使用Trasis AllinOne模块全自动盒式生产[18F]白蛋白

IF 0.9 4区 医学 Q4 BIOCHEMICAL RESEARCH METHODS
Falguni Basuli, Jianfeng Shi, Xiang Zhang, Rolf E. Swenson
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引用次数: 0

摘要

多肽和蛋白质的氟-18标记通常通过间接标记方法进行。在这种标记方法中,首先制备标记的假体基团,然后将其偶联到感兴趣的蛋白质和肽上。6-[18F]氟烟酸-2,3,5,6-四氟苯基酯是一种有用的间接标记基。我们最近开发了一种有效的放射性标记方法,“Sep-Pak上的氟化”,可以在10分钟内制备出具有高放射化学产量和纯度的假体基团。各种生物分子已被放射性标记使用这个假基团。放射性标签程序要么是手动的,要么是半自动的。然而,全自动合成方法对于成功的临床翻译至关重要。因此,我们开发了一种全自动可重复的放射性标记方法,使用Trasis AllinOne模块制备氟-18标记的白蛋白。手术在50分钟内完成。总放射化学收率为25%-36%(衰变校正,n = 6),使用1毫克白蛋白,放射化学纯度为98%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Fully Automated Cassette-Based Production of [18F]Albumin Using the Trasis AllinOne Module

Fully Automated Cassette-Based Production of [18F]Albumin Using the Trasis AllinOne Module

Fluorine-18 labeling of peptides and proteins is typically performed by an indirect labeling method. In this labeling approach, a labeled prosthetic group is prepared first and then conjugated to the proteins and peptides of interest. 6-[18F]fluoronicotinic acid-2,3,5,6-tetrafluorophenyl ester is a useful prosthetic group for indirect labeling. We have recently developed an efficient radiolabeling method, “fluorination on Sep-Pak,” that enables the preparation of this prosthetic group with high radiochemical yield and purity in under 10 min. A variety of biomolecules have been radiolabeled using this prosthetic group. The radiolabeling procedure was either manual or semiautomated. However, a fully automated synthesis method is essential for successful clinical translation. Therefore, we developed a fully automated reproducible radiolabeling method to prepare fluorine-18–labeled albumin using the Trasis AllinOne module. The procedure was completed in 50 min. The overall radiochemical yield was 25%–36% (decay-corrected, n = 6) using 1 mg of albumin with a radiochemical purity > 98%.

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来源期刊
CiteScore
3.30
自引率
0.00%
发文量
57
审稿时长
1 months
期刊介绍: The Journal of Labelled Compounds and Radiopharmaceuticals publishes all aspects of research dealing with labeled compound preparation and applications of these compounds. This includes tracer methods used in medical, pharmacological, biological, biochemical and chemical research in vitro and in vivo. The Journal of Labelled Compounds and Radiopharmaceuticals devotes particular attention to biomedical research, diagnostic and therapeutic applications of radiopharmaceuticals, covering all stages of development from basic metabolic research and technological development to preclinical and clinical studies based on physically and chemically well characterized molecular structures, coordination compounds and nano-particles.
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