光交联剂提高结构信息从交联质谱

IF 6.1 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current opinion in structural biology Pub Date : 2025-07-04 DOI:10.1016/j.sbi.2025.103102

Anthony Ciancone, Francis J. O'Reilly

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引用次数: 0

摘要

交联质谱法已成为结构生物学研究的有力工具。该技术利用化学交联剂捕获蛋白质残基之间的空间接近度，以探测在接近天然条件下蛋白质组装的组织、化学计量和灵活性。光交联试剂在交联质谱中的应用越来越广泛，其化学性质为研究动态蛋白质结构提供了显著的优势。本文综述了光交联剂在交联质谱分析中的基本原理、应用及发展前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Photo-crosslinkers boost structural information from crosslinking mass spectrometry

Crosslinking mass spectrometry has emerged as a powerful tool in structural biology. This technology utilizes chemical crosslinkers to capture spatial proximities between protein residues to probe the organization, stoichiometry, and flexibility of protein assemblies under near-native conditions. Photo-crosslinking reagents have become increasingly used in crosslinking MS, with chemical properties that offer significant advantages when studying dynamic protein structures. This review explores the fundamentals, applications, and future potential of photo-crosslinkers in crosslinking mass spectrometry.

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来源期刊

Current opinion in structural biology 生物-生化与分子生物学

CiteScore

12.20

自引率

2.90%

发文量

179

审稿时长

6-12 weeks

期刊介绍： Current Opinion in Structural Biology (COSB) aims to stimulate scientifically grounded, interdisciplinary, multi-scale debate and exchange of ideas. It contains polished, concise and timely reviews and opinions, with particular emphasis on those articles published in the past two years. In addition to describing recent trends, the authors are encouraged to give their subjective opinion of the topics discussed. In COSB, we help the reader by providing in a systematic manner: 1. The views of experts on current advances in their field in a clear and readable form. 2. Evaluations of the most interesting papers, annotated by experts, from the great wealth of original publications. [...] The subject of Structural Biology is divided into twelve themed sections, each of which is reviewed once a year. Each issue contains two sections, and the amount of space devoted to each section is related to its importance. -Folding and Binding- Nucleic acids and their protein complexes- Macromolecular Machines- Theory and Simulation- Sequences and Topology- New constructs and expression of proteins- Membranes- Engineering and Design- Carbohydrate-protein interactions and glycosylation- Biophysical and molecular biological methods- Multi-protein assemblies in signalling- Catalysis and Regulation