-3841.70450","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
Selenium-Enriched Egg White Protein Alleviates Glucocorticoid-Induced Osteoporosis Mice via Gut Microbiota-Driven Htr1b Inhibition
Long-term glucocorticoid therapy is known to induce osteoporosis by disrupting bone metabolism and altering gut microbiota. In this study, C57BL/6J mice were used to establish a glucocorticoid-induced osteoporosis (GIOP) model by intramuscular injection of dexamethasone. The mice were then divided into control, GIOP, and experimental groups; the experimental group was orally administered selenium-enriched egg white protein (Se-EWP) in order to evaluate its bone-protective effects and potential mechanisms. The results showed that Se-EWP significantly improved bone strength, trabecular microstructure, and bone mineral density in GIOP mice; enhanced the expression of bone synthesis-related proteins, such as osterix and osteocalcin; and decreased the expression of bone catabolism-related proteins, such as tartrate-resistant acid phosphatase, receptor activator of nuclear factor-κB ligand, and cathepsin K. In addition, Se-EWP was found to alleviate glucocorticoid-induced gut microflora dysbiosis by increasing beneficial bacteria and microbial diversity. Se-EWP significantly modulated the 5-hydroxytryptamine receptor 1B (Htr1b) pathway, which is crucial for bone homeostasis and gut–bone communication. The modulation of the Htr1b pathway suggests that Se-EWP may exert its anti-osteoporotic effects through this mechanism. These effects were statistically significant (p < 0.05). Correlation analysis revealed significant associations between changes in the intestinal microbiota and bone metabolism parameters, further supporting the hypothesis that Se-EWP exerts its effects through the gut–bone axis. This suggests a novel therapeutic mechanism of action for Se-EWP through the gut–bone axis. In conclusion, this study provides strong evidence supporting the application of Se-EWP in the prevention and treatment of GIOP.
期刊介绍:
The goal of the Journal of Food Science is to offer scientists, researchers, and other food professionals the opportunity to share knowledge of scientific advancements in the myriad disciplines affecting their work, through a respected peer-reviewed publication. The Journal of Food Science serves as an international forum for vital research and developments in food science.
The range of topics covered in the journal include:
-Concise Reviews and Hypotheses in Food Science
-New Horizons in Food Research
-Integrated Food Science
-Food Chemistry
-Food Engineering, Materials Science, and Nanotechnology
-Food Microbiology and Safety
-Sensory and Consumer Sciences
-Health, Nutrition, and Food
-Toxicology and Chemical Food Safety
The Journal of Food Science publishes peer-reviewed articles that cover all aspects of food science, including safety and nutrition. Reviews should be 15 to 50 typewritten pages (including tables, figures, and references), should provide in-depth coverage of a narrowly defined topic, and should embody careful evaluation (weaknesses, strengths, explanation of discrepancies in results among similar studies) of all pertinent studies, so that insightful interpretations and conclusions can be presented. Hypothesis papers are especially appropriate in pioneering areas of research or important areas that are afflicted by scientific controversy.