Thi Quy Nguyen, Thi Quynh Nhu Tran, Seon Hui Jang, Seon Ah Park, Seong Kyu Han
{"title":"香茅醇对幼年小鼠三叉神经尾侧亚核明胶质神经元的抑制作用。","authors":"Thi Quy Nguyen, Thi Quynh Nhu Tran, Seon Hui Jang, Seon Ah Park, Seong Kyu Han","doi":"10.4196/kjpp.25.039","DOIUrl":null,"url":null,"abstract":"<p><p>The substantia gelatinosa (SG) of the trigeminal subnucleus caudalis (Vc) serves as the primary relay point for orofacial nociceptive inputs transmitted <i>via</i> thin myelinated Aδ and unmyelinated C primary afferent fibers. Citronellol is a monoterpenoid alcohol found in the essential oil of various medicinal plants, such as <i>Cymbopogon citratus</i>. It has been shown to be able to alleviate orofacial pain. However, the precise mechanism by which citronellol modulates SG neurons in the Vc remains unclear. To investigate this, the whole-cell patch-clamp technique was used to examine antinociceptive effects of citronellol on SG neurons in the Vc of mice. In a high-chloride pipette solution, citronellol consistently induced inward currents which persisted even in the presence of tetrodotoxin (a voltage-gated Na<sup>+</sup> channel blocker), 6-cyano-7-nitroquinoxaline-2,3-dione (a non-<i>N</i>-methyl-d-aspartate glutamate receptor antagonist), and DL-2-amino-5-phosphonopentanoic acid (an <i>N</i>-methyl-d-aspartate receptor antagonist). Nevertheless, citronellol-induced inward currents were partially inhibited by picrotoxin, a GABA<sub>A</sub> receptor antagonist, or strychnine, a glycine receptor antagonist. Citronellol-induced inward currents were almost fully blocked when both strychnine and picrotoxin were applied together. In addition, citronellol enhanced both GABA-induced inward currents and glycine-induced inward currents. These findings suggest that citronellol can mediate inhibitory effects of GABA and glycine on SG neurons in the Vc and serve as a potential herbal treatment for orofacial pain.</p>","PeriodicalId":54746,"journal":{"name":"Korean Journal of Physiology & Pharmacology","volume":" ","pages":""},"PeriodicalIF":1.6000,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Inhibitory action of citronellol on the substantia gelatinosa neurons of the trigeminal subnucleus caudalis in juvenile mice.\",\"authors\":\"Thi Quy Nguyen, Thi Quynh Nhu Tran, Seon Hui Jang, Seon Ah Park, Seong Kyu Han\",\"doi\":\"10.4196/kjpp.25.039\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The substantia gelatinosa (SG) of the trigeminal subnucleus caudalis (Vc) serves as the primary relay point for orofacial nociceptive inputs transmitted <i>via</i> thin myelinated Aδ and unmyelinated C primary afferent fibers. Citronellol is a monoterpenoid alcohol found in the essential oil of various medicinal plants, such as <i>Cymbopogon citratus</i>. It has been shown to be able to alleviate orofacial pain. However, the precise mechanism by which citronellol modulates SG neurons in the Vc remains unclear. To investigate this, the whole-cell patch-clamp technique was used to examine antinociceptive effects of citronellol on SG neurons in the Vc of mice. In a high-chloride pipette solution, citronellol consistently induced inward currents which persisted even in the presence of tetrodotoxin (a voltage-gated Na<sup>+</sup> channel blocker), 6-cyano-7-nitroquinoxaline-2,3-dione (a non-<i>N</i>-methyl-d-aspartate glutamate receptor antagonist), and DL-2-amino-5-phosphonopentanoic acid (an <i>N</i>-methyl-d-aspartate receptor antagonist). Nevertheless, citronellol-induced inward currents were partially inhibited by picrotoxin, a GABA<sub>A</sub> receptor antagonist, or strychnine, a glycine receptor antagonist. Citronellol-induced inward currents were almost fully blocked when both strychnine and picrotoxin were applied together. In addition, citronellol enhanced both GABA-induced inward currents and glycine-induced inward currents. These findings suggest that citronellol can mediate inhibitory effects of GABA and glycine on SG neurons in the Vc and serve as a potential herbal treatment for orofacial pain.</p>\",\"PeriodicalId\":54746,\"journal\":{\"name\":\"Korean Journal of Physiology & Pharmacology\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2025-07-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Korean Journal of Physiology & Pharmacology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.4196/kjpp.25.039\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"PHARMACOLOGY & PHARMACY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Korean Journal of Physiology & Pharmacology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.4196/kjpp.25.039","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
Inhibitory action of citronellol on the substantia gelatinosa neurons of the trigeminal subnucleus caudalis in juvenile mice.
The substantia gelatinosa (SG) of the trigeminal subnucleus caudalis (Vc) serves as the primary relay point for orofacial nociceptive inputs transmitted via thin myelinated Aδ and unmyelinated C primary afferent fibers. Citronellol is a monoterpenoid alcohol found in the essential oil of various medicinal plants, such as Cymbopogon citratus. It has been shown to be able to alleviate orofacial pain. However, the precise mechanism by which citronellol modulates SG neurons in the Vc remains unclear. To investigate this, the whole-cell patch-clamp technique was used to examine antinociceptive effects of citronellol on SG neurons in the Vc of mice. In a high-chloride pipette solution, citronellol consistently induced inward currents which persisted even in the presence of tetrodotoxin (a voltage-gated Na+ channel blocker), 6-cyano-7-nitroquinoxaline-2,3-dione (a non-N-methyl-d-aspartate glutamate receptor antagonist), and DL-2-amino-5-phosphonopentanoic acid (an N-methyl-d-aspartate receptor antagonist). Nevertheless, citronellol-induced inward currents were partially inhibited by picrotoxin, a GABAA receptor antagonist, or strychnine, a glycine receptor antagonist. Citronellol-induced inward currents were almost fully blocked when both strychnine and picrotoxin were applied together. In addition, citronellol enhanced both GABA-induced inward currents and glycine-induced inward currents. These findings suggest that citronellol can mediate inhibitory effects of GABA and glycine on SG neurons in the Vc and serve as a potential herbal treatment for orofacial pain.
期刊介绍:
The Korean Journal of Physiology & Pharmacology (Korean J. Physiol. Pharmacol., KJPP) is the official journal of both the Korean Physiological Society (KPS) and the Korean Society of Pharmacology (KSP). The journal launched in 1997 and is published bi-monthly in English. KJPP publishes original, peer-reviewed, scientific research-based articles that report successful advances in physiology and pharmacology. KJPP welcomes the submission of all original research articles in the field of physiology and pharmacology, especially the new and innovative findings. The scope of researches includes the action mechanism, pharmacological effect, utilization, and interaction of chemicals with biological system as well as the development of new drug targets. Theoretical articles that use computational models for further understanding of the physiological or pharmacological processes are also welcomed. Investigative translational research articles on human disease with an emphasis on physiology or pharmacology are also invited. KJPP does not publish work on the actions of crude biological extracts of either unknown chemical composition (e.g. unpurified and unvalidated) or unknown concentration. Reviews are normally commissioned, but consideration will be given to unsolicited contributions. All papers accepted for publication in KJPP will appear simultaneously in the printed Journal and online.