高压氧治疗对老年患者成骨细胞钙化及骨保护素的影响

IF 1.5 Q3 ORTHOPEDICS

Journal of orthopaedics Pub Date : 2025-07-03 DOI:10.1016/j.jor.2025.06.017

Lisa Oezel , Johannes Schneppendahl , Jan-Peter Grassmann , Pia Flender , Sven Dreyer , Vera Grotheer

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引用次数: 0

摘要

背景骨质疏松症是世界上最常见的骨骼疾病之一。本研究旨在探讨高压氧（HBO）治疗和降低活性氧（ROS）的物质（如抗氧化剂）对老年髋关节置换术患者成骨细胞成骨分化和骨质疏松相关关键参数的影响。方法采用平均年龄73岁的22例患者（女性78.3%）的股骨头成骨细胞进行体外研究。通过双能x线骨密度仪（DXA）评估骨密度（BMD），将患者分为正常（与年龄相适应）骨密度（n = 8）、骨质减少（n = 6）和骨质疏松（n = 8）三组。诱导成骨分化，并给予HBO治疗21天。此外，用过氧化氢酶处理成骨细胞。评估与成骨分化和骨质疏松相关的参数。结果shbo治疗21 d后，3个实验组均促进成骨分化，成骨细胞骨密度与年龄相适应的差异有统计学意义（p = 0.0125）。此外，碱性磷酸酶（一种指示骨合成的酶）的活性在HBO治疗后的所有组中均显著增加(正常骨密度和骨质疏松细胞：p = 0.04；骨减少细胞：p = 0.006)。结论高压氧治疗可作为一种辅助治疗或研究性治疗，用于老年患者增强骨密度或促进骨折后骨愈合，特别是对于骨密度正常或骨质减少的患者。这种方法可能会影响未来的临床实践。

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Effect of hyperbaric oxygen therapy (HBO) on osteoblasts of elderly patients on calcification and osteoprotegerin

Background

Osteoporosis stands as one of the most prevalent bone diseases worldwide. This study aims to explore the effects of Hyperbaric Oxygen (HBO) therapy and substances that reduce reactive oxygen species (ROS), such as antioxidants, on osteogenic differentiation and key osteoporosis-related parameters in osteoblasts derived from elderly patients requiring hip arthroplasty.

Methods

An in vitro study was conducted using osteoblasts isolated from the femoral heads of 22 patients (78.3 % female) with a mean age of 73 years. Bone mineral density (BMD) was assessed through dual-energy X-ray absorptiometry (DXA), classifying patients into three groups: normal (age-appropriate) BMD (n = 8), osteopenia (n = 6), and osteoporosis (n = 8). Osteogenic differentiation was induced, and HBO therapy was administered over a period of 21 days. Additionally, osteoblasts were treated with catalase. Parameters related to osteogenic differentiation and osteoporosis were evaluated.

Results

HBO therapy prompted osteogenic differentiation in all three experimental groups after 21 days, with statistically significant findings (p = 0.0125) in osteoblasts with age-appropriate bone density. Furthermore, the activity of alkaline phosphatase (ALP), an enzyme indicative of bone synthesis, demonstrated significant increases across all groups following HBO treatment (normal BMD and osteoporotic cells: p = 0.04; osteopenic cells: p = 0.006).

Conclusions

These findings suggest that HBO therapy holds potential as an adjunctive or investigational treatment for elderly patients to enhance bone density or facilitate bone healing post-fracture, especially in those with normal or osteopenic bone density. This approach could potentially influence clinical practices in the future.

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来源期刊

Journal of orthopaedics ORTHOPEDICS-

CiteScore

3.50

自引率

6.70%

发文量

202

审稿时长

56 days

期刊介绍： Journal of Orthopaedics aims to be a leading journal in orthopaedics and contribute towards the improvement of quality of orthopedic health care. The journal publishes original research work and review articles related to different aspects of orthopaedics including Arthroplasty, Arthroscopy, Sports Medicine, Trauma, Spine and Spinal deformities, Pediatric orthopaedics, limb reconstruction procedures, hand surgery, and orthopaedic oncology. It also publishes articles on continuing education, health-related information, case reports and letters to the editor. It is requested to note that the journal has an international readership and all submissions should be aimed at specifying something about the setting in which the work was conducted. Authors must also provide any specific reasons for the research and also provide an elaborate description of the results.