Hanaa S S Gazwi, Amany E Ragab, Osama I A Soltan, Mohamed A Abdein, Bushra Shakoor, Nusrat Shafiq, Inas Hussein Refaat, Salim S Al-Rejaie, Sinisa Djurasevic, Mohamed Mohany, Asmaa H Zaki
{"title":"刺果提取物对CCl4致肾损伤的保护作用。","authors":"Hanaa S S Gazwi, Amany E Ragab, Osama I A Soltan, Mohamed A Abdein, Bushra Shakoor, Nusrat Shafiq, Inas Hussein Refaat, Salim S Al-Rejaie, Sinisa Djurasevic, Mohamed Mohany, Asmaa H Zaki","doi":"10.1016/j.yrtph.2025.105913","DOIUrl":null,"url":null,"abstract":"<p><p>The study explored the nephroprotective potential of Rosa canina (dog rose) ethanolic fruit extract against carbon tetrachloride (CCl4)-induced nephrotoxicity in rats, while also analyzing its phytochemical composition using UPLC-ESI-MS/MS. Male Wistar rats were allocated into five groups: control, R. canina extract alone, CCl4-induced nephrotoxicity, CCl4 with R. canina extract and CCl4 with silymarin. UPLC-ESI-MS/MS revealed 15 compounds in R. canina extract, predominantly anthocyanins, flavonoids, and lycopene. Treatment with R. canina extract significantly ameliorated CCl4-induced kidney dysfunction, abating oxidative stress and inflammation. Enhanced expression of HO-1 (heme oxygenase-1) and Nrf2 (nuclear factor erythroid 2-related factor 2) mRNA in the kidney suggested their involvement in protective mechanisms. Inhibition of HO-1 attenuated R. canina's protective effect against CCl4-induced kidney injury, underscoring the significance of the Nrf2/HO-1 pathway. For further validation, high throughput molecular docking analysis were performed. The docking analysis revealed the interaction between HO-1 and Nrf2 against Pelarginidin, Malvidin and Petunidin. Among all the three compounds, pelargonidin showed the highest binding score of -9.3kcal/mol and -7.7kcal/mol against Nrf2 and HO-1 respectively. In conclusion, R. canina extract, rich in phenolics, exhibited nephroprotective effects via inflammation and oxidative stress attenuation, potentially mediated through Nrf2/HO-1 pathway modulation against CCl4-induced nephrotoxicity.</p>","PeriodicalId":20852,"journal":{"name":"Regulatory Toxicology and Pharmacology","volume":" ","pages":"105913"},"PeriodicalIF":3.0000,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Protective Effects of Rosa canina Fruit Extract against Kidney Damage Induced by CCl4.\",\"authors\":\"Hanaa S S Gazwi, Amany E Ragab, Osama I A Soltan, Mohamed A Abdein, Bushra Shakoor, Nusrat Shafiq, Inas Hussein Refaat, Salim S Al-Rejaie, Sinisa Djurasevic, Mohamed Mohany, Asmaa H Zaki\",\"doi\":\"10.1016/j.yrtph.2025.105913\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The study explored the nephroprotective potential of Rosa canina (dog rose) ethanolic fruit extract against carbon tetrachloride (CCl4)-induced nephrotoxicity in rats, while also analyzing its phytochemical composition using UPLC-ESI-MS/MS. Male Wistar rats were allocated into five groups: control, R. canina extract alone, CCl4-induced nephrotoxicity, CCl4 with R. canina extract and CCl4 with silymarin. UPLC-ESI-MS/MS revealed 15 compounds in R. canina extract, predominantly anthocyanins, flavonoids, and lycopene. Treatment with R. canina extract significantly ameliorated CCl4-induced kidney dysfunction, abating oxidative stress and inflammation. Enhanced expression of HO-1 (heme oxygenase-1) and Nrf2 (nuclear factor erythroid 2-related factor 2) mRNA in the kidney suggested their involvement in protective mechanisms. Inhibition of HO-1 attenuated R. canina's protective effect against CCl4-induced kidney injury, underscoring the significance of the Nrf2/HO-1 pathway. For further validation, high throughput molecular docking analysis were performed. The docking analysis revealed the interaction between HO-1 and Nrf2 against Pelarginidin, Malvidin and Petunidin. 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Protective Effects of Rosa canina Fruit Extract against Kidney Damage Induced by CCl4.
The study explored the nephroprotective potential of Rosa canina (dog rose) ethanolic fruit extract against carbon tetrachloride (CCl4)-induced nephrotoxicity in rats, while also analyzing its phytochemical composition using UPLC-ESI-MS/MS. Male Wistar rats were allocated into five groups: control, R. canina extract alone, CCl4-induced nephrotoxicity, CCl4 with R. canina extract and CCl4 with silymarin. UPLC-ESI-MS/MS revealed 15 compounds in R. canina extract, predominantly anthocyanins, flavonoids, and lycopene. Treatment with R. canina extract significantly ameliorated CCl4-induced kidney dysfunction, abating oxidative stress and inflammation. Enhanced expression of HO-1 (heme oxygenase-1) and Nrf2 (nuclear factor erythroid 2-related factor 2) mRNA in the kidney suggested their involvement in protective mechanisms. Inhibition of HO-1 attenuated R. canina's protective effect against CCl4-induced kidney injury, underscoring the significance of the Nrf2/HO-1 pathway. For further validation, high throughput molecular docking analysis were performed. The docking analysis revealed the interaction between HO-1 and Nrf2 against Pelarginidin, Malvidin and Petunidin. Among all the three compounds, pelargonidin showed the highest binding score of -9.3kcal/mol and -7.7kcal/mol against Nrf2 and HO-1 respectively. In conclusion, R. canina extract, rich in phenolics, exhibited nephroprotective effects via inflammation and oxidative stress attenuation, potentially mediated through Nrf2/HO-1 pathway modulation against CCl4-induced nephrotoxicity.
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Regulatory Toxicology and Pharmacology publishes peer reviewed articles that involve the generation, evaluation, and interpretation of experimental animal and human data that are of direct importance and relevance for regulatory authorities with respect to toxicological and pharmacological regulations in society. All peer-reviewed articles that are published should be devoted to improve the protection of human health and environment. Reviews and discussions are welcomed that address legal and/or regulatory decisions with respect to risk assessment and management of toxicological and pharmacological compounds on a scientific basis. It addresses an international readership of scientists, risk assessors and managers, and other professionals active in the field of human and environmental health.
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