组织低温电子断层扫描的创新：挑战和未来展望

IF 6.1 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current opinion in structural biology Pub Date : 2025-07-07 DOI:10.1016/j.sbi.2025.103112

Zhe Chen , Qiang Guo

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引用次数: 0

摘要

低温电子断层扫描（cryo-ET）正在革新单细胞标本的原位结构分析，但其在组织中的应用一直受到阻碍，主要是由于组织厚度带来的挑战。样品玻璃化、低温聚焦离子束（cryo-FIB）铣削和提升技术的进步大大改善了组织制备，使薄的、电子显微镜兼容的样品成为可能。此外，自动化、互补成像模式和人工智能的集成简化了成像工作流程和数据分析。这篇综述强调了这些技术的发展，它们对组织分析的影响，以及冷冻et在推进结构生物学和生物医学研究方面的未来潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Innovations in cryo-electron tomography for tissues: Challenges and future prospects

Cryo-electron tomography (cryo-ET) is revolutionizing in situ structural analysis of single-cell specimens, yet its application to tissues has been hindered, primarily due to challenges posed by tissue thickness. Advances in sample vitrification, cryo-focused ion beam (cryo-FIB) milling, and lift-out techniques have substantially improved tissue preparation, enabling thin, electron microscopy-compatible samples. Furthermore, the integration of automation, complementary imaging modalities, and AI has streamlined imaging workflows and data analysis. This review highlights these technological developments, their implications for tissue analysis, and the future potential of cryo-ET in advancing structural biology and biomedical research.

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来源期刊

Current opinion in structural biology 生物-生化与分子生物学

CiteScore

12.20

自引率

2.90%

发文量

179

审稿时长

6-12 weeks

期刊介绍： Current Opinion in Structural Biology (COSB) aims to stimulate scientifically grounded, interdisciplinary, multi-scale debate and exchange of ideas. It contains polished, concise and timely reviews and opinions, with particular emphasis on those articles published in the past two years. In addition to describing recent trends, the authors are encouraged to give their subjective opinion of the topics discussed. In COSB, we help the reader by providing in a systematic manner: 1. The views of experts on current advances in their field in a clear and readable form. 2. Evaluations of the most interesting papers, annotated by experts, from the great wealth of original publications. [...] The subject of Structural Biology is divided into twelve themed sections, each of which is reviewed once a year. Each issue contains two sections, and the amount of space devoted to each section is related to its importance. -Folding and Binding- Nucleic acids and their protein complexes- Macromolecular Machines- Theory and Simulation- Sequences and Topology- New constructs and expression of proteins- Membranes- Engineering and Design- Carbohydrate-protein interactions and glycosylation- Biophysical and molecular biological methods- Multi-protein assemblies in signalling- Catalysis and Regulation