核受体过氧化物酶体增殖物激活受体γ (PPAR-γ)和Nrf2信号在甲醇诱导的大鼠脑、眼和胰腺毒性中的作用。

IF 3.4 3区 医学 Q2 NEUROSCIENCES
Meriam NN Rezk , Mariem Maher Shafek Keryakous , Michael A. Fawzy , Fatma El-Zahraa A. Abd El-Aziz , Asmaa F.A. Dawood , Hanan D. Yassa , Nermeen N. Welson
{"title":"核受体过氧化物酶体增殖物激活受体γ (PPAR-γ)和Nrf2信号在甲醇诱导的大鼠脑、眼和胰腺毒性中的作用。","authors":"Meriam NN Rezk ,&nbsp;Mariem Maher Shafek Keryakous ,&nbsp;Michael A. Fawzy ,&nbsp;Fatma El-Zahraa A. Abd El-Aziz ,&nbsp;Asmaa F.A. Dawood ,&nbsp;Hanan D. Yassa ,&nbsp;Nermeen N. Welson","doi":"10.1016/j.neuro.2025.07.003","DOIUrl":null,"url":null,"abstract":"<div><div>Toxic methyl alcohol is widely employed in industry, and it is highly toxic. Only 15 cc ingestion can result in irreversible blindness. The mechanism of toxicity is still a matter of debate. This study was conducted to investigate the incorporation of nuclear receptor peroxisome proliferator-activated receptor gamma (PPAR-γ) and Nrf2 signaling pathways in the toxicity of the brain, eye, and pancreas following ingestion of methyl alcohol and the possible protective role of PPAR-γ modulators. Twenty-four adult Wister albino rats were divided into four groups of six rats each: a control group, a pioglitazone group, a methanol group, and a combined pioglitazone and methanol group. Oxidative stress markers, random blood sugar, insulin, and pancreatic function measurements were evaluated. Western blot analysis for PPAR-γ and Nrf2 protein expressions was performed. Histopathological examination was performed for eye, brain, and pancreas tissues, and the results were compared. PPAR-γ seemed to be incorporated in the development of organ toxicity associated with methyl alcohol ingestion. The protective role of PPAR-γ modulators was achieved through the improvement of assessed pathways. Therefore, damage can be dramatically improved through incorporating PPAR-γ agonists in the management plan of methyl alcohol toxicity.</div></div>","PeriodicalId":19189,"journal":{"name":"Neurotoxicology","volume":"110 ","pages":"Pages 53-63"},"PeriodicalIF":3.4000,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The role of nuclear receptor peroxisome proliferator-activated receptor gamma (PPAR-γ) and Nrf2 signalling in methanol-induced on brain, eye, and pancreas toxicity in rats\",\"authors\":\"Meriam NN Rezk ,&nbsp;Mariem Maher Shafek Keryakous ,&nbsp;Michael A. Fawzy ,&nbsp;Fatma El-Zahraa A. Abd El-Aziz ,&nbsp;Asmaa F.A. Dawood ,&nbsp;Hanan D. Yassa ,&nbsp;Nermeen N. Welson\",\"doi\":\"10.1016/j.neuro.2025.07.003\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Toxic methyl alcohol is widely employed in industry, and it is highly toxic. Only 15 cc ingestion can result in irreversible blindness. The mechanism of toxicity is still a matter of debate. This study was conducted to investigate the incorporation of nuclear receptor peroxisome proliferator-activated receptor gamma (PPAR-γ) and Nrf2 signaling pathways in the toxicity of the brain, eye, and pancreas following ingestion of methyl alcohol and the possible protective role of PPAR-γ modulators. Twenty-four adult Wister albino rats were divided into four groups of six rats each: a control group, a pioglitazone group, a methanol group, and a combined pioglitazone and methanol group. Oxidative stress markers, random blood sugar, insulin, and pancreatic function measurements were evaluated. Western blot analysis for PPAR-γ and Nrf2 protein expressions was performed. Histopathological examination was performed for eye, brain, and pancreas tissues, and the results were compared. PPAR-γ seemed to be incorporated in the development of organ toxicity associated with methyl alcohol ingestion. The protective role of PPAR-γ modulators was achieved through the improvement of assessed pathways. Therefore, damage can be dramatically improved through incorporating PPAR-γ agonists in the management plan of methyl alcohol toxicity.</div></div>\",\"PeriodicalId\":19189,\"journal\":{\"name\":\"Neurotoxicology\",\"volume\":\"110 \",\"pages\":\"Pages 53-63\"},\"PeriodicalIF\":3.4000,\"publicationDate\":\"2025-07-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Neurotoxicology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0161813X25000920\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"NEUROSCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Neurotoxicology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0161813X25000920","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"NEUROSCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

有毒甲醇是工业上广泛使用的剧毒物质。只要摄入15cc就会导致不可逆的失明。毒性的机制仍然是一个有争议的问题。本研究旨在探讨核受体过氧化物酶体增殖体激活受体γ (PPAR-γ)和Nrf2信号通路在摄入甲醇后脑、眼和胰腺毒性中的掺入以及PPAR-γ调节剂可能的保护作用。将24只成年Wister白化大鼠分为4组,每组6只:对照组、吡格列酮组、甲醇组、吡格列酮与甲醇联合组。评估氧化应激标志物、随机血糖、胰岛素和胰腺功能测量。Western blot分析PPAR-γ和Nrf2蛋白的表达。分别行眼、脑、胰腺组织病理检查,比较结果。PPAR-γ似乎与摄入甲醇相关的器官毒性的发展有关。PPAR-γ调节剂的保护作用是通过改善评估的途径实现的。因此,通过在甲醇毒性管理计划中加入PPAR-γ激动剂可以显著改善损伤。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The role of nuclear receptor peroxisome proliferator-activated receptor gamma (PPAR-γ) and Nrf2 signalling in methanol-induced on brain, eye, and pancreas toxicity in rats
Toxic methyl alcohol is widely employed in industry, and it is highly toxic. Only 15 cc ingestion can result in irreversible blindness. The mechanism of toxicity is still a matter of debate. This study was conducted to investigate the incorporation of nuclear receptor peroxisome proliferator-activated receptor gamma (PPAR-γ) and Nrf2 signaling pathways in the toxicity of the brain, eye, and pancreas following ingestion of methyl alcohol and the possible protective role of PPAR-γ modulators. Twenty-four adult Wister albino rats were divided into four groups of six rats each: a control group, a pioglitazone group, a methanol group, and a combined pioglitazone and methanol group. Oxidative stress markers, random blood sugar, insulin, and pancreatic function measurements were evaluated. Western blot analysis for PPAR-γ and Nrf2 protein expressions was performed. Histopathological examination was performed for eye, brain, and pancreas tissues, and the results were compared. PPAR-γ seemed to be incorporated in the development of organ toxicity associated with methyl alcohol ingestion. The protective role of PPAR-γ modulators was achieved through the improvement of assessed pathways. Therefore, damage can be dramatically improved through incorporating PPAR-γ agonists in the management plan of methyl alcohol toxicity.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Neurotoxicology
Neurotoxicology 医学-毒理学
CiteScore
6.80
自引率
5.90%
发文量
161
审稿时长
70 days
期刊介绍: NeuroToxicology specializes in publishing the best peer-reviewed original research papers dealing with the effects of toxic substances on the nervous system of humans and experimental animals of all ages. The Journal emphasizes papers dealing with the neurotoxic effects of environmentally significant chemical hazards, manufactured drugs and naturally occurring compounds.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信