褐藻中新的细胞毒性一元藻烷和一元藻烷二萜

Kolukula Ashwini , Bandi Siva , Penta Poornima , Solipeta Divya Reddy , Hashnu Dutta , Vedula Girija Sastry , Katragadda Suresh Babu
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引用次数: 0

摘要

从泰尔纳德邦Mandapam海岸采集的褐藻Dictyota dichotoma有机提取物中分离出5个新的dolabellane(1-5)、3个dolabellane(6-8)型二萜和5个先前鉴定的同源物(9-13)。新分离物1 ~ 8的结构和相对立体化学是通过广泛的波谱(NMR和质谱)数据确定的,10和12的结构是通过x射线衍射分析验证的。还提出了未描述的化合物1-8之间似是而非的生物遗传学关系。采用MTT法检测分离物对一系列癌细胞系的体外抗癌活性,包括DU145(前列腺)、B16F10(黑色素瘤)、HeLa(宫颈癌)和MDA-MB231(乳腺癌)。筛选结果表明,大多数分离的化合物对所测试的细胞系具有中等到强效的活性。其中化合物4和7对B16F10和DU145细胞的IC50值分别为3.53±0.05和2.18±0.06 μM。荧光、划痕实验和流式细胞术分析显示,化合物4和7抑制细胞增殖,阻滞G0期和G0/G1期细胞周期,诱导细胞凋亡死亡。综上所述,化合物4和7可以作为先导分子开发有效的抗癌药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
New cytotoxic dolabellane and dolastane diterpenes from Brown seaweed Dictyota dichotoma
Five new dolabellane (15), three dolastane (68) type diterpenes together with five previously identified congeners (913), were isolated from the organic extracts of the brown seaweed Dictyota dichotoma, collected in the Mandapam coast, Tamil Nadu. The structures and relative stereochemistry of the new isolates 18 were determined on the basis of extensive spectroscopic (NMR and Mass spec) data, whereas the structures of 10 and 12 were verified by X-ray diffraction analysis. A plausible biogenetic relationship between undescribed compounds 18 were also proposed. The in vitro anti-cancer activity of the isolates was examined against a panel of cancer cell lines, including DU145 (prostate), B16F10 (melanoma), HeLa (cervical), and MDA-MB231 (breast) using MTT assay. The screening results showed that majority of the isolated compounds exhibited moderate to potent activities against tested cell lines. Among the tested, compounds 4 and 7 manifested potent activities with an IC50 value of 3.53 ± 0.05 and 2.18 ± 0.06 μM respectively, against B16F10 and DU145 cells. Further, detailed fluorescence assays, scratch assay and flow cytometry analysis revealed that the compounds 4 and 7 diminished proliferation and arrested cell cycle in the G0 phase and G0/G1 phase, which induced cell death by apoptosis. Overall, this study provided that compounds 4 and 7 could serve as lead molecules for the development of potent anti-cancer agents.
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