{"title":"脂肪来源的间充质干细胞外泌体和培养上清的蛋白质组学和代谢组学分析。","authors":"Toyofumi Hirakawa, Tamotsu Kato, Yumiko Nakanishi, Daichi Urushiyama, Kohei Miyata, Tsukasa Baba, Hiroshi Ohno, Shin'ichiro Yasunaga, Fusanori Yotsumoto, Shingo Miyamoto","doi":"10.21873/anticanres.17703","DOIUrl":null,"url":null,"abstract":"<p><strong>Background/aim: </strong>Aberrant differentiation of fertilized eggs during <i>in vitro</i> fertilization is a major contributor to infertility, and adipose-derived mesenchymal stem cells (ASCs) and their exosomes have been reported to facilitate fertilized egg differentiation; however, the underlying mechanisms remain unclear. This study aimed to elucidate how ASC-derived exosomes promote fertilized egg differentiation through proteomic analysis of ASC-derived exosomal proteins and metabolomic profiling of culture supernatants.</p><p><strong>Materials and methods: </strong>Extracellular vesicles were isolated from ASC culture supernatants <i>via</i> differential ultracentrifugation, proteins were extracted and digested using a phase-transfer surfactant protocol for LC-MS/MS analysis, and metabolites were analyzed using GC-MS/MS following extraction and derivatization.</p><p><strong>Results: </strong>Exosomes from early passage ASCs were enriched with various proteins, including alpha and beta proteasome subunits, which exhibit proteasome activity. Gene ontology analysis revealed the presence of proteins associated with transmembrane transport and cytoplasmic translation. Metabolomic profiling of culture supernatants revealed markedly elevated levels of amino acids associated with glycine, serine, and methionine metabolism.</p><p><strong>Conclusion: </strong>ASC-derived culture supernatants can exert differentiation-promoting effects on fertilized eggs <i>via</i> exosomal proteins and soluble metabolites and may therefore be a novel option for infertility treatment.</p>","PeriodicalId":8072,"journal":{"name":"Anticancer research","volume":"45 8","pages":"3409-3424"},"PeriodicalIF":1.7000,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Proteomic and Metabolomic Analyses of Adipose-derived Mesenchymal Stem Cell Exosomes and Culture Supernatants.\",\"authors\":\"Toyofumi Hirakawa, Tamotsu Kato, Yumiko Nakanishi, Daichi Urushiyama, Kohei Miyata, Tsukasa Baba, Hiroshi Ohno, Shin'ichiro Yasunaga, Fusanori Yotsumoto, Shingo Miyamoto\",\"doi\":\"10.21873/anticanres.17703\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background/aim: </strong>Aberrant differentiation of fertilized eggs during <i>in vitro</i> fertilization is a major contributor to infertility, and adipose-derived mesenchymal stem cells (ASCs) and their exosomes have been reported to facilitate fertilized egg differentiation; however, the underlying mechanisms remain unclear. This study aimed to elucidate how ASC-derived exosomes promote fertilized egg differentiation through proteomic analysis of ASC-derived exosomal proteins and metabolomic profiling of culture supernatants.</p><p><strong>Materials and methods: </strong>Extracellular vesicles were isolated from ASC culture supernatants <i>via</i> differential ultracentrifugation, proteins were extracted and digested using a phase-transfer surfactant protocol for LC-MS/MS analysis, and metabolites were analyzed using GC-MS/MS following extraction and derivatization.</p><p><strong>Results: </strong>Exosomes from early passage ASCs were enriched with various proteins, including alpha and beta proteasome subunits, which exhibit proteasome activity. Gene ontology analysis revealed the presence of proteins associated with transmembrane transport and cytoplasmic translation. Metabolomic profiling of culture supernatants revealed markedly elevated levels of amino acids associated with glycine, serine, and methionine metabolism.</p><p><strong>Conclusion: </strong>ASC-derived culture supernatants can exert differentiation-promoting effects on fertilized eggs <i>via</i> exosomal proteins and soluble metabolites and may therefore be a novel option for infertility treatment.</p>\",\"PeriodicalId\":8072,\"journal\":{\"name\":\"Anticancer research\",\"volume\":\"45 8\",\"pages\":\"3409-3424\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2025-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Anticancer research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.21873/anticanres.17703\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"ONCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Anticancer research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.21873/anticanres.17703","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ONCOLOGY","Score":null,"Total":0}
Proteomic and Metabolomic Analyses of Adipose-derived Mesenchymal Stem Cell Exosomes and Culture Supernatants.
Background/aim: Aberrant differentiation of fertilized eggs during in vitro fertilization is a major contributor to infertility, and adipose-derived mesenchymal stem cells (ASCs) and their exosomes have been reported to facilitate fertilized egg differentiation; however, the underlying mechanisms remain unclear. This study aimed to elucidate how ASC-derived exosomes promote fertilized egg differentiation through proteomic analysis of ASC-derived exosomal proteins and metabolomic profiling of culture supernatants.
Materials and methods: Extracellular vesicles were isolated from ASC culture supernatants via differential ultracentrifugation, proteins were extracted and digested using a phase-transfer surfactant protocol for LC-MS/MS analysis, and metabolites were analyzed using GC-MS/MS following extraction and derivatization.
Results: Exosomes from early passage ASCs were enriched with various proteins, including alpha and beta proteasome subunits, which exhibit proteasome activity. Gene ontology analysis revealed the presence of proteins associated with transmembrane transport and cytoplasmic translation. Metabolomic profiling of culture supernatants revealed markedly elevated levels of amino acids associated with glycine, serine, and methionine metabolism.
Conclusion: ASC-derived culture supernatants can exert differentiation-promoting effects on fertilized eggs via exosomal proteins and soluble metabolites and may therefore be a novel option for infertility treatment.
期刊介绍：
ANTICANCER RESEARCH is an independent international peer-reviewed journal devoted to the rapid publication of high quality original articles and reviews on all aspects of experimental and clinical oncology. Prompt evaluation of all submitted articles in confidence and rapid publication within 1-2 months of acceptance are guaranteed.
ANTICANCER RESEARCH was established in 1981 and is published monthly (bimonthly until the end of 2008). Each annual volume contains twelve issues and index. Each issue may be divided into three parts (A: Reviews, B: Experimental studies, and C: Clinical and Epidemiological studies).
Special issues, presenting the proceedings of meetings or groups of papers on topics of significant progress, will also be included in each volume. There is no limitation to the number of pages per issue.
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