双胞苷GD3对阳离子DODAB囊泡亚凝胶、凝胶和流体相的影响

IF 2.2 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biophysical chemistry Pub Date : 2025-08-04 DOI:10.1016/j.bpc.2025.107503

Julia B. Ejarque , Anna C.F. Couto , Thábata Matos , Evandro L. Duarte , M. Teresa Lamy , Julio H.K. Rozenfeld

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引用次数: 0

摘要

GD3是一种在几种类型的癌细胞中过表达的双胞脂苷。合成的阳离子脂质DODAB已成功用作疫苗佐剂，可增强GD3的免疫原性。本文采用差示扫描量热法（DSC）和电子顺磁共振（EPR）对GD3和DODAB的混合分散体进行了表征。GD3与DODAB存在混相，降低了DODAB凝胶-流体的迁移协同性。GD3不影响凝胶-流体和流体-凝胶转变之间的温度滞后。GD3不影响冷却至15℃以下的DODAB双分子层中亚凝胶相的形成。GD3减少了DODAB亚凝胶相的酰基链堆积，这可以解释混合分散体在5°C至20°C之间出现的宽而浅的放热事件。这些结果可能有助于开发新的基于gd3的癌症免疫疗法，包括在低温下参与冷链稳定性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Effect of disialoganglioside GD3 on the subgel, gel and fluid phases of cationic DODAB vesicles

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Effect of disialoganglioside GD3 on the subgel, gel and fluid phases of cationic DODAB vesicles

GD3 is a disialoganglioside overexpressed in several types of cancer cells. The synthetic cationic lipid DODAB has been successfully employed as a vaccine adjuvant, and would be suitable to enhance GD3 immunogenicity. Here, mixed dispersions of GD3 and DODAB were characterized by Differential Scanning Calorimetry (DSC) and Electron Paramagnetic Resonance (EPR) spectroscopy. GD3 is miscible with DODAB, and decreases the DODAB gel-fluid transition cooperativity. GD3 does not affect the temperature hysteresis between gel-fluid and fluid-gel transitions. GD3 does not affect the formation of a subgel phase in DODAB bilayers cooled below 15 °C. GD3 decreases the acyl chain packing of the DODAB subgel phase, which could explain the broad and shallow exothermic event between 5 °C and 20 °C that appears on thermograms of mixed dispersions. These results might contribute to the development of novel GD3-based cancer immunotherapies, including at the low temperatures involved in cold chain stability.

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来源期刊

Biophysical chemistry 生物-生化与分子生物学

CiteScore

6.10

自引率

10.50%

发文量

121

审稿时长

20 days

期刊介绍： Biophysical Chemistry publishes original work and reviews in the areas of chemistry and physics directly impacting biological phenomena. Quantitative analysis of the properties of biological macromolecules, biologically active molecules, macromolecular assemblies and cell components in terms of kinetics, thermodynamics, spatio-temporal organization, NMR and X-ray structural biology, as well as single-molecule detection represent a major focus of the journal. Theoretical and computational treatments of biomacromolecular systems, macromolecular interactions, regulatory control and systems biology are also of interest to the journal.