母体接触三丁基锡会改变女性生殖系统的发育。

IF 3.9 3区 医学 Q2 ENDOCRINOLOGY & METABOLISM
Journal of Endocrinology Pub Date : 2025-08-11 Print Date: 2025-08-01 DOI:10.1530/JOE-24-0357
Rosiane Aparecida Miranda, Beatriz Souza da Silva, Iala Milene Bertasso, Luana Lopes de Souza, Edgar Mendes Souza Wan Der Maas, Jones Bernardes Graceli, Leandro Miranda-Alves, Egberto Gaspar de Moura, Patricia Cristina Lisboa
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引用次数: 0

摘要

三丁基锡(TBT)是一种用于防污涂料的有毒化合物,以其内分泌干扰特性而闻名,包括女性生殖功能障碍。我们假设在妊娠期和哺乳期接触TBT会导致雌性后代的长期生殖改变。妊娠Wistar大鼠从妊娠第7天至哺乳期结束,分别口服0.01%乙醇(对照组)、TBT 100 ng/kg或TBT 1000 ng/kg体重。雌性后代在产后21、45和180天(PND)评估生物特征、激素和卵巢参数。TBT100ng通过PND180降低出生体重,TBT1000ng通过PND180降低体重。在PND45时,TBT组和TBT组的睾酮水平均升高,而TBT组的FSH水平则下降。在TBT1000ng组中可观察到性周期异常,如延长的流星-迟发期。卵巢分析显示PND21和PND45时囊性和闭锁性卵泡数量增加。在PND180中观察到原始卵泡(TBT100ng)和黄体(两个TBT组)数量减少,并伴有卵巢纤维化。TBT暴露导致卵巢卵泡动力学的年龄和剂量依赖性破坏,PND21时健康卵泡最初增加,随后不健康卵泡升高,后期健康卵泡减少。在PND21时,两种TBT剂量均增加ERα表达,而TBT100ng增加AR表达。这些变化伴随着卵巢肥大细胞数量的持续增加和IL-6蛋白表达的升高,特别是在PND21和PND180。因此,母体接触TBT会破坏卵巢发育和功能,潜在地增加多囊卵巢综合征和原发性卵巢功能不全等异常情况的易感性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Maternal exposure to tributyltin alters female reproductive system development.

Graphical abstract:

Abstract: Tributyltin (TBT) is a toxic compound used in antifouling paints and known for its endocrine-disrupting properties, including female reproductive dysfunction. We hypothesized that TBT exposure during gestation and lactation induces long-term reproductive alterations in female offspring. Pregnant Wistar rats were orally exposed from gestational day 7 to the end of lactation to 0.01% ethanol (control), TBT 100 ng/kg, or TBT 1000 ng/kg body weight. Female offspring were evaluated at postnatal days (PND) 21, 45, and 180 for biometric, hormonal, and ovarian parameters. Birth weight was reduced in the TBT100ng group, and body weight was reduced by PND180 in the TBT1000ng group. At PND45, testosterone increased in both TBT groups, while FSH decreased in the TBT100ng group. Estrous cyclicity irregularities, such as a prolonged metestrus-diestrus phase, were noted in the TBT1000ng group. Ovarian analysis showed increased cystic and atretic follicles at PND21 and PND45. Reduction in primordial follicles (TBT100ng) and corpora lutea (both TBT groups) was observed at PND180, along with ovarian fibrosis. TBT exposure led to age- and dose-dependent disruptions in ovarian follicle dynamics: initial increases in healthy follicles at PND21, followed by elevated unhealthy follicles and reduced healthy ones at later stages. At PND21, both TBT doses increased ERα expression, while TBT100ng increased AR expression. These changes were accompanied by a persistent increase in ovarian mast cells and elevated IL-6 protein expression, particularly at PND21 and PND180. Thus, maternal TBT exposure disrupts ovarian development and function, potentially increasing susceptibility to abnormal conditions such as polycystic ovary syndrome and primary ovarian insufficiency later in life.

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来源期刊
Journal of Endocrinology
Journal of Endocrinology 医学-内分泌学与代谢
CiteScore
7.90
自引率
2.50%
发文量
113
审稿时长
4-8 weeks
期刊介绍: Journal of Endocrinology is a leading global journal that publishes original research articles, reviews and science guidelines. Its focus is on endocrine physiology and metabolism, including hormone secretion; hormone action; biological effects. The journal publishes basic and translational studies at the organ, tissue and whole organism level.
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