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引用次数: 0
摘要
最近有研究表明，从鱼类到原始哺乳动物，脊椎动物都具有IV型IFN （IFNυ）。然而，它们的精确功能和复杂的信号仍然未知。本研究从异种鲫鱼（Carassius gibelio）中鉴定到CaIFNυ，并对其转录调控和信号转导进行了进一步的研究。首先，与来自C. gibelio的典型I型IFN CaIFNa1相比，CaIFNυ在抗病毒特性的分布、大小和动力学上存在显著差异。其次，IRF1/3/7通过同源或异源蛋白复合物以剂量依赖性的方式差异激活CaIFNυ， NF-κ b （p65）是最有效的刺激剂。第三，CaIFNυ通过与CaCRFB4和CaCRFB12的细胞外区域结合，通过JAK-STAT以及MAPK和PI3K信号通路启动抗病毒ISGs。综上所述，这些发现表明CaIFNυ属于抗病毒细胞因子谱系，与CaIFNa1不同。
Transcriptional regulation and signaling of type IV interferon in Carassius gibelio.
It was recently shown that vertebrates, ranging from fish to primitive mammals, possess type IV IFN (IFNυ). However, their precise function and elaborate signaling remains unknown. In this study, CaIFNυ was identified from Carassius gibelio and its transcriptional regulation and signaling was further investigated. Firstly, CaIFNυ displays significant differences in the distribution, magnitude, and kinetics of its antiviral properties compared with CaIFNa1, a typical type I IFN from C. gibelio. Secondly, IRF1/3/7 differentially activated CaIFNυ through homo- or heteroprotein complexes in a dose-dependent fashion and NF-κB (p65) was the most effective stimulator. Thirdly, CaIFNυ initiates the antiviral ISGs through the JAK-STAT as well as MAPK and PI3K signaling pathways by binding to the extra-cellular region of CaCRFB4 and CaCRFB12. Taken together, these findings reveal that CaIFNυ belonges to an antiviral cytokine lineage which is distinct from CaIFNa1.
期刊介绍：
Cell Communication and Signaling (CCS) is a peer-reviewed, open-access scientific journal that focuses on cellular signaling pathways in both normal and pathological conditions. It publishes original research, reviews, and commentaries, welcoming studies that utilize molecular, morphological, biochemical, structural, and cell biology approaches. CCS also encourages interdisciplinary work and innovative models, including in silico, in vitro, and in vivo approaches, to facilitate investigations of cell signaling pathways, networks, and behavior.
Starting from January 2019, CCS is proud to announce its affiliation with the International Cell Death Society. The journal now encourages submissions covering all aspects of cell death, including apoptotic and non-apoptotic mechanisms, cell death in model systems, autophagy, clearance of dying cells, and the immunological and pathological consequences of dying cells in the tissue microenvironment.
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