秀丽隐杆线虫分泌腺细胞和NSPC基因的生理作用研究。

IF 5.1 3区 生物学 Q2 GENETICS & HEREDITY
Genetics Pub Date : 2025-09-03 DOI:10.1093/genetics/iyaf125
Zuzanna Mackiewicz, Vladyslava Liudkovska, Andrzej Dziembowski
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引用次数: 0

摘要

秀丽隐杆线虫是分子生物学中研究最多的模式生物之一;然而,其生理学的许多方面和许多基因的功能仍然知之甚少。在这项研究中,我们研究了线虫特异性NSPC蛋白的作用,其mrna最近被鉴定为poly(A)聚合酶TENT-5的主要靶点。令人惊讶的是,我们发现NSPCs仅在排泄腺细胞中表达,而排泄腺细胞的功能尚不清楚。利用光遗传学方法,我们精确地切除了排泄腺细胞,并观察到,除了NSPCs的强烈下调外,线虫在没有NSPCs的情况下没有表现出转录组学或生理变化。此外,我们生成并彻底研究了一个缺失所有18个nspc基因的菌株,这表明,尽管先前的迹象表明,nspc不会影响蠕虫的防御反应。相反,转录组学分析显示,NSPCs的缺失导致了与DAF-16突变体相似的基因表达变化,这表明NSPCs可能以某种方式影响了秀丽隐杆线虫的关键胰岛素信号通路。虽然需要进一步的研究来解释确切的遗传相互作用并阐明其生理效应,但我们的发现为线虫生理学的这一未被探索的部分提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Excretory gland cell and NSPC genes in C. elegans: investigating their physiological roles.

The nematode Caenorhabditis elegans is one of the best-studied model organisms in molecular biology; however, many aspects of its physiology and the functions of many genes remain poorly understood. In this study, we investigated the role of Nematode-Specific Peptide Family, group C (NSPC) proteins, whose mRNAs were recently identified as primary targets of the poly(A) polymerase TENT-5. Surprisingly, we found that NSPCs are exclusively expressed in the excretory gland cell, a cell with still unclear functionality. Using an optogenetic approach, we precisely ablated the excretory gland cell and observed that, apart from a strong downregulation of NSPCs, nematodes exhibited no transcriptomic or physiological changes in its absence. Additionally, we generated and thoroughly studied a strain with a deletion of all 18 nspc genes, which revealed that, despite previous indications, NSPCs do not influence the worm's defense response. Instead, the transcriptomic analysis showed that the absence of NSPCs results in gene expression changes resembling those observed for DAF-16 mutants, suggesting that NSPCs may somehow influence the key C. elegans insulin signaling pathway. Although further studies are required to explain the exact genetic interaction and elucidate its physiological effects, our findings provide new insights into this unexplored part of nematode physiology.

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来源期刊
Genetics
Genetics GENETICS & HEREDITY-
CiteScore
6.90
自引率
6.10%
发文量
177
审稿时长
1.5 months
期刊介绍: GENETICS is published by the Genetics Society of America, a scholarly society that seeks to deepen our understanding of the living world by advancing our understanding of genetics. Since 1916, GENETICS has published high-quality, original research presenting novel findings bearing on genetics and genomics. The journal publishes empirical studies of organisms ranging from microbes to humans, as well as theoretical work. While it has an illustrious history, GENETICS has changed along with the communities it serves: it is not your mentor''s journal. The editors make decisions quickly – in around 30 days – without sacrificing the excellence and scholarship for which the journal has long been known. GENETICS is a peer reviewed, peer-edited journal, with an international reach and increasing visibility and impact. All editorial decisions are made through collaboration of at least two editors who are practicing scientists. GENETICS is constantly innovating: expanded types of content include Reviews, Commentary (current issues of interest to geneticists), Perspectives (historical), Primers (to introduce primary literature into the classroom), Toolbox Reviews, plus YeastBook, FlyBook, and WormBook (coming spring 2016). For particularly time-sensitive results, we publish Communications. As part of our mission to serve our communities, we''ve published thematic collections, including Genomic Selection, Multiparental Populations, Mouse Collaborative Cross, and the Genetics of Sex.
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