基于体外方法的转录组学和代谢组学摄取标准在调节毒理学中的重要作用。

IF 6.9 2区 医学 Q1 TOXICOLOGY
Julia M. Malinowska, Maurice Whelan
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引用次数: 0

摘要

由于越来越多的监管机构对应用基于组学的方法和减少动物实验感兴趣,本研究回顾了现有的转录组学和代谢组学文献标准,重点关注那些适用于体外测试系统的标准。调查显示,情况是不一样的:对于转录组学(使用RNA-seq),一些文献标准已经由正式的标准化机构(如国际标准化组织)制定,而对于代谢组学(使用质谱),这些主要是由科学界开发最佳实践的工作推动的。标准物质的价值也被强调,因为它们能够表征(分析)实验室内的可重复性和可重复性以及实验室间的可重复性。利用进入经合组织测试指南计划的基于组学的体外方法中的标准,将确保其可靠性、跨司法管辖区的可及性和长期使用的可持续性。通过实际例子解释了这些好处,并说明了如何更好地利用现有标准和启动有针对性的新标准,以便根据体外转录组学和代谢组学方法高效和有效地制定国际测试指南。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The important role of standards for the uptake of transcriptomics and metabolomics based in vitro methods in regulatory toxicology

Driven by increasing regulatory interest in applying omics-based methods and reducing animal testing, this study reviewed existing documentary standards for transcriptomics and metabolomics, focusing on those applicable to in vitro test systems. Investigation revealed the landscape to be heterogeneous: for transcriptomics (using RNA-seq), some documentary standards have been produced by formal standardisation bodies (e.g., International Organization for Standardization), whilst for metabolomics (using MS), these were primarily driven by the work of the scientific community developing best practices. The value of reference materials is also highlighted since they enable characterisation of both (analytical) intra-laboratory repeatability and reproducibility as well as inter-laboratory reproducibility. Leveraging standards within omics-based in vitro methods that enter the OECD Test Guideline Programme will ensure their reliability, accessibility across jurisdictions, and sustainability for their long-term use. These benefits are explained with practical examples and make the case for better use of existing standards and initiating targeted development of new standards for efficient and effective development of international Test Guidelines based on in vitro transcriptomics and metabolomics methods.

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来源期刊
Archives of Toxicology
Archives of Toxicology 医学-毒理学
CiteScore
11.60
自引率
4.90%
发文量
218
审稿时长
1.5 months
期刊介绍: Archives of Toxicology provides up-to-date information on the latest advances in toxicology. The journal places particular emphasis on studies relating to defined effects of chemicals and mechanisms of toxicity, including toxic activities at the molecular level, in humans and experimental animals. Coverage includes new insights into analysis and toxicokinetics and into forensic toxicology. Review articles of general interest to toxicologists are an additional important feature of the journal.
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