Sylwia M Czukiewska, Felicitas Azpiroz, Susana M Chuva de Sousa Lopes, Mina Popovic
{"title":"人类胚胎体外扩展培养。","authors":"Sylwia M Czukiewska, Felicitas Azpiroz, Susana M Chuva de Sousa Lopes, Mina Popovic","doi":"10.1007/7651_2025_641","DOIUrl":null,"url":null,"abstract":"<p><p>Recent innovations in extended in vitro culture (IVC) systems have revolutionized our understanding of human peri-implantation development. Building on foundational animal studies, refined protocols now support human embryo culture beyond the blastocyst stage, providing unprecedented access to previously elusive developmental events. These systems have yielded critical insights into early morphogenetic processes, lineage specification, and tissue organization, significantly advancing developmental biology. Here, we provide our current protocol for the extended culture of human embryos, followed by immunofluorescence for lineage markers of interest. Unveiling human peri-implantation development also promises to improve reproductive medicine, potentially addressing challenges related to implantation failure, chromosomal instability in embryos, as well as congenital disorders. Insights gained from this research may pave way for novel therapeutic approaches and advancements in medically assisted reproduction.</p>","PeriodicalId":18490,"journal":{"name":"Methods in molecular biology","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Extended In Vitro Culture of Human Embryos.\",\"authors\":\"Sylwia M Czukiewska, Felicitas Azpiroz, Susana M Chuva de Sousa Lopes, Mina Popovic\",\"doi\":\"10.1007/7651_2025_641\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Recent innovations in extended in vitro culture (IVC) systems have revolutionized our understanding of human peri-implantation development. Building on foundational animal studies, refined protocols now support human embryo culture beyond the blastocyst stage, providing unprecedented access to previously elusive developmental events. These systems have yielded critical insights into early morphogenetic processes, lineage specification, and tissue organization, significantly advancing developmental biology. Here, we provide our current protocol for the extended culture of human embryos, followed by immunofluorescence for lineage markers of interest. Unveiling human peri-implantation development also promises to improve reproductive medicine, potentially addressing challenges related to implantation failure, chromosomal instability in embryos, as well as congenital disorders. Insights gained from this research may pave way for novel therapeutic approaches and advancements in medically assisted reproduction.</p>\",\"PeriodicalId\":18490,\"journal\":{\"name\":\"Methods in molecular biology\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-07-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Methods in molecular biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1007/7651_2025_641\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Methods in molecular biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/7651_2025_641","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
Recent innovations in extended in vitro culture (IVC) systems have revolutionized our understanding of human peri-implantation development. Building on foundational animal studies, refined protocols now support human embryo culture beyond the blastocyst stage, providing unprecedented access to previously elusive developmental events. These systems have yielded critical insights into early morphogenetic processes, lineage specification, and tissue organization, significantly advancing developmental biology. Here, we provide our current protocol for the extended culture of human embryos, followed by immunofluorescence for lineage markers of interest. Unveiling human peri-implantation development also promises to improve reproductive medicine, potentially addressing challenges related to implantation failure, chromosomal instability in embryos, as well as congenital disorders. Insights gained from this research may pave way for novel therapeutic approaches and advancements in medically assisted reproduction.
期刊介绍：
For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
