Ella Foster , Oliver Wardhana , Ziyu Zeng , Xin Lu
{"title":"前列腺癌细胞三维培养方法的比较分析。","authors":"Ella Foster , Oliver Wardhana , Ziyu Zeng , Xin Lu","doi":"10.1016/j.yexcr.2025.114666","DOIUrl":null,"url":null,"abstract":"<div><div>Three-dimensional (3D) cell culture models are increasingly utilized in cancer research to better replicate in vivo tumor microenvironments. This study examines the effects of different 3D scaffolding materials, including Matrigel, GelTrex, and the plant-based GrowDex, on prostate cancer cell lines, with a particular emphasis on neuroendocrine prostate cancer (NEPC). Four cell lines (LNCaP, LASCPC-01, PC-3, and KUCaP13) were cultured in these scaffolds using the sandwich method to evaluate spheroid formation, cell viability, and gene expression. The results revealed that while all scaffolds supported cell viability, spheroid formation varied significantly: Matrigel promoted the most consistent spheroid formation, exhibiting the best results for LASCPC-01 among the tested scaffolds, though spheroid formation for LASCPC-01 was generally more limited than for LNCaP. Gene expression analysis indicated a reduction in androgen receptor (<em>AR</em>) expression in LNCaP cells across all scaffolds using the sandwich method. However, further experiments using a mini-dome method revealed that the expression of AR signaling genes and neuroendocrine marker genes varied depending on the scaffolds and culture methods. These findings highlight the scaffold-dependent variability in 3D culture outcomes and emphasize the need for standardized methodologies to ensure consistency and relevance in prostate cancer research.</div></div>","PeriodicalId":12227,"journal":{"name":"Experimental cell research","volume":"450 2","pages":"Article 114666"},"PeriodicalIF":3.5000,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparative analysis of 3D culture methodologies in prostate cancer cells\",\"authors\":\"Ella Foster , Oliver Wardhana , Ziyu Zeng , Xin Lu\",\"doi\":\"10.1016/j.yexcr.2025.114666\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Three-dimensional (3D) cell culture models are increasingly utilized in cancer research to better replicate in vivo tumor microenvironments. This study examines the effects of different 3D scaffolding materials, including Matrigel, GelTrex, and the plant-based GrowDex, on prostate cancer cell lines, with a particular emphasis on neuroendocrine prostate cancer (NEPC). Four cell lines (LNCaP, LASCPC-01, PC-3, and KUCaP13) were cultured in these scaffolds using the sandwich method to evaluate spheroid formation, cell viability, and gene expression. The results revealed that while all scaffolds supported cell viability, spheroid formation varied significantly: Matrigel promoted the most consistent spheroid formation, exhibiting the best results for LASCPC-01 among the tested scaffolds, though spheroid formation for LASCPC-01 was generally more limited than for LNCaP. Gene expression analysis indicated a reduction in androgen receptor (<em>AR</em>) expression in LNCaP cells across all scaffolds using the sandwich method. However, further experiments using a mini-dome method revealed that the expression of AR signaling genes and neuroendocrine marker genes varied depending on the scaffolds and culture methods. These findings highlight the scaffold-dependent variability in 3D culture outcomes and emphasize the need for standardized methodologies to ensure consistency and relevance in prostate cancer research.</div></div>\",\"PeriodicalId\":12227,\"journal\":{\"name\":\"Experimental cell research\",\"volume\":\"450 2\",\"pages\":\"Article 114666\"},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2025-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Experimental cell research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0014482725002666\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CELL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental cell research","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0014482725002666","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
Comparative analysis of 3D culture methodologies in prostate cancer cells
Three-dimensional (3D) cell culture models are increasingly utilized in cancer research to better replicate in vivo tumor microenvironments. This study examines the effects of different 3D scaffolding materials, including Matrigel, GelTrex, and the plant-based GrowDex, on prostate cancer cell lines, with a particular emphasis on neuroendocrine prostate cancer (NEPC). Four cell lines (LNCaP, LASCPC-01, PC-3, and KUCaP13) were cultured in these scaffolds using the sandwich method to evaluate spheroid formation, cell viability, and gene expression. The results revealed that while all scaffolds supported cell viability, spheroid formation varied significantly: Matrigel promoted the most consistent spheroid formation, exhibiting the best results for LASCPC-01 among the tested scaffolds, though spheroid formation for LASCPC-01 was generally more limited than for LNCaP. Gene expression analysis indicated a reduction in androgen receptor (AR) expression in LNCaP cells across all scaffolds using the sandwich method. However, further experiments using a mini-dome method revealed that the expression of AR signaling genes and neuroendocrine marker genes varied depending on the scaffolds and culture methods. These findings highlight the scaffold-dependent variability in 3D culture outcomes and emphasize the need for standardized methodologies to ensure consistency and relevance in prostate cancer research.
期刊介绍:
Our scope includes but is not limited to areas such as: Chromosome biology; Chromatin and epigenetics; DNA repair; Gene regulation; Nuclear import-export; RNA processing; Non-coding RNAs; Organelle biology; The cytoskeleton; Intracellular trafficking; Cell-cell and cell-matrix interactions; Cell motility and migration; Cell proliferation; Cellular differentiation; Signal transduction; Programmed cell death.