进行性多发性硬化症小鼠的组织常驻记忆CD4 + T细胞浸润中枢神经系统并参与慢性自身免疫

IF 14.6 1区 医学 Q1 CELL BIOLOGY
Aurora Pignata, David Frieser, Carmen Gonzalez-Fierro, Cheng-Chih Hsiao, Hendrik J. Engelenburg, Marine Alis, Ilan Fijalkow, Vincent Cazaentre, Lucie Nozeran, Romain Miranda-Capet, Eloïse Dufourd, Thaïs Vermeulen, Amel Aïda, Carole Le Coz, Klaas Van Gisbergen, Nicolas Blanchard, Jörg Hamann, Joost Smolders, Roland S. Liblau, Frederick Masson
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引用次数: 0

摘要

防止T细胞迁移到中枢神经系统(CNS)对复发-缓解型多发性硬化症(RRMS)有显著的治疗效果,但对进行性多发性硬化症(PMS)效果不佳。经前综合症的残疾进展可能是由闷烧的局部炎症和神经变性之间的相互作用造成的。维持经前综合症慢性的机制尚不清楚。在这里,我们研究了组织驻留记忆CD4 + T细胞(CD4 + Trm细胞)在维持慢性中枢神经系统自身免疫中的潜在作用。我们发现CD4 + Trm细胞存在于慢性实验性自身免疫性脑脊髓炎(EAE)小鼠的中枢神经系统和经前综合征患者的脑组织中。通过流式细胞术和免疫组织荧光分析,我们发现在EAE小鼠的中枢神经系统和PMS患者的大脑中存在真正的CD4 + Trm细胞,表达特征性的Trm细胞表面标志物,包括CD69、CXCR6、P2RX7和CD49a。这些T细胞在慢性EAE小鼠中也表达转录因子Hobit。单细胞转录组学分析揭示了CD4 + Trm细胞的转录异质性和炎症潜能,因此,这些细胞定位于EAE小鼠和PMS患者的中枢神经系统炎症病变。最后,CD4 + Trm细胞的遗传或药物耗竭结合抗体介导的再循环CD4 + T细胞室耗竭缓解了EAE慢性期的神经症状。我们的研究结果表明,CD4 + Trm细胞有助于维持中枢神经系统的慢性炎症状态,并建议PMS的治疗策略应考虑靶向中枢神经系统驻留的T细胞室。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Tissue-resident memory CD4+ T cells infiltrate the CNS in progressive multiple sclerosis and contribute to chronic autoimmunity in mice
Preventing T cell migration to the central nervous system (CNS) has remarkable therapeutic effects in relapsing-remitting multiple sclerosis (RRMS) but is poorly effective against the progressive form (PMS). Disability progression in PMS likely results from an interplay between smoldering local inflammation and neurodegeneration. The mechanisms sustaining the chronicity of PMS are poorly understood. Here, we investigated the potential role of tissue-resident memory CD4+ T cells (CD4+ Trm cells) in sustaining chronic CNS autoimmunity. We showed that CD4+ Trm cells were present in the CNS of mice with chronic experimental autoimmune encephalomyelitis (EAE) and in brain tissues from persons with PMS. Using flow cytometry and immunohistofluorescence analysis, we revealed the presence of bona fide CD4+ Trm cells expressing characteristic Trm cell surface markers, including CD69, CXCR6, P2RX7, and CD49a, in the CNS of mice with EAE and in the brains of persons with PMS. These T cells also expressed the transcription factor Hobit in mice with chronic EAE. Single-cell transcriptomic analysis uncovered the transcriptional heterogeneity and inflammatory potential of CD4+ Trm cells, and, accordingly, these cells localized within CNS inflammatory lesions of mice with EAE and persons with PMS. Last, either genetic or pharmacological depletion of CD4+ Trm cells combined with antibody-mediated depletion of the recirculating CD4+ T cell compartment alleviated neurological signs during the chronic phase of EAE. Our results indicate that CD4+ Trm cells contribute to maintain a chronic inflammatory state in the CNS and suggest that therapeutic strategies for PMS should consider targeting the CNS-resident T cell compartment.
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来源期刊
Science Translational Medicine
Science Translational Medicine CELL BIOLOGY-MEDICINE, RESEARCH & EXPERIMENTAL
CiteScore
26.70
自引率
1.20%
发文量
309
审稿时长
1.7 months
期刊介绍: Science Translational Medicine is an online journal that focuses on publishing research at the intersection of science, engineering, and medicine. The goal of the journal is to promote human health by providing a platform for researchers from various disciplines to communicate their latest advancements in biomedical, translational, and clinical research. The journal aims to address the slow translation of scientific knowledge into effective treatments and health measures. It publishes articles that fill the knowledge gaps between preclinical research and medical applications, with a focus on accelerating the translation of knowledge into new ways of preventing, diagnosing, and treating human diseases. The scope of Science Translational Medicine includes various areas such as cardiovascular disease, immunology/vaccines, metabolism/diabetes/obesity, neuroscience/neurology/psychiatry, cancer, infectious diseases, policy, behavior, bioengineering, chemical genomics/drug discovery, imaging, applied physical sciences, medical nanotechnology, drug delivery, biomarkers, gene therapy/regenerative medicine, toxicology and pharmacokinetics, data mining, cell culture, animal and human studies, medical informatics, and other interdisciplinary approaches to medicine. The target audience of the journal includes researchers and management in academia, government, and the biotechnology and pharmaceutical industries. It is also relevant to physician scientists, regulators, policy makers, investors, business developers, and funding agencies.
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