Laurie Josset , Mathilde Mura , Mathis Damon , Emma de Cartier d’Yves , Michèle Weiss-Gayet , Anaelle Boreau , Emeraude Rivoire , Nellie Della-Schiava , Anne Long , Sandrine Horman , Antoine Millon , Vincent Pialoux , Amandine Thomas
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Regular physical activity (PA) has been shown to modulate hemostasis by reducing fibrinogen concentration and platelet activation, though its effects on coagulation are limited.</div></div><div><h3>Objectives</h3><div>We aim to evaluate the procoagulant and proinflammatory state in asymptomatic patients with atherosclerotic carotid stenosis, compared to healthy subjects and to assess the impact of a 6-month PA intervention.</div></div><div><h3>Methods</h3><div>This study included patients with asymptomatic carotid stenosis and healthy subjects. Patients were randomized into a PA group (6-month individualized PA intervention), and a control group. Blood analyses were performed to assess monocyte phenotype and TF expression by flow cytometry, and coagulation parameters using rotative thromboelastometry.</div></div><div><h3>Results</h3><div>The expression of TF on the surface of circulating monocytes was significantly higher in patients than in healthy subjects (61.9 ± 11.7 % vs 33.7 ± 6.3 %, p < 0.0001), mainly explained by a higher expression on classical monocytes (77.7 ± 9.5 vs 58.3 ± 15.8 %, p < 0.001). Clotting times were lower in patients than in healthy subjects in basal (407.8 77.7 ± 144.6 s vs 615.5 ± 123.4 s p < 0.0001) and proinflammatory (222.4 ± 48.2 s vs 307.8 ± 82.4 s p = 0.003) conditions. A 6-month PA intervention did not induce any modulation of the monocyte phenotype or coagulation parameters of patients with atherosclerosis.</div></div><div><h3>Conclusion</h3><div>Asymptomatic patients with carotid atherosclerosis have a higher proinflammatory and procoagulant profile, suggesting a higher thromboembolic risk. However, home-based PA intervention did not improve their profiles.</div></div>","PeriodicalId":34401,"journal":{"name":"Thrombosis Update","volume":"20 ","pages":"Article 100219"},"PeriodicalIF":0.0000,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Asymptomatic patients with carotid atherosclerosis present a higher coagulation state compared to healthy subjects\",\"authors\":\"Laurie Josset , Mathilde Mura , Mathis Damon , Emma de Cartier d’Yves , Michèle Weiss-Gayet , Anaelle Boreau , Emeraude Rivoire , Nellie Della-Schiava , Anne Long , Sandrine Horman , Antoine Millon , Vincent Pialoux , Amandine Thomas\",\"doi\":\"10.1016/j.tru.2025.100219\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div>In pro-inflammatory conditions, such as atherosclerosis, circulating monocytes may express tissue factor (TF), increasing thromboembolic risk. Regular physical activity (PA) has been shown to modulate hemostasis by reducing fibrinogen concentration and platelet activation, though its effects on coagulation are limited.</div></div><div><h3>Objectives</h3><div>We aim to evaluate the procoagulant and proinflammatory state in asymptomatic patients with atherosclerotic carotid stenosis, compared to healthy subjects and to assess the impact of a 6-month PA intervention.</div></div><div><h3>Methods</h3><div>This study included patients with asymptomatic carotid stenosis and healthy subjects. Patients were randomized into a PA group (6-month individualized PA intervention), and a control group. Blood analyses were performed to assess monocyte phenotype and TF expression by flow cytometry, and coagulation parameters using rotative thromboelastometry.</div></div><div><h3>Results</h3><div>The expression of TF on the surface of circulating monocytes was significantly higher in patients than in healthy subjects (61.9 ± 11.7 % vs 33.7 ± 6.3 %, p < 0.0001), mainly explained by a higher expression on classical monocytes (77.7 ± 9.5 vs 58.3 ± 15.8 %, p < 0.001). Clotting times were lower in patients than in healthy subjects in basal (407.8 77.7 ± 144.6 s vs 615.5 ± 123.4 s p < 0.0001) and proinflammatory (222.4 ± 48.2 s vs 307.8 ± 82.4 s p = 0.003) conditions. A 6-month PA intervention did not induce any modulation of the monocyte phenotype or coagulation parameters of patients with atherosclerosis.</div></div><div><h3>Conclusion</h3><div>Asymptomatic patients with carotid atherosclerosis have a higher proinflammatory and procoagulant profile, suggesting a higher thromboembolic risk. 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引用次数: 0
摘要
背景:在促炎条件下,如动脉粥样硬化,循环单核细胞可能表达组织因子(TF),增加血栓栓塞的风险。有规律的体育活动(PA)已被证明通过降低纤维蛋白原浓度和血小板活化来调节止血,尽管其对凝血的影响有限。目的:我们的目的是评估无症状的动脉粥样硬化性颈动脉狭窄患者的促凝和促炎状态,与健康受试者进行比较,并评估6个月PA干预的影响。方法本研究包括无症状颈动脉狭窄患者和健康受试者。患者被随机分为PA组(6个月个体化PA干预)和对照组。进行血液分析,通过流式细胞术评估单核细胞表型和TF表达,并使用旋转血栓弹性仪评估凝血参数。结果患者外周血单核细胞表面TF表达明显高于正常对照组(61.9±11.7% vs 33.7±6.3%,p <;0.0001),主要原因是在经典单核细胞中表达较高(77.7±9.5% vs 58.3±15.8%,p <;0.001)。患者凝血时间低于健康对照组(407.8±77.7±144.6 s vs 615.5±123.4 s p <;0.0001)和促炎(222.4±48.2 s vs 307.8±82.4 s p = 0.003)。6个月的PA干预没有引起动脉粥样硬化患者单核细胞表型或凝血参数的任何调节。结论无症状颈动脉粥样硬化患者具有较高的促炎和促凝特征,提示血栓栓塞风险较高。然而,以家庭为基础的PA干预并没有改善他们的档案。
Asymptomatic patients with carotid atherosclerosis present a higher coagulation state compared to healthy subjects
Background
In pro-inflammatory conditions, such as atherosclerosis, circulating monocytes may express tissue factor (TF), increasing thromboembolic risk. Regular physical activity (PA) has been shown to modulate hemostasis by reducing fibrinogen concentration and platelet activation, though its effects on coagulation are limited.
Objectives
We aim to evaluate the procoagulant and proinflammatory state in asymptomatic patients with atherosclerotic carotid stenosis, compared to healthy subjects and to assess the impact of a 6-month PA intervention.
Methods
This study included patients with asymptomatic carotid stenosis and healthy subjects. Patients were randomized into a PA group (6-month individualized PA intervention), and a control group. Blood analyses were performed to assess monocyte phenotype and TF expression by flow cytometry, and coagulation parameters using rotative thromboelastometry.
Results
The expression of TF on the surface of circulating monocytes was significantly higher in patients than in healthy subjects (61.9 ± 11.7 % vs 33.7 ± 6.3 %, p < 0.0001), mainly explained by a higher expression on classical monocytes (77.7 ± 9.5 vs 58.3 ± 15.8 %, p < 0.001). Clotting times were lower in patients than in healthy subjects in basal (407.8 77.7 ± 144.6 s vs 615.5 ± 123.4 s p < 0.0001) and proinflammatory (222.4 ± 48.2 s vs 307.8 ± 82.4 s p = 0.003) conditions. A 6-month PA intervention did not induce any modulation of the monocyte phenotype or coagulation parameters of patients with atherosclerosis.
Conclusion
Asymptomatic patients with carotid atherosclerosis have a higher proinflammatory and procoagulant profile, suggesting a higher thromboembolic risk. However, home-based PA intervention did not improve their profiles.