香茅醇对幼年小鼠三叉神经尾侧亚核明胶质神经元的抑制作用。

IF 1.6 4区 医学 Q3 PHARMACOLOGY & PHARMACY
Thi Quy Nguyen, Thi Quynh Nhu Tran, Seon Hui Jang, Seon Ah Park, Seong Kyu Han
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引用次数: 0

摘要

三叉神经尾侧亚核(Vc)的明胶质(SG)作为口面痛觉输入的主要中继点,通过薄髓鞘Aδ和无髓鞘C初级传入纤维传递。香茅醇是一种单萜类醇,存在于各种药用植物的精油中,如香茅。它已被证明能够减轻口腔面部疼痛。然而,香茅醇调节Vc中SG神经元的确切机制尚不清楚。为此,采用全细胞膜片钳技术检测香茅罗对小鼠Vc中SG神经元的抗伤害感受作用。在高氯移液溶液中,香橼醇持续诱导向内电流,即使存在河蟹毒素(电压门控Na+通道阻滞剂),6-氰基-7-硝基喹啉-2,3-二酮(非n-甲基-d-天冬氨酸受体拮抗剂)和dl -2-氨基-5-磷酸戊酸(n-甲基-d-天冬氨酸受体拮抗剂)也持续存在。然而,香茅醇诱导的内向电流被GABAA受体拮抗剂微毒素或士的宁(甘氨酸受体拮抗剂)部分抑制。当士的宁和微毒素同时应用时,香茅醇诱导的内向电流几乎完全阻断。此外,香茅醇增强了gaba诱导的内向电流和甘氨酸诱导的内向电流。这些发现表明香茅醇可以介导GABA和甘氨酸对Vc中SG神经元的抑制作用,并可能作为一种治疗口面部疼痛的潜在草药。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Inhibitory action of citronellol on the substantia gelatinosa neurons of the trigeminal subnucleus caudalis in juvenile mice.

The substantia gelatinosa (SG) of the trigeminal subnucleus caudalis (Vc) serves as the primary relay point for orofacial nociceptive inputs transmitted via thin myelinated Aδ and unmyelinated C primary afferent fibers. Citronellol is a monoterpenoid alcohol found in the essential oil of various medicinal plants, such as Cymbopogon citratus. It has been shown to be able to alleviate orofacial pain. However, the precise mechanism by which citronellol modulates SG neurons in the Vc remains unclear. To investigate this, the whole-cell patch-clamp technique was used to examine antinociceptive effects of citronellol on SG neurons in the Vc of mice. In a high-chloride pipette solution, citronellol consistently induced inward currents which persisted even in the presence of tetrodotoxin (a voltage-gated Na+ channel blocker), 6-cyano-7-nitroquinoxaline-2,3-dione (a non-N-methyl-d-aspartate glutamate receptor antagonist), and DL-2-amino-5-phosphonopentanoic acid (an N-methyl-d-aspartate receptor antagonist). Nevertheless, citronellol-induced inward currents were partially inhibited by picrotoxin, a GABAA receptor antagonist, or strychnine, a glycine receptor antagonist. Citronellol-induced inward currents were almost fully blocked when both strychnine and picrotoxin were applied together. In addition, citronellol enhanced both GABA-induced inward currents and glycine-induced inward currents. These findings suggest that citronellol can mediate inhibitory effects of GABA and glycine on SG neurons in the Vc and serve as a potential herbal treatment for orofacial pain.

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来源期刊
Korean Journal of Physiology & Pharmacology
Korean Journal of Physiology & Pharmacology PHARMACOLOGY & PHARMACY-PHYSIOLOGY
CiteScore
3.20
自引率
5.00%
发文量
53
审稿时长
6-12 weeks
期刊介绍: The Korean Journal of Physiology & Pharmacology (Korean J. Physiol. Pharmacol., KJPP) is the official journal of both the Korean Physiological Society (KPS) and the Korean Society of Pharmacology (KSP). The journal launched in 1997 and is published bi-monthly in English. KJPP publishes original, peer-reviewed, scientific research-based articles that report successful advances in physiology and pharmacology. KJPP welcomes the submission of all original research articles in the field of physiology and pharmacology, especially the new and innovative findings. The scope of researches includes the action mechanism, pharmacological effect, utilization, and interaction of chemicals with biological system as well as the development of new drug targets. Theoretical articles that use computational models for further understanding of the physiological or pharmacological processes are also welcomed. Investigative translational research articles on human disease with an emphasis on physiology or pharmacology are also invited. KJPP does not publish work on the actions of crude biological extracts of either unknown chemical composition (e.g. unpurified and unvalidated) or unknown concentration. Reviews are normally commissioned, but consideration will be given to unsolicited contributions. All papers accepted for publication in KJPP will appear simultaneously in the printed Journal and online.
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