New Pyrazole-Based Tetrazole Derivatives: Synthesis, Characterization, and Their Vasorelaxant and α-Amylase Inhibition Activities

A series of new linear pyrazole-based tetrazole derivatives 1–10 were synthesized and characterized. The structures of the intermediate compounds were confirmed using ¹H and ¹³C NMR spectroscopy, as well as high-resolution mass spectrometry (HRMS). These derivatives were synthesized through a straightforward equimolar condensation between pyrazole and tetrazole precursors. The vasorelaxant activity of these compounds was assessed by determining their percentage inhibition, E (%), which ranged from 26% to 67%, with compound 4 exhibiting the highest activity. Additionally, their anti-diabetic potential was evaluated by determining the IC₅₀ values for α-amylase enzyme inhibition. Notably, compounds 2 and 6 demonstrated a comparable activity to the positive control acarbose. These experimental findings were further supported by molecular docking studies.