{"title":"印楝甲醇叶提取物通过调节氧化应激、PPARα和BCL2信号通路减轻异丙肾上腺素诱导的大鼠心肌梗死。","authors":"Amirah Folashade Yusuf, Temitayo Olabisi Ajibade, Oluwaseun Olarenwaju Esan, Racheal Ebunoluwa Asenuga, Moyinoluwa Onoja, Matthew Obot Akpan, Joseph Ayotunde Badejo, Temidayo Olutayo Omobowale, Ademola Adetokunbo Oyagbemi, Adeolu Alex Adedapo, Oluwafemi Omoniyi Oguntibeju, Momoh Audu Yakubu","doi":"10.22038/ajp.2024.25277","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Evaluation of <i>Azadirachta indica</i>'s potential on the modulation of blood pressure parameters, antioxidant defense status, as well as immunohistochemical expressions of Peroxisome proliferator-activated receptor α (PPARα) and Bcl-2 (B-cell lymphoma 2) in rats exposed to isoproterenol was the objective of this study.</p><p><strong>Materials and methods: </strong>Fifty rats (<i>Rattus norvegicus</i>) of the Wistar strain were used, with myocardial infarction induced by intraperitoneal administration of isoproterenol (ISO) for two consecutive days. Cardiac and renal biomarkers of oxidative stress, blood pressure parameters, electrocardiography, and immunohistochemical staining of PPARα and BCL2 were performed.</p><p><strong>Results: </strong>ISO toxicity heightened blood pressure parameters, aggravated oxidative processes, declined antioxidant defense system, and decreased immunohistochemical expressions of PPARα and BCL2. Interestingly, <i>A. indica</i> improved antioxidant status, lowered free radical generation, mitigated serum myeloperoxidase and xanthine oxidase activities, respectively.</p><p><strong>Conclusion: </strong>Mitigation of oxidative mechanisms and antihypertensive effects of <i>Azadirachta indica</i> suggest a positive modulatory role for the medicinal plant in isoproterenol-induced myocardial infarction.</p>","PeriodicalId":8677,"journal":{"name":"Avicenna Journal of Phytomedicine","volume":"15 4","pages":"1328-1340"},"PeriodicalIF":1.9000,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12244948/pdf/","citationCount":"0","resultStr":"{\"title\":\"Methanol leaf extract of <i>Azadirachta</i> <i>indica</i> mitigates isoproterenol-induced myocardial infarction through the modulation of oxidative stress, and PPARα and BCL2 signaling in rats.\",\"authors\":\"Amirah Folashade Yusuf, Temitayo Olabisi Ajibade, Oluwaseun Olarenwaju Esan, Racheal Ebunoluwa Asenuga, Moyinoluwa Onoja, Matthew Obot Akpan, Joseph Ayotunde Badejo, Temidayo Olutayo Omobowale, Ademola Adetokunbo Oyagbemi, Adeolu Alex Adedapo, Oluwafemi Omoniyi Oguntibeju, Momoh Audu Yakubu\",\"doi\":\"10.22038/ajp.2024.25277\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>Evaluation of <i>Azadirachta indica</i>'s potential on the modulation of blood pressure parameters, antioxidant defense status, as well as immunohistochemical expressions of Peroxisome proliferator-activated receptor α (PPARα) and Bcl-2 (B-cell lymphoma 2) in rats exposed to isoproterenol was the objective of this study.</p><p><strong>Materials and methods: </strong>Fifty rats (<i>Rattus norvegicus</i>) of the Wistar strain were used, with myocardial infarction induced by intraperitoneal administration of isoproterenol (ISO) for two consecutive days. Cardiac and renal biomarkers of oxidative stress, blood pressure parameters, electrocardiography, and immunohistochemical staining of PPARα and BCL2 were performed.</p><p><strong>Results: </strong>ISO toxicity heightened blood pressure parameters, aggravated oxidative processes, declined antioxidant defense system, and decreased immunohistochemical expressions of PPARα and BCL2. Interestingly, <i>A. indica</i> improved antioxidant status, lowered free radical generation, mitigated serum myeloperoxidase and xanthine oxidase activities, respectively.</p><p><strong>Conclusion: </strong>Mitigation of oxidative mechanisms and antihypertensive effects of <i>Azadirachta indica</i> suggest a positive modulatory role for the medicinal plant in isoproterenol-induced myocardial infarction.</p>\",\"PeriodicalId\":8677,\"journal\":{\"name\":\"Avicenna Journal of Phytomedicine\",\"volume\":\"15 4\",\"pages\":\"1328-1340\"},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2025-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12244948/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Avicenna Journal of Phytomedicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.22038/ajp.2024.25277\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, MEDICINAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Avicenna Journal of Phytomedicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22038/ajp.2024.25277","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}
Methanol leaf extract of Azadirachtaindica mitigates isoproterenol-induced myocardial infarction through the modulation of oxidative stress, and PPARα and BCL2 signaling in rats.
Objective: Evaluation of Azadirachta indica's potential on the modulation of blood pressure parameters, antioxidant defense status, as well as immunohistochemical expressions of Peroxisome proliferator-activated receptor α (PPARα) and Bcl-2 (B-cell lymphoma 2) in rats exposed to isoproterenol was the objective of this study.
Materials and methods: Fifty rats (Rattus norvegicus) of the Wistar strain were used, with myocardial infarction induced by intraperitoneal administration of isoproterenol (ISO) for two consecutive days. Cardiac and renal biomarkers of oxidative stress, blood pressure parameters, electrocardiography, and immunohistochemical staining of PPARα and BCL2 were performed.
Results: ISO toxicity heightened blood pressure parameters, aggravated oxidative processes, declined antioxidant defense system, and decreased immunohistochemical expressions of PPARα and BCL2. Interestingly, A. indica improved antioxidant status, lowered free radical generation, mitigated serum myeloperoxidase and xanthine oxidase activities, respectively.
Conclusion: Mitigation of oxidative mechanisms and antihypertensive effects of Azadirachta indica suggest a positive modulatory role for the medicinal plant in isoproterenol-induced myocardial infarction.
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