人诱导多能干细胞衍生肺类器官用于呼吸道感染研究。

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2025-07-31 DOI:10.1007/7651_2025_660

Sandra L Leibel

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引用次数: 0

摘要

人类诱导多能干细胞（hiPSC）衍生的肺类器官的发展已经彻底改变了肺发育、疾病建模和感染系统的研究。这些三维结构概括了人类肺生理学的关键特征，包括上皮和间充质相互作用，并为研究病毒感染、上皮特异性免疫反应和治疗干预提供了生物学相关的平台。在这里，我们概述了从hipsc逐步衍生的肺类器官，描述了它们的特性，并讨论了它们在感染建模中的应用。

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Derivation of Lung Organoids from Human-Induced Pluripotent Stem Cells for Respiratory Infection Studies.

The development of human-induced pluripotent stem cell (hiPSC)-derived lung organoids has revolutionized the study of lung development, disease modeling, and infection systems. These three-dimensional structures recapitulate key features of human lung physiology, including epithelial and mesenchymal interactions, and provide a biologically relevant platform for studying viral infections, epithelial-specific immune responses, and therapeutic interventions. Here, we outline the stepwise derivation of lung organoids from hiPSCs, describe their characterization, and discuss their application in infection modeling.

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.