呼气冷凝物中的microrna：结核病诊断的新型非侵入性生物标志物

IF 2.9 3区医学 Q3 IMMUNOLOGY

Tuberculosis Pub Date : 2025-07-02 DOI:10.1016/j.tube.2025.102670

Tak Jaya , Pattnaik Bijay , Rai Divyanjali , Bhatraju Naveen , P.P. Adwika , Bangaru Sunil , Sunita Yadav , Sachin kumar , Seetu Kumari , Umashankar Verma , Geetika Yadav , R.S. Dhaliwal , Saurabh Mittal , Pawan Tiwari , Vijay Hadda , Karan Madan , Anurag Agrawal , Randeep Guleria , Tapasya Srivastava , Anant Mohan

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引用次数: 0

摘要

结核病仍然是一个重大的全球卫生问题。痰涂片镜检灵敏度低，难以诊断。对EBC（呼出液）miRNA的分析提供了一种潜在的非侵入性诊断方法，可以提高结核病检测的敏感性和特异性，包括肺外结核（EPTB）。方法收集65例naïve结核治疗患者和65例健康对照者的debc。使用基于qRT-PCR的miRNA分析试剂盒在一组探索性集合（n = 40）上进行miRNA分析，并使用qRT-PCR在另一组（n = 25）中验证入围的miRNA。ROC曲线用于评价诊断效果。结果在本研究中，我们鉴定出结核患者与健康受试者中8个差异表达的mirna（7个上调，1个下调）。与EPTB相比，PTB中有5个mirna上调，68个mirna下调。在PTB和健康对照组之间，PTB中有6个mirna上调，16个mirna下调，而EPTB中有132个mirna上调。验证证实miR-454、miR-139和miR-143在结核中上调。我们的研究结果表明，miR-143、miR-454和miR-139在呼出液中的表达具有很强的潜力，可以作为结核病诊断的非侵入性生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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MicroRNAs in exhaled breath Condensate: Novel non-invasive biomarkers for tuberculosis diagnosis

Background

Tuberculosis remains a significant global health issue. Sputum smear microscopy has low sensitivity, making it difficult to diagnose. Analysis of miRNA from EBC (exhaled breath condensate) offers a potential non-invasive diagnostic method that could improve sensitivity and specificity for TB detection, including extrapulmonary TB (EPTB).

Method

EBC was collected from 65 treatment naïve TB patients and 65 healthy controls. miRNA profiling was done on an exploratory set (n = 40) using the qRT-PCR-based miRNome profiler kit, and shortlisted miRNAs were validated in a separate set (n = 25) using qRT-PCR. ROC curves were used to evaluate diagnostic performance.

Result

In this study, we identified eight differentially expressed miRNAs in TB vs healthy subjects (seven upregulated, one downregulated). Comparing PTB with EPTB, five miRNAs were upregulated and 68 miRNAs were downregulated in PTB. Between PTB and healthy controls, six miRNAs were upregulated and 16 miRNAs were downregulated in PTB, while in EPTB, 132 miRNAs were upregulated compared with controls. Validation confirmed upregulation of miR-454, miR-139, and miR-143 in tuberculosis.

Conclusion

The findings of our study indicate that the expression of miR-143, miR-454, and miR-139 in exhaled breath condensate has strong potential as a non-invasive biomarker for TB diagnosis.

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来源期刊

Tuberculosis 医学-呼吸系统

CiteScore

4.60

自引率

3.10%

发文量

审稿时长

49 days

期刊介绍： Tuberculosis is a speciality journal focusing on basic experimental research on tuberculosis, notably on bacteriological, immunological and pathogenesis aspects of the disease. The journal publishes original research and reviews on the host response and immunology of tuberculosis and the molecular biology, genetics and physiology of the organism, however discourages submissions with a meta-analytical focus (for example, articles based on searches of published articles in public electronic databases, especially where there is lack of evidence of the personal involvement of authors in the generation of such material). We do not publish Clinical Case-Studies. Areas on which submissions are welcomed include: -Clinical TrialsDiagnostics- Antimicrobial resistance- Immunology- Leprosy- Microbiology, including microbial physiology- Molecular epidemiology- Non-tuberculous Mycobacteria- Pathogenesis- Pathology- Vaccine development. This Journal does not accept case-reports. The resurgence of interest in tuberculosis has accelerated the pace of relevant research and Tuberculosis has grown with it, as the only journal dedicated to experimental biomedical research in tuberculosis.