多工具海洋元条形码生物评价在海带生境的基线和监测物种和群落。

IF 5.5 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Giulia Maiello, Marilla R Lippert, Erika F Neave, Erik A Hanson, Stephen R Palumbi, Stefano Mariani
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引用次数: 0

摘要

加州海带林中惊人的生物多样性需要有效和强大的监测工具,以更好地了解生态趋势,并减轻由于人类压力和气候变化而导致的生态系统服务丧失或中断。随着环境DNA (eDNA)元条形码成为一种流行的生物多样性评估方法,我们开始评估强大、快速和可持续的eDNA解决方案的组合,以表征加利福尼亚中部海岸以海带为主的栖息地的海洋群落组成,在新提议的丘马什遗产国家海洋保护区。我们采用并比较了几种eDNA收集方法的效率,包括“传统的”地表水过滤,收集鹅卵石岩石上的生物和人工被动采样器的各种部署,即元探针(即附着在潜水员身上,悬挂在船上,用鱼竿从岸上抛下)。通过结合鱼类特异性(Tele02 12S)和普遍后生动物(COI)标记的信息,我们“捕获”了501个独特的海洋分类群,属于至少36门,其中400多个被鉴定为属/种水平,其中包括52种典型的加利福尼亚海带森林生态系统脊椎动物。尽管不同采样方法所返回的生物多样性类型存在差异,但调查地区的整体群落结构具有高度的空间结构,并受到Humqaq点附近生物地理断裂的强烈影响。我们讨论了将eDNA元条形码整合到现有监测程序中的好处,并设计了一种可重复的方法来监测海带林栖息地及其他地区的动物变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Multi-Tool Marine Metabarcoding Bioassessment for Baselining and Monitoring Species and Communities in Kelp Habitats.

The astonishing biological diversity found in Californian kelp forests requires efficient and robust monitoring tools to better understand ecological trends and mitigate against loss or disruption of ecosystem services due to human pressure and climate changes. With environmental DNA (eDNA) metabarcoding becoming a popular biodiversity assessment approach, we set out to evaluate a combination of powerful, rapid and sustainable eDNA solutions for characterising marine community composition in kelp-dominated habitats along the central California coast, in the newly proposed Chumash Heritage National Marine Sanctuary. We employed and compared the efficiency of several eDNA collection approaches, including 'traditional' surface water filtration, the collection of organisms encrusting cobble rocks and various deployments of an artificial passive sampler, the metaprobe (i.e., attached to divers, dangled from a boat and cast from the shore using a fishing rod). By combining the information from fish specific (Tele02 12S) and universal metazoan (COI) markers, we 'captured' 501 unique marine taxa, belonging to at least 36 phyla, over 400 of which were identified to genus/species level, and including 52 vertebrate species typical of Californian kelp forest ecosystems. Despite differences in the type of biodiversity returned by the tested sampling methods, the overall community structure of the surveyed area was highly spatially structured and strongly influenced by the biogeographic break around Point Conception (Humqaq). We discuss the benefits of integrating eDNA metabarcoding in existing monitoring programs and devising a reproducible approach to monitor faunal changes in kelp forest habitats and beyond.

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来源期刊
Molecular Ecology Resources
Molecular Ecology Resources 生物-进化生物学
CiteScore
15.60
自引率
5.20%
发文量
170
审稿时长
3 months
期刊介绍: Molecular Ecology Resources promotes the creation of comprehensive resources for the scientific community, encompassing computer programs, statistical and molecular advancements, and a diverse array of molecular tools. Serving as a conduit for disseminating these resources, the journal targets a broad audience of researchers in the fields of evolution, ecology, and conservation. Articles in Molecular Ecology Resources are crafted to support investigations tackling significant questions within these disciplines. In addition to original resource articles, Molecular Ecology Resources features Reviews, Opinions, and Comments relevant to the field. The journal also periodically releases Special Issues focusing on resource development within specific areas.
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