[从细胞培养中鉴定支原体分离物的实验]。

Veterinarno-meditsinski nauki Pub Date : 1987-01-01
K Petkova, M Dilovski, Ia Bŭchvarova
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引用次数: 0

摘要

鉴定了从三个连续细胞系BHK-21、SPZF和TT中分离的多种支原体生物。根据培养、生化和血清学调查,分离株确定为牛头分枝杆菌、精氨酸分枝杆菌和laidlai分枝杆菌。用于培养细胞系的牛血清被证明是支原体生物污染的基本来源。这使得有必要对每批血清在用于实验室和生产需要之前进行支原体污染的初步研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Experiments to identify Mycoplasma isolates from cell cultures].

Identified were various species of Mycoplasma organisms isolated from three continuous cell lines--BHK-21, SPZF, and TT. On the base of cultural, biochemical, and serological investigations the isolates were defined as M. bovirhinis, M. arginini, and A. laidlawii. The bovine sera, used to culture the cell lines were shown to be the basic source of contamination with Mycoplasma organisms. This made it necessary to carry out a preliminary study on each batch of serum for Mycoplasma contamination prior to its use for laboratory and productional needs.

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