{"title":"利用小rna作为细菌合成转录后调节因子。","authors":"Jens Georg, Bork A Berghoff, Daniel Schindler","doi":"10.1021/acssynbio.5c00118","DOIUrl":null,"url":null,"abstract":"<p><p>Bacteria can respond to environmental changes by expressing small RNAs (sRNAs), which regulate mRNAs by complementary base-pairing. This regulatory mechanism allows bacteria to rapidly adapt their proteome. In recent years, sRNAs have gained attention as blueprints for synthetic regulators allowing control over user-defined targets. Multiple aspects need to be considered for efficient application of these versatile, on-demand, and easy-to-use tools. Advances in computational prediction and bioengineering concepts are the dawn of systematic synthetic sRNA biology. We provide an overview of sRNAs and alternative post-transcriptional regulators, highlight the requirements for successful regulation, and provide guidelines for design, construction, and sRNA application.</p>","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":" ","pages":"2405-2417"},"PeriodicalIF":3.7000,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12281614/pdf/","citationCount":"0","resultStr":"{\"title\":\"Harnessing Small RNAs as Synthetic Post-transcriptional Regulators in Bacteria.\",\"authors\":\"Jens Georg, Bork A Berghoff, Daniel Schindler\",\"doi\":\"10.1021/acssynbio.5c00118\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Bacteria can respond to environmental changes by expressing small RNAs (sRNAs), which regulate mRNAs by complementary base-pairing. This regulatory mechanism allows bacteria to rapidly adapt their proteome. In recent years, sRNAs have gained attention as blueprints for synthetic regulators allowing control over user-defined targets. Multiple aspects need to be considered for efficient application of these versatile, on-demand, and easy-to-use tools. Advances in computational prediction and bioengineering concepts are the dawn of systematic synthetic sRNA biology. We provide an overview of sRNAs and alternative post-transcriptional regulators, highlight the requirements for successful regulation, and provide guidelines for design, construction, and sRNA application.</p>\",\"PeriodicalId\":26,\"journal\":{\"name\":\"ACS Synthetic Biology\",\"volume\":\" \",\"pages\":\"2405-2417\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2025-07-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12281614/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Synthetic Biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1021/acssynbio.5c00118\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/7/8 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Synthetic Biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1021/acssynbio.5c00118","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/7/8 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Harnessing Small RNAs as Synthetic Post-transcriptional Regulators in Bacteria.
Bacteria can respond to environmental changes by expressing small RNAs (sRNAs), which regulate mRNAs by complementary base-pairing. This regulatory mechanism allows bacteria to rapidly adapt their proteome. In recent years, sRNAs have gained attention as blueprints for synthetic regulators allowing control over user-defined targets. Multiple aspects need to be considered for efficient application of these versatile, on-demand, and easy-to-use tools. Advances in computational prediction and bioengineering concepts are the dawn of systematic synthetic sRNA biology. We provide an overview of sRNAs and alternative post-transcriptional regulators, highlight the requirements for successful regulation, and provide guidelines for design, construction, and sRNA application.
期刊介绍：
The journal is particularly interested in studies on the design and synthesis of new genetic circuits and gene products; computational methods in the design of systems; and integrative applied approaches to understanding disease and metabolism.
Topics may include, but are not limited to:
Design and optimization of genetic systems
Genetic circuit design and their principles for their organization into programs
Computational methods to aid the design of genetic systems
Experimental methods to quantify genetic parts, circuits, and metabolic fluxes
Genetic parts libraries: their creation, analysis, and ontological representation
Protein engineering including computational design
Metabolic engineering and cellular manufacturing, including biomass conversion
Natural product access, engineering, and production
Creative and innovative applications of cellular programming
Medical applications, tissue engineering, and the programming of therapeutic cells
Minimal cell design and construction
Genomics and genome replacement strategies
Viral engineering
Automated and robotic assembly platforms for synthetic biology
DNA synthesis methodologies
Metagenomics and synthetic metagenomic analysis
Bioinformatics applied to gene discovery, chemoinformatics, and pathway construction
Gene optimization
Methods for genome-scale measurements of transcription and metabolomics
Systems biology and methods to integrate multiple data sources
in vitro and cell-free synthetic biology and molecular programming
Nucleic acid engineering.
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