LncRNA STEAP3-AS1通过染色质重塑调节组蛋白乳酸化,促进结直肠癌肝转移。

IF 12.8 1区 医学 Q1 ONCOLOGY
Jinjuan Lv, Xiaoqi Yu, Xiaoqian Liu, Qianshi Zhang, Mengyan Zhang, Jianfeng Gao, Zhiwei Sun, Feifan Zhang, Yunfei Zuo, Shuangyi Ren
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引用次数: 0

摘要

肝转移是结直肠癌(CRC)患者死亡的常见原因;然而,其分子机制尚不清楚。在此,我们旨在揭示lncRNA STEAP3-AS1在调节染色质重塑和组蛋白乳酸化中的作用,探讨lncRNA STEAP3-AS1促进结直肠癌肝转移的机制。本研究为肿瘤的临床治疗提供了新的研究思路和理论依据。方法:本研究采用CRC类器官和裸鼠肝转移模型,分析lncRNA STEAP3-AS1对CRC肝转移的影响。采用ATAC-seq、RNA-seq、DRIP-seq和Western blotting筛选lncRNA STEAP3-AS1下游前转移分子MMP9和染色质重塑因子BRG1。通过Co-IP评估BRG1、p300和HDAC3之间的蛋白相互作用。采用ChIP-qPCR检测BRG1、ERG、P300和H3K18la与MMP9基因启动子的结合情况。结果:lncRNA STEAP3- as1与其亲本基因STEAP3相互作用,在关键染色质重塑因子BRG1上形成r环,调控BRG1的表达。进一步的证据表明,BRG1与组蛋白乳酸化擦除者HDAC3和书写者P300形成蛋白复合物,调节H3K18la的表达。此外,ATAC-seq分析显示,lncRNA STEAP3-AS1促进MMP9的染色质可及性,基序和数据库分析确定肿瘤转移因子ERG是MMP9的转录因子。lncRNA STEAP3-AS1介导BRG1/ERG/P300复合物对H3K18la活化MMP9的调控。结论:综上所述,这些发现揭示了lncRNA STEAP3- as1与其亲本基因STEAP3相互作用,通过BRG1调控H3K18la,导致染色质可及性改变,从而驱动ERG富集和MMP9启动子激活MMP9转录,促进结直肠癌肝转移。我们的研究结果从表观遗传学角度揭示了lncRNA STEAP3-AS1促进结直肠癌转移的新机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The LncRNA STEAP3-AS1 promotes liver metastasis in colorectal cancer by regulating histone lactylation through chromatin remodelling.

Introduction: Liver metastasis is a common cause of death in patients with colorectal cancer (CRC); however, its molecular mechanism remains unclear. Here, we aimed to reveal the role of the lncRNA STEAP3-AS1 in regulating chromatin remodelling and histone lactylation and explore the mechanism by which the lncRNA STEAP3-AS1 promotes CRC liver metastasis. This study provide new research ideas and a theoretical basis for the clinical treatment of cancer.

Methods: In this study, we used CRC organoid and nude mouse liver metastasis models to analyse the effect of the lncRNA STEAP3-AS1 on CRC liver metastasis. ATAC-seq, RNA-seq, DRIP-seq and Western blotting were used to screen for the lncRNA STEAP3-AS1 downstream prometastatic molecule MMP9 and the chromatin remodelling factor BRG1. The protein interactions between BRG1, p300, and HDAC3 were evaluated by Co-IP. The the binding of BRG1, ERG, P300, and H3K18la to the MMP9 gene promoter was detected using ChIP-qPCR.

Results: The lncRNA STEAP3-AS1 interacts with its parental gene, STEAP3, to form an R-loop on the key chromatin remodelling factor BRG1, regulating the expression of BRG1. Further evidence suggests that BRG1 forms a protein complex with the histone lactylation eraser HDAC3 and the writer P300 to regulate the expression of H3K18la. Moreover, the ATAC-seq analysis revealed that the lncRNA STEAP3-AS1 promotes the chromatin accessibility of MMP9, and a motif and database analysis identified the tumour metastasis factor ERG as an MMP9 transcription factor. The lncRNA STEAP3-AS1 mediates regulation of H3K18la activation of MMP9 by the BRG1/ERG/P300 complex.

Conclusions: In summary, these findings revealed that the lncRNA STEAP3-AS1 interacts with its parental gene STEAP3 to regulate H3K18la through BRG1, resulting in changes in chromatin accessibility, thereby driving ERG enrichment an the MMP9 promoter to activate MMP9 transcription and promote CRC liver metastasis. Our findings reveal a novel mechanism by which the lncRNA STEAP3-AS1 promotes CRC metastasis from an epigenetic perspective.

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来源期刊
CiteScore
18.20
自引率
1.80%
发文量
333
审稿时长
1 months
期刊介绍: The Journal of Experimental & Clinical Cancer Research is an esteemed peer-reviewed publication that focuses on cancer research, encompassing everything from fundamental discoveries to practical applications. We welcome submissions that showcase groundbreaking advancements in the field of cancer research, especially those that bridge the gap between laboratory findings and clinical implementation. Our goal is to foster a deeper understanding of cancer, improve prevention and detection strategies, facilitate accurate diagnosis, and enhance treatment options. We are particularly interested in manuscripts that shed light on the mechanisms behind the development and progression of cancer, including metastasis. Additionally, we encourage submissions that explore molecular alterations or biomarkers that can help predict the efficacy of different treatments or identify drug resistance. Translational research related to targeted therapies, personalized medicine, tumor immunotherapy, and innovative approaches applicable to clinical investigations are also of great interest to us. We provide a platform for the dissemination of large-scale molecular characterizations of human tumors and encourage researchers to share their insights, discoveries, and methodologies with the wider scientific community. By publishing high-quality research articles, reviews, and commentaries, the Journal of Experimental & Clinical Cancer Research strives to contribute to the continuous improvement of cancer care and make a meaningful impact on patients' lives.
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