人血小板中udp -葡萄糖醛基转移酶mRNA的鉴定：吸烟者和非吸烟者的差异水平。

IF 2.6 3区医学 Q3 CELL BIOLOGY

Platelets Pub Date : 2025-12-01 Epub Date: 2025-07-28 DOI:10.1080/09537104.2025.2536306

Yazun Jarrar, Mansour Almansour, Dina Abulebdeh, Wesam Nasser, Qais Jarrar, Sara Abudahab, Su-Jun Lee

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引用次数: 0

摘要

背景：udp -葡萄糖醛酸转移酶（UGTs）是II期药物代谢的主要酶，但其在人血小板中的表达仍未得到充分研究。目的：本研究旨在表征人类血小板中UGT亚型的mRNA表达谱，并评估吸烟者和非吸烟者之间的差异。方法：从富血小板血浆（PRP）、黄皮毛（BC）和肝脏样品中提取总RNA。逆转录后，采用PCR检测UGT亚型UGT1A1、1A3、1A4、1A5、1A6、1A7、1A8、1A9、1A10、2B7和2B15。量化PRP中UGT1A5和2B15的mRNA水平，并与肝脏和BC中的水平进行比较。吸烟者和非吸烟者的PRP样本也进行了分析。结果：人血小板中检测到UGT1A5和2B15 mrna。UGT1A5在PRP中的表达明显高于肝脏，而在肝脏中的表达则低约三分之一。与不吸烟者相比，吸烟者的血小板中UGT1A5和UGT2B15基因的表达分别增加了11.3倍和3.3倍。结论：本研究结论UGT1A5和2B15 mRNA在人类血小板中存在，且在吸烟者血小板中表达水平升高。需要进一步的研究，包括药代动力学研究和蛋白水平验证，以了解它们的功能意义。

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Identification of UDP-Glucuronosyltransferase mRNA in human platelets: differential levels in smokers and non-smokers.

Background: UDP-glucuronosyl transferases (UGTs) are major enzymes of phase II drug metabolism, yet their expression in human platelets remains underexplored. Objectives: This study aimed to characterize the mRNA expression profile of UGT isoforms in human platelets and to assess differences between cigarette smokers and nonsmokers.

Methods: Total RNA was extracted from platelet-rich plasma (PRP), buffy coat (BC), and liver samples. After reverse transcription, PCR was performed to detect UGT isoforms including UGT1A1, 1A3, 1A4, 1A5, 1A6, 1A7, 1A8, 1A9, 1A10, 2B7, and 2B15. The mRNA levels of UGT1A5 and 2B15 in PRP were quantified and compared to levels in liver and BC. PRP samples from smokers and nonsmokers were also analyzed.

Results: UGT1A5 and 2B15 mRNAs were detected in human platelets. UGT1A5 expression was significantly higher in PRP than in liver, while it was about threefold lower. Both genes showed elevated expression in smokers' platelets, approximately 11.3-fold for UGT1A5 and 3.3-fold for UGT2B15, compared to nonsmokers.

Conclusions: This study concludes the presence of UGT1A5 and 2B15 mRNA in human platelets and their elevated level in the platelets of smokers. Further research, including pharmacokinetic studies and protein-level validation, is needed to understand their functional significance.

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来源期刊

Platelets 医学-细胞生物学

CiteScore

6.70

自引率

3.00%

发文量

审稿时长

1 months

期刊介绍： Platelets is an international, peer-reviewed journal covering all aspects of platelet- and megakaryocyte-related research. Platelets provides the opportunity for contributors and readers across scientific disciplines to engage with new information about blood platelets. The journal’s Methods section aims to improve standardization between laboratories and to help researchers replicate difficult methods. Research areas include: Platelet function Biochemistry Signal transduction Pharmacology and therapeutics Interaction with other cells in the blood vessel wall The contribution of platelets and platelet-derived products to health and disease The journal publishes original articles, fast-track articles, review articles, systematic reviews, methods papers, short communications, case reports, opinion articles, commentaries, gene of the issue, and letters to the editor. Platelets operates a single-blind peer review policy. Authors can choose to publish gold open access in this journal.